LOCUS       sequence-245759-        8058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP3.5-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1210..1219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2836..2853
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2905..2960
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2959..2978)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5196..5214)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5282..5386
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5387..5455,5456..6247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5605..5624)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6418..7006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6907..6926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7160..7177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7294..7315
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7330..7335,7336..7353,7354..7360)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7368..7384
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7373..7395
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7392..7408
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7392..7408
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7679..8058
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccaaggaca tcgaccacat cagcaacctg
      841 gccaacaaag agaagcccca gctgcggaac ggcctgagca gcaagaatat cgccacccag
      901 aacggcaagt ccaacctgga aatccggaac aactacgaca accacgccga caagaacaac
      961 gagatcagcg gcaacatcga gatggtggaa aagagcgccg agagcctgga acagctggaa
     1021 aagttccagg tggaagccca ggacgacgct aacaaagagg ataagaccaa agagatcgag
     1081 cgcgccaaga aggccaagaa ccaggccgag atcctgctga agaaagccga ggaatacgtg
     1141 aagagagccg gcaaggccgt cgagagggcc aagagcgtgg aaagcgtgaa cgagaaagcc
     1201 aagctgagcg ccgccatggt ggaagaggaa gagaacaacg ccaagaaagc cctggaagtg
     1261 gccaagaagc tggtcgagga aatcggcatc gagatcgagg ccaccagaac cgcccctagc
     1321 ctggacattg ccaagaaaaa ggccaacaac gccgaaacca gcctgaagct ggtggaacag
     1381 caggcccaga ttgccgaggt ggccgccgtg aatgccgaac tggccgtgtc tcacgtggaa
     1441 cgggcctgga ttgaggccaa gatcgccagc tacctggaaa agattgaggt gctgaatgag
     1501 agggctaaga aggcccagca gaaaaccaga gaggccaaag agcgggccga taaggccgtg
     1561 caggccgtga caagcgtgga agccatcaac gagaacgcta agaacgccgc cacccggacc
     1621 agagagaagg ccagagctgt cgaggcctcc ctgaagattg tggaagccga agtggctgtg
     1681 gccgagaagg cctccattga ggccgagaaa gctgccaccc tgaagggcga gatcggcaaa
     1741 gaactggaac agaccgagat caccaccgaa gagaagcggg ccaaagtgga agagacagtg
     1801 aagggcaaga caagagaggt cgaagaggcc accgccaagg tcgaggccgc cacagaagaa
     1861 gccgaaaagg ccgccaaaga ggctgaggcc gcctccaaag agacagagtc cgccagcaaa
     1921 gaagctgaga caccctccga ggaagccatt accaccagcg agcaggccag caccgccgag
     1981 tctcagaata gagcccaggt cggaaacaac gctgccaaag aacgggacga caccgacgtg
     2041 cacaaaggca aggacaaaga gcagatcgac aagggcgaaa tcaaagagaa aagcctgcag
     2101 cagaagaacg gcgaggaagt caagaacgac cagcagcagg acaagtccag catctacgag
     2161 ttcatcacca tgctgatcac ccctggcgcg ccgtcgacct ccatcacggc ctataagagt
     2221 gagggggagt cagcggagtt ctccttccca ctcaaccttg gagaggaaag cctgcaggga
     2281 gagttgagat ggaaggcaga gaaggctcct tcttcccagt cctggatcac cttctcccta
     2341 aagaaccaaa aggtgtctgt gcagaagtct actagcaacc ccaagttcca gctgtccgaa
     2401 acgctcccac tcacccttca gataccccag gtctcccttc agtttgctgg ttctggcaac
     2461 ctgaccctga ctctggacag agggatactg tatcaggaag tgaacctggt ggtgatgaaa
     2521 gtgactcagc ccgacagcaa cactttgacc tgtgaggtga tgggacccac ctcacccaag
     2581 atgagactga tcttgaagca ggagaatcag gaggccaggg tctccaggca ggagaaagtg
     2641 attcaagtgc aggcccctga agcaggggtg tggcaatgtc tactgagtga aggtgaagag
     2701 gtcaagatgg actccaagat ccaggtttta tccaaagggt tgaattccgg cagcctgcac
     2761 cacatcctgg acgcccagaa gatgctgtgg aaccacaggg acaggaacct gcctcctctg
     2821 gcccctctgg gcccccacca ccatcaccac cattgagtct agacacgtga ttaattaagg
     2881 atcccccgac ctcgacctct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     2941 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttggtcgaga
     3001 tccctcggag atctctagct agaggatcga tccccgcccc ggacgaacta aacctgacta
     3061 cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca
     3121 acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt
     3181 ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct
     3241 ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta
     3301 actcttggct gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga
     3361 agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg
     3421 accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt
     3481 agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata
     3541 tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac
     3601 agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac
     3661 gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg
     3721 aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg
     3781 ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc
     3841 cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa
     3901 ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt
     3961 taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg
     4021 aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat
     4081 gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg
     4141 ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa
     4201 attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt
     4261 ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact
     4321 gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc
     4381 acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg
     4441 ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag
     4501 cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta
     4561 atgttgccat gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     4621 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4681 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     4741 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     4801 gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct atcctaattt
     4861 atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     4921 ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4981 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     5041 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5101 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgataag
     5161 ctgtcaaaca tgagaatttt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     5221 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5281 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5341 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5401 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5461 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5521 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5581 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     5641 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5701 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5761 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5821 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5881 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     5941 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6001 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6061 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6121 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6181 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6241 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6301 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6361 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7081 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7141 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7201 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7261 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7321 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7381 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga
     7441 ccaattctca tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg
     7501 caggcgcaga actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc
     7561 atattagtca ttggttatat agcataaatc aatattggct attggccatt gcatacgttg
     7621 tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga
     7681 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     7741 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     7801 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     7861 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     7921 gtgtatcata tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     7981 cattatgccc agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta
     8041 gtcatcgcta ttaccatg
//
