LOCUS       sequence-24575-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET19b converted Gateway Destination vector for expressing His 
            tagged fusion proteins in bacteria. See note in comments section 
            below regarding the mutated enterokinase site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZY19
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             161..190
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    227..351
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gccngnggcg cggtgagccg gcccgatgcg tccggcgtag aggatcgaga tctcgatccc
       61 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata taccatgggc catcatcatc atcatcatca
      181 tcatcatcac agcagcggcc atatcgacgt cgacgacaag cataatacaa gtttgtacaa
      241 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
      301 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat gaatcaacta
      361 cttagatggt attagtgacc tgtagtcgac cgacagcctt ccaaatgttc ttcgggtgat
      421 gctgccaact tagtcgaccg acagccttcc aaatgttctt ctcaaacgga atcgtcgtat
      481 ccagcctact cgctattgtc ctcaatgccg tattaaatca taaaaagaaa taagaaaaag
      541 aggtgcgagc ctcttttttg tgtgacaaaa taaaaacatc tacctattca tatacgctag
      601 tgtcatagtc ctgaaaatca tcgtcancnn ngaacaattt cacacctgnt tatacttttc
      661 tcttacaagt cgttcggctt catctggatt ttcagcctct atacttacta aacgtgataa
      721 agtttctgta atttctactg tatcgacctg canacnggct gtgtataagg gagcctgaca
      781 ttatatcccc agacatcagg taatggcgtt ttgngtcntt
//
