LOCUS       sequence-245761-        7806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_MSP7.4-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2964..2981
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3033..3088
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3087..3106)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5324..5342)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5410..5514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5515..5583,5584..6375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5733..5752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6546..7134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7035..7054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7288..7305
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7422..7443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7458..7463,7464..7481,7482..7488)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7496..7512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7501..7523
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7520..7536
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7520..7536
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccaaggata
     1201 agtacgagga cgagcagaag aactacaaca accagcatgt gctgctgaac atcatcaagg
     1261 acaagctgga aaagctgcag ggcggcatca tcaccaacaa ggtgtccgac accctgaaca
     1321 aggacttcaa gagcctgaag aaacggatgg aagaactcga gaagtacgag aagaagtaca
     1381 ccgagggcaa catcgagaac atcctgaaga acgaggccga ggaaagcctg ggcgacaaga
     1441 aacagatcct gggcgtggac gaggatgagt acaactggga cttcctgggc cagggcaaca
     1501 ccatgaccaa gggcgaggac aacgaggaag gcgccgacga caacgaagat gaagagggcg
     1561 aagaggacgg caccgccctg gaactgaagt ctaagggcga gcaggacacc tccatgctgg
     1621 ctcagggcgc cgagaagatc agcaccgaac tccagggtgg cgcccagcct accagcgagt
     1681 ctagcaacga ggtgcaggaa cagagcggcg gcaccgatgt gcggaccaca agagatcctg
     1741 ccgtggccga ggccaagacc cctctggaaa attcccctag cgcccagagc aacaccccta
     1801 gcacaggcga gaatgaggcc aaggccgcca gccaggacca ggccaaagaa agacagcccg
     1861 tggaaagcgc cctggtcaat gccgagcacg gccctaccca gggcagcaat atcgccaccc
     1921 ctaaggtgca gtacctggat aagctgttcg acgactacct gatcgccagc gacaaaaaga
     1981 aagagatcaa cgtccacaag taccaccgga agtacaacga tttcaagaag gcctacgagt
     2041 tcaccatgaa cgataacgag tacaagatcg tgaaagagct gtttgacttc tacttcaaga
     2101 aaggcgagga agagtccagc aacgccgcca acctgatcaa cctgtttaag aaggtgctgg
     2161 acgataacgt gttccggaaa gaattcgaca acatcatgca cggcatctac ggcttcgcca
     2221 agcggtacac ctacctgcgg ggcgacagga ccaacgacga gaacgtgtac aaagaactgt
     2281 tcaagaacat gctgagcctg agcaacatcg tgggcgcgcc gtcgacctcc atcacggcct
     2341 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     2401 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     2461 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     2521 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     2581 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     2641 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     2701 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     2761 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     2821 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggca
     2881 gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac aggaacctgc
     2941 ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag acacgtgatt
     3001 aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt tcattgcaat
     3061 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     3121 ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg acgaactaaa
     3181 cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg
     3241 ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca
     3301 aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact
     3361 gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt
     3421 tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg
     3481 gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg
     3541 ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct
     3601 aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca
     3661 atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc
     3721 taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag
     3781 gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc
     3841 gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag
     3901 aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag
     3961 gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac
     4021 caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg
     4081 aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat
     4141 ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt
     4201 attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg
     4261 taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac
     4321 ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac
     4381 tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt
     4441 tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg
     4501 gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg
     4561 tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg
     4621 cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg
     4681 gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat
     4741 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4801 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     4861 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4921 cctaatctgt atccgggtag catatgctat cctaatagag attagggtag tatatgctat
     4981 cctaatttat atctgggtag catatactac ccaaatatct ggatagcata tgctatccta
     5041 atctatatct gggtagcata tgctatccta atctatatct gggtagcata ggctatccta
     5101 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     5161 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     5221 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc
     5281 atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt gatacgccta
     5341 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     5761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     6001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     6061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6661 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7201 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7261 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7321 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7381 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7441 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7501 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     7561 gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
     7621 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
     7681 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
     7741 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
     7801 catgtt
//
