LOCUS       sequence-245740-        7704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_CyRPA-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2482..2499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2551..2606
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2605..2624)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4842..4860)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4928..5032
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5033..5101,5102..5893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5251..5270)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6064..6652
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6553..6572
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6806..6823
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6940..6961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6976..6981,6982..6999,7000..7006)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7014..7030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7019..7041
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7038..7054
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7038..7054
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7325..7704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccgagcgga agaacaagaa catcatcctg
      841 atcaacgacg agacaagcag cctgaacaac gccgcccagt gcagccagga cgtgcacttc
      901 gccttcaaga aagagctgta caagtactgc gtcgagcacg acatcaagaa caccgagagc
      961 cacgtgtaca tccagaagaa agaaaacaac cggtggatca gccagagcga gctgctgggc
     1021 aagttctaca agatcaagcc cgtgtccatc ttcaacttcg tggacgacaa ccagatcatc
     1081 ctcatcatct gcgagtacat caacaacgac aagaccaaca ccaaggactg ctaccggtgg
     1141 tcctcccagg acggcaccac cttcaccaaa gaaaacgtgg tcatcgacaa cgatatcttc
     1201 aacaacaaga actacgccag ctacagcagc gcccctctga agatcagcaa caaggcctac
     1261 ctgctgatct gcggcaccca cagcaaccag ctgaagaaca acaaaaacga ggatcacctg
     1321 gccagctgca ccgccagcga cgacgacggc agaaattggc ggacccagat caagatcaac
     1381 tacgccggct tccaggccga cagccccttc tttgccctga cccctcacat cttcaatgac
     1441 gagctgggct tctacttcta caccggcgtg aacattgcca ccaagagcac aggcggccag
     1501 tatatcgcct gcagcgtgga caacggcgcc aacattgcct tcaactgcaa cgacgtgaac
     1561 ctgttcaaag agaacaagct gctgcagaat atcgccaagc tgagcgacca ctacctgacc
     1621 aactacatcg acaaggatta cctggacctg tgctacctgt actacacaaa agaaaatgcc
     1681 atcatgatcg gctccccaaa gcacatctac aacgatcaca agtgctacaa ctccgccatc
     1741 atcaaagagg acgagagcac cttctttttc acctactaca aaggccacgg cctgcagaac
     1801 atgcacagca tccactacac ccagtacgat ggcgcgccgt cgacctccat cacggcctat
     1861 aagagtgagg gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg
     1921 cagggagagt tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc
     1981 tccctaaaga accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg
     2041 tccgaaacgc tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct
     2101 ggcaacctga ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg
     2161 atgaaagtga ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca
     2221 cccaagatga gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag
     2281 aaagtgattc aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt
     2341 gaagaggtca agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc
     2401 ctgcaccaca tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct
     2461 cctctggccc ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa
     2521 ttaaggatcc cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag
     2581 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg
     2641 tcgagatccc tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc
     2701 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     2761 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     2821 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     2881 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     2941 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     3001 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     3061 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     3121 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     3181 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     3241 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     3301 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     3361 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     3421 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     3481 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     3541 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     3601 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     3661 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     3721 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     3781 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     3841 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     3901 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     3961 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     4021 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     4081 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     4141 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     4201 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     4261 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     4321 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     4381 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     4441 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     4501 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4561 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4621 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4681 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4741 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     4801 gataagctgt caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt
     4861 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4921 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4981 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5041 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5101 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5161 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5221 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     5281 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5341 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5401 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5461 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5521 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     5581 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5641 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5701 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5761 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5821 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5881 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5941 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6001 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6061 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6121 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6181 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6241 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6301 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6361 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6421 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6481 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6541 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6601 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6661 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6721 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6781 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6841 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6901 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6961 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     7021 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     7081 ggtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
     7141 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
     7201 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
     7261 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
     7321 tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7381 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7441 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7501 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7561 catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc
     7621 gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca gtacatctac
     7681 gtattagtca tcgctattac catg
//
