LOCUS       sequence-245742-        8889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_GAMA-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3667..3684
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3736..3791
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3790..3809)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6027..6045)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6113..6217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6218..6286,6287..7078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6436..6455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7249..7837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7738..7757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7991..8008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8125..8146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8161..8166,8167..8184,8185..8191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8199..8215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8204..8226
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8223..8239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8223..8239
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        8510..8889
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccctgatca gaaaccccaa cagcaatagc
      841 caggccctgg tgcccgagca gaacaacaac cagaacggca agcccaacgg cctgagcaac
      901 aacaatgaca atacctgcga gatccagaaa atggccgagg aaatgatgga aaagctgctg
      961 aacgagaagg acgtgttcac cagcatcatg gaacccctgc agagcaagct gaatgacgac
     1021 tacctgtgca cccagcccaa gtacaccaac atctgcatca acgagaaaga caagacccct
     1081 ctgaccttcc cctgcacctc ccctaagtac gagaacctga tccacgagtt cacctacaag
     1141 aagctgtgcc acagcaagat cgccttcagc aacaccctgc tgaagtcctt catcgaccag
     1201 aacaaagagg aaaacacctt caacaatatc gcccagaact acaataccct gctcaactgc
     1261 atcgacgacg acctgaaaga gatctacaag agcagcatcg acctgttcag cgatctgaag
     1321 gacagcgtgg tggaaatcac cgagaaactg tggtccaaga ccatcaccga cgtgctgaag
     1381 aagagagagc agctggtcac cggcatcttt tgcgagctga gaaacggcaa caaggccagc
     1441 ctggtcgaga acagcctgag ctacgagaat ctgggcatcc tgaagatcaa caacgtgcag
     1501 ctgctcaatg aggcctacag cgccttctcc gactactact attacttccc catcttcgcc
     1561 accaagctgc tcgagaaggg cggcctcgtg gaacggctgg tgtccatcca cgaaaacctg
     1621 gccaactacc ggacccggaa catcctcaag aagattaacg accagtccaa gaacgaggtg
     1681 ctgaacaacg aagaactgct gaatagcctc agcaactaca agcaccacaa tcagaagaac
     1741 aacggcgcct tcgcccagct gaagggcatc aagatctctg ccctggaagc cgccagcaat
     1801 ggcaacaacg aggctgcctc tggatccgct ggcggcagag ccaatgagaa catcaatggc
     1861 gccgacaaca ccgccagaag cgtgaacgcc aacgaaaacg ccgcccaggc ccagaatgag
     1921 cagcagaatc agccacagaa ccagcagcag aaccacgctc agaaccaggc acagaatcag
     1981 gcccagaacc aggtgcagaa ccaggcccag aatcaggctc agaatcagat gcagaatcac
     2041 gctcagaatc aggctcagaa ccaggctcag aatcaggcac agaaccaggc ccagaaccag
     2101 gcacagaacg aagctcagaa tgaagcccag aacgaagcac agaaccagcc ccagaatcag
     2161 atgcagaacc aggctcagaa ccaggcccag aaccaggtgc agaatcaggc tcagaacgaa
     2221 gcccagaatc agcctcagaa cgaggcacac caccggcagc agaatgaggc caccaatctg
     2281 gccgccagag tggaacagaa caatcacatc agcagctcca ccaaccgggt cctgaacaat
     2341 cccctgtaca gcaccagctc cctgaaggcc aagaatgtgg cccagctcgt gaaggatctg
     2401 ctgcagtcca tcaacatcgt gaagtttgag aacaatgagc ccaccggcca gatcgacgaa
     2461 gagggaatca agaaactgat tcagaacagc ttcctggacc tgagcgataa caccatgctc
     2521 gtgcggctgc tgatcaagcc ccaggccgtg atcctgttca tcatccagag ctttatcatg
     2581 atgaccccta gccccacccg ggacgccaag accttctgta gaaaggccct cgtgaatggc
     2641 cagctgatcg acagctccga cctggacgtg gccagcgagg aagaggacct gatcaactcc
     2701 tttgccagca agtacaacct catctacgaa aagattaaga tggaagaact ccgcgagaac
     2761 gaacagaaca agaaggccct gaaaaccagc aagacccacc tgtccgctct ggaagtgcgg
     2821 aacacccaga acattgccca gggcatctct accgagaaca aactgaacag cgcccggaac
     2881 gccccactga tcgccgtggt cacagatagc gccagcgaaa ccaccgacga catcattcag
     2941 aacaacgtgg acctcagcgc cctgagcccc gacgtcgagc agacctttca gacactgaat
     3001 gccaagggcg gatctggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag
     3061 tcagcggagt tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga
     3121 tggaaggcag agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa
     3181 aaggtgtctg tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca
     3241 ctcacccttc agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg
     3301 actctggaca gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag
     3361 cccgacagca acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg
     3421 atcttgaagc aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg
     3481 caggcccctg aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg
     3541 gactccaaga tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg
     3601 gacgcccaga agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg
     3661 ggcccccacc accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga
     3721 cctcgacctc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     3781 tgtgtctctc actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga
     3841 gatctctagc tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc
     3901 tgccccttct tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa
     3961 ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg
     4021 tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg
     4081 gagcagactt tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc
     4141 tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg
     4201 gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag
     4261 agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc
     4321 ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca
     4381 acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc
     4441 tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt
     4501 gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct
     4561 gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt
     4621 gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata
     4681 aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa
     4741 accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag
     4801 aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg
     4861 ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag
     4921 gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga
     4981 gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg
     5041 ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg
     5101 tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa
     5161 gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca
     5221 caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag
     5281 gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca
     5341 cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca
     5401 tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg
     5461 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     5521 tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg
     5581 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg
     5641 tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg
     5701 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     5761 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     5821 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     5881 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     5941 atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac
     6001 atgagaattt tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6061 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6121 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6181 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6241 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6301 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6361 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6421 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     6481 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6541 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6601 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6661 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6721 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt
     6781 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6841 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6901 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6961 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7021 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7081 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7141 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7201 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7261 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7321 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7381 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7441 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7501 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7561 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7621 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7681 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7741 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7801 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7861 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7921 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7981 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8041 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8101 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8161 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8221 cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc
     8281 atgtttgaca gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag
     8341 aactggtagg tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc
     8401 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
     8461 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg acattgatta
     8521 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     8581 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     8641 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     8701 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     8761 atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     8821 cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     8881 attaccatg
//
