LOCUS       sequence-24576-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pKW32 converted Gateway Destination vector for expressing hemoglobin
            tagged fusion proteins in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZYHb
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        69..146
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(166..185)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tccnaacgcg cgaggcagct ctagatcatg cgcacccgtg gccaggaccc aacgctgccc
       61 gaaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg
      121 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
      181 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
      241 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
      301 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
      361 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
      421 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
      481 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
      541 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
      601 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
      661 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
      721 tctcggcgcg ttagcgtctg gctggctggc ataatatctc actcgcaatc aaattcagcc
      781 gatacggaac gggangcgat ggagtgcatg tccg
//
