LOCUS       sequence-245753-        7239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP4-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2397..2414
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2466..2521
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2520..2539)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4757..4775)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4843..4947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4948..5016,5017..5808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5166..5185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5979..6567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6468..6487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6721..6738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6855..6876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6891..6896,6897..6914,6915..6921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6929..6945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6934..6956
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6953..6969
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6953..6969
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccaacagcc
     1201 tgtacaactt caagaacggc aacaacgtgc tgacccacaa gcggaacatg ggcgacaaga
     1261 acaacggcaa gacctacaac aacgccggca agggcgaccc caagggcatc gacaacagca
     1321 acctgcctcc cgacagcagc aactctggca acaacaaggg cgtgaacgac gtgatcgccg
     1381 gcctgggcaa agatgtgcct ggcctggccg gaaatgatgc cagcgctagc tctacaggcg
     1441 gagccggaag cacagccaag cctatcgcca gccccgacaa caccaatctg ggcatggacg
     1501 aggaaaacac aaacgccggc gaggacgatt acagcgacag cgacgagtac gaggacgaag
     1561 aggacgacac ctacgagctg agcggcctgg acgtgaacaa tgtgaacctg tgcctggaca
     1621 acaatggcgg ctgcggcgac gacaagatct gcgagtacct gggcaaggga atcgtgaagt
     1681 gcctgtgcaa ggccggctac aagctgatcg gcaccgactg cgtgaaggcc agcgactcct
     1741 cttctggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     1801 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     1861 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     1921 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     1981 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     2041 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     2101 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     2161 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     2221 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     2281 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     2341 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     2401 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     2461 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     2521 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     2581 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     2641 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     2701 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     2761 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     2821 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     2881 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     2941 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     3001 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     3061 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     3121 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     3181 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     3241 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     3301 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     3361 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     3421 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     3481 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     3541 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     3601 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     3661 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     3721 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     3781 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     3841 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     3901 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     3961 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     4021 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     4081 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     4141 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     4201 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4261 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4321 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     4381 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     4441 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     4501 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     4561 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     4621 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     4681 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     4741 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4801 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4861 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4921 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4981 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5041 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5101 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5161 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     5221 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5281 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5341 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5401 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5461 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     5521 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5581 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5641 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5701 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5761 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5821 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5881 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5941 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6001 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6061 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6121 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6181 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6241 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6301 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6361 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6421 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6481 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6541 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6601 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6661 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6721 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6781 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6841 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6901 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6961 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     7021 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     7081 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     7141 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     7201 acatttatat tggctcatgt ccaatatgac cgccatgtt
//
