LOCUS       sequence-245769-        7899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP10-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2677..2694
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2746..2801
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2800..2819)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5037..5055)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5123..5227
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5228..5296,5297..6088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5446..5465)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6259..6847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6748..6767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7001..7018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7135..7156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7171..7176,7177..7194,7195..7201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7209..7225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7214..7236
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7233..7249
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7233..7249
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7520..7899
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gcctacatca acgacgtgaa ggacaccaac
      841 gacgtcaaga tctacaagca ggacaacgcc aagaacagcg ggaacaacaa caacgaggcc
      901 gtgaccgacg acggcgagga atgggacaac gacgacaacc acgccgacga gaacgacgag
      961 acagagaaca tcgacgacat ggataacaac aagaacaacc tgaacaccgc caacgtgatc
     1021 aagatgatgc agaagtacta cgtccggaag cggcccgaca acgcccacaa gaacgacatg
     1081 tacaccgtgg aacccttcca caccgcctac ctccagaagt acaagaacct gaaggaccac
     1141 agcaatatcg ccaacgacaa caataatgcc gatgagaacg tggaaaacga tgccaatcac
     1201 gccttcgact atatcgtcga gaacatgatc ccatttctgc tgagcaaaga ggacaacaat
     1261 caggccgcca ccggcgagga cgacaacaga gaaatggatg gcggcaaggg caccaagagc
     1321 aacgagggcc ctggcaacaa ccggatcgac aaggacccct ccaaagagca gagcggcgag
     1381 aagaacgagc ggagcgacga cagcagcagc aactctgccc acagcgaggg cgacagcccc
     1441 aatggcaaga gcaccagcaa cgagaagcgg cctcccaacg aggaaagcct gcacgagcag
     1501 acccctagag aggaaaccag ccacgccgag acaaccccta ccagcagcaa gagcagcgaa
     1561 gaggacaccc agaagaaaga aagccagacc ccaatggtgg accccagcga gcagatcaag
     1621 aaaaccctgc tgaaagaggg caaggacatc aaagagacaa ccagcctgat cgacaatgcc
     1681 gtgtacaatg tggaacaggt catcctcaag acccggttct acagcaccgc catccggaac
     1741 ttcgtgaact tcaaagtgaa ccacatctgc gagtacagca agtgcggcct gaacgcccgg
     1801 tgctacatca tcgagaagga caaagaggaa tgccgctgcc gggccaacta cgagcaggat
     1861 accagcgtgg aatacttcaa gtgcaagccc atgaccaagt gggactgcac catcaacaac
     1921 ggcaactgcg acaagaacgc cgagtgcacc atcgagaacg agaggatcaa gtgccagtgc
     1981 ggcttcaact acttcggcga cggcatcttc tgcgtgatgg gctctggcgc gccgtcgacc
     2041 tccatcacgg cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt
     2101 ggagaggaaa gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag
     2161 tcctggatca ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac
     2221 cccaagttcc agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt
     2281 cagtttgctg gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa
     2341 gtgaacctgg tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg
     2401 atgggaccca cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg
     2461 gtctccaggc aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt
     2521 ctactgagtg aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg
     2581 ttgaattccg gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg
     2641 gacaggaacc tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc
     2701 tagacacgtg attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta
     2761 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     2821 gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc
     2881 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     2941 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     3001 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     3061 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     3121 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     3181 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     3241 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     3301 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     3361 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     3421 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     3481 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     3541 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     3601 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     3661 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     3721 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     3781 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     3841 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     3901 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     3961 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     4021 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     4081 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     4141 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     4201 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     4261 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     4321 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     4381 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     4441 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     4501 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     4561 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     4621 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     4681 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     4741 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     4801 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     4861 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     4921 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     4981 atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct
     5041 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5101 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5161 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5221 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5281 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5341 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5401 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5461 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5521 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5581 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5641 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5701 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5761 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5821 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5881 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5941 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6001 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6061 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6121 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6181 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6241 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6301 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6361 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6421 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6481 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6541 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6601 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6661 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6721 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6781 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6841 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6901 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6961 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7021 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     7081 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7141 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7201 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     7261 caagctctag ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     7321 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     7381 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     7441 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     7501 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     7561 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     7621 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7681 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     7741 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     7801 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     7861 tggcagtaca tctacgtatt agtcatcgct attaccatg
//
