LOCUS       sequence-245745-        7617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_P38-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2395..2412
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2464..2519
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2518..2537)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4755..4773)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4841..4945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4946..5014,5015..5806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5164..5183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5977..6565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6466..6485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6719..6736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6853..6874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6889..6894,6895..6912,6913..6919)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6927..6943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6932..6954
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6951..6967
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6951..6967
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7238..7617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccatgcaca tcgacctgag cgaccccaga
      841 aagagcatct tcctgctgaa cgtgcaggcc ggcgacaccg tggtgtacac atgcccctac
      901 agcttcaaca aagaaatgca ggatatgtac gccaaagaga agcagttctt cgagaagcgg
      961 cagttctgct tcgagaacat cgtgatcaac cggaacatct tcagcctgcg ggactacatc
     1021 agaggcgcct acaacatcaa cagcggctac gtgaacaacg tgtacacctc cgagttcacc
     1081 gtgcctcccg tggtgctgat gaaccggcac ttcgagtgct actgctacat ggaagaacag
     1141 aacgccatcg tgaagaaaac cctgaagatc cacatcagca agggcgtcgt gcggaagatc
     1201 cccggctgcg acttcaacga cgagtacaga gagagcaccg ccatcaccac cttcagcaac
     1261 atgaaccgga aggtggtcaa ggcctgcgac agctacccca agggcggcga tacaatcgcc
     1321 ctgctgtgcc ccatcaacta cgccgtgaag cccgacggct gcttcagcaa ggtgtacgtg
     1381 aagaaggaca acatcgagaa cgacaagaac aagctcgagg aacggttcaa cgtggcccgg
     1441 aagttcgaag aggacaagta caagatcgtg ggcgtggaaa ccctgttcag agagaagctg
     1501 gtcacctacg gcgacgagca ctacaagttc accaagatcc ctgagacaaa cgacgagatc
     1561 gccttcacct gtacctgtca ggccaacgac ctgtccgaca acctgatgat gaatgtgtac
     1621 atcaacgaga actacgacaa gttcgtgaag cacagcagca acgacaatgt cgagtacaac
     1681 aagaagaaga tcagcagcag ctacaacacc gtgtccgaag agattaagta cggcaacggc
     1741 gctggcgcgc cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc
     1801 tccttcccac tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag
     1861 aaggctcctt cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg
     1921 cagaagtcta ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag
     1981 ataccccagg tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga
     2041 gggatactgt atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac
     2101 actttgacct gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag
     2161 gagaatcagg aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa
     2221 gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc
     2281 caggttttat ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag
     2341 atgctgtgga accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac
     2401 catcaccacc attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg
     2461 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     2521 tcggaaggac atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta
     2581 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc
     2641 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
     2701 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc
     2761 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg
     2821 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac
     2881 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca
     2941 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca
     3001 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt
     3061 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata
     3121 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg
     3181 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag
     3241 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct
     3301 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt
     3361 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg
     3421 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca
     3481 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg
     3541 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca
     3601 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag
     3661 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac
     3721 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca
     3781 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc
     3841 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac
     3901 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa
     3961 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc
     4021 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg
     4081 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat
     4141 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta
     4201 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     4261 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     4321 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     4381 tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta
     4441 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct
     4501 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4561 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     4621 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4681 aatctgtatc cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc
     4741 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4801 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4861 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4921 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4981 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5041 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5101 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5161 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     5221 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5281 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5341 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5401 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5461 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     5521 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5581 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5641 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5701 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5761 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5821 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5881 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5941 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6001 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6061 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6121 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6181 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6241 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6301 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6361 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6421 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6481 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6541 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6601 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6661 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6721 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6781 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6841 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     6901 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6961 ctatgaccat gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc
     7021 ttatcatcgc agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta
     7081 tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat tggttatata
     7141 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
     7201 atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat
     7261 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     7321 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     7381 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     7441 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
     7501 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     7561 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatg
//
