LOCUS       sequence-24574-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cloning vector containing attB flanked TXNL4B useful for 
            recombination based conversion of conventional vectors to Gateway 
            compatible destination vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pBZ-attBtxnl4b
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(543..567)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(593..612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(594..612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gap             636..646
                     /estimated_length=11
ORIGIN
        1 cgcggtggcg gccgctctag agcatatgac gtcggcgcca tgggcggccg ctgaattcat
       61 taatacaagt ttgtacaaaa aagcaggctc gatgagcttc ctcttgccca aactgaccag
      121 caagaaagaa gtagaccagg cgatcaaaag cactgcggag aaggtgttgg ttctcaggtt
      181 tgggagggac gaggaccccg tctgtctgca gctagatgat attctttcta agacctcttc
      241 tgacttaagt aaaatggctg ctatatacct ggtagatgtg gaccaaactg cagtttatac
      301 acagtatttt gacatcagtt atattccatc tactgtcttt ttcttcaatg ggcagcacat
      361 gaaagtggat tatggatctc cagatcacac taagtttgtg ggaagcttca aaaccaaaca
      421 agacttcata gatttgattg aagtaatcta tcgaggagca atgaggggga agcttattgt
      481 ccaaagtcct attgatccca agaatattcc caaatatgac cttctctatc aagacattta
      541 ggacccagct ttcttgtaca aagtggtcct cgaggatccg gtacccaatt cgccctatag
      601 tgagtcgtat tacgcgcgct cactggccgt cgtttnnnnn nnnnnntgnn tgggnaaaac
      661 cctnnnnnnn acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      721 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atg
//
