LOCUS       sequence-245767-        7497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_MSP5-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2661..2678
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2730..2785
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2784..2803)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5021..5039)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5107..5211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5212..5280,5281..6072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5430..5449)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6243..6831
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6732..6751
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6985..7002
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7119..7140
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7155..7160,7161..7178,7179..7185)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7193..7209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7198..7220
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7217..7233
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7217..7233
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 agaactactt cgacggcagc ctggtcaacc tgaagctggg caactgcaac tgcctgagca
     1261 agcggttcct gagcaacgag gaaaacgacg ccaaccccaa cgccgccgac agcatgggca
     1321 atcacgccaa cgatgccgac agcctgacca gcagcaacac caagcacgag gacggcggcg
     1381 atcacacaag cagcgaggcc gagaaggacg aggccagcaa gcaggacagc aaagtgaacg
     1441 gcgagaacaa gcacaccgtg aacgagcaga gccagaacga cctgaacagc gaggaaggcc
     1501 acatcaagac cctggacaac aaggactcca acgacaagaa gatggaaagc cggaagtccg
     1561 aggccgaaat ctctgagcac aagcacaagc ccagcgacga ctccgagagc gagaagaaag
     1621 agtcctccaa gggcaacgac cctcaggaca gctccgacga caagtccagc gaagagggcg
     1681 tgaacaagaa taccatggaa gaacagcacg agaagaagaa catcaacccc gccgacggcg
     1741 acggcggaga caacaccctg tacctggaca atatcggcga ccaggtggcc cactacagcg
     1801 ccctgaagaa caaccggatc gagaagggcg tgaccgacaa catggtgctg tccgatatcg
     1861 ccagcggcaa caacgccaag agctgcgccg tgaacaatgg cggctgtggc gacgaccagc
     1921 tgtgcatcag aatcgacaac atcggcatca agtgcatctg caaagaggga cacctgttcg
     1981 gcaacaagtg tatcctgacc aagagcctgg gcgcgccgtc gacctccatc acggcctata
     2041 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     2101 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     2161 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     2221 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     2281 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     2341 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     2401 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     2461 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     2521 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     2581 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     2641 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     2701 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     2761 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     2821 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     2881 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     2941 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     3001 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     3061 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     3121 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     3181 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     3241 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     3301 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     3361 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     3421 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     3481 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     3541 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     3601 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     3661 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     3721 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     3781 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     3841 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     3901 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     3961 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     4021 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     4081 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     4141 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     4201 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     4261 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     4321 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     4381 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     4441 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4501 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     4561 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4621 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     4681 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     4741 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     4801 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     4861 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4921 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     4981 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     5041 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5101 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5161 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5221 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5281 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5341 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5401 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5461 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5521 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5581 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5641 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5701 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     5761 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5821 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5881 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5941 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6001 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6061 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6121 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6181 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6241 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6301 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6361 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6421 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6481 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6541 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6601 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6661 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6721 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6781 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6841 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6901 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6961 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     7021 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7081 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     7141 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     7201 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     7261 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     7321 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     7381 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     7441 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
