LOCUS       sequence-245771-        7707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_CyRPA-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2485..2502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2554..2609
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2608..2627)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4845..4863)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4931..5035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5036..5104,5105..5896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5254..5273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6067..6655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6556..6575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6809..6826
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6943..6964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6979..6984,6985..7002,7003..7009)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7017..7033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7022..7044
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7041..7057
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7041..7057
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7328..7707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccagctcta caaagaatgg cgccgtgatc
      841 ctgatcaacg acgacatcag caccgtgacc gacgagaagt actgtatgca ggacagctac
      901 ttcctgttcc acgacgagct gtacaagatc tgcatccagc acctcatcaa gaacgacatc
      961 gagatcagca tctacgtgca gaaagaagtg aagaacagct gggagacaca ggaagaactg
     1021 atcagcgacg tgaacaccaa gaagcccttc agcctgcaca cctacatcga cgacaatgag
     1081 atcgtgatca tcatgtgcaa atgcgtgaac gacctgagca tcgaccggaa cgagtgctac
     1141 agatgggaca gcaccaacgg caatttcttc aagaaagagg acatcaccat ccccgacagc
     1201 gccttcaaca agcggcggat gaccagctac accctgtacc ctctgaccat ctccaccaag
     1261 aaaatcctgc tgctgtgcgg catcaacatc gacccctacg tgccccagaa gaaagaagag
     1321 ttcatctact gcatcggcag cgaggaccgg ggcaagacct ggatgcggaa ggtgcagatc
     1381 atctacgagg gcttccagcc caatggcatc tactaccacc tgacccctca catcttcaac
     1441 gatgagctgg gcttccactt ctacagcctc gtgggcgcca acaatgctgg cgtgggcggc
     1501 gagtacatcg tgtgcaagcc tgagaagggc atgctgtacc ggatgaagtg cgagaacgcc
     1561 aacctgggca aagagggcaa gagcctgcag gacctgacca agatgaacgg ctactatatc
     1621 gcctcctacg tgaacaaaga aaagtccaat gagtgctacc tgtactacac cagcgagaat
     1681 gccatcatcc tgaaccccaa gaaacagggc tacctgatga atggctgcta cagcggcttc
     1741 ttcgtgaaga tcaacgaggc cagcaccctg tttatctacg cccctgacta cggcacctac
     1801 aacatccaca ccatcaagta catgcgctac gatggcgcgc cgtcgacctc catcacggcc
     1861 tataagagtg agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc
     1921 ctgcagggag agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc
     1981 ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag
     2041 ctgtccgaaa cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt
     2101 tctggcaacc tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg
     2161 gtgatgaaag tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc
     2221 tcacccaaga tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag
     2281 gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa
     2341 ggtgaagagg tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc
     2401 agcctgcacc acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg
     2461 cctcctctgg cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat
     2521 taattaagga tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa
     2581 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     2641 tggtcgagat ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa
     2701 acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg
     2761 gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac
     2821 aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac
     2881 tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct
     2941 ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg
     3001 ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc
     3061 gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc
     3121 taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta accctaattc
     3181 aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc
     3241 ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca
     3301 ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag
     3361 cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata
     3421 gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca
     3481 ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca
     3541 ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct
     3601 gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca
     3661 tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt
     3721 tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt
     3781 gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca
     3841 cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca
     3901 ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg
     3961 ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc
     4021 ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag
     4081 gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt
     4141 gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac
     4201 ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta
     4261 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     4321 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     4381 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     4441 tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta
     4501 tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     4561 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     4621 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4681 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     4741 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     4801 catgataagc tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct
     4861 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     4921 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4981 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5041 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5101 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5161 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5221 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     5281 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5341 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5401 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5461 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5521 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     5581 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5641 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5701 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5761 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5821 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5881 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     5941 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6001 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6061 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6121 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6181 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6241 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6301 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6361 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6421 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6481 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6541 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6601 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6661 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6721 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     6781 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     6841 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     6901 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     6961 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7021 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct
     7081 agaggtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg
     7141 ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg
     7201 caattagcca tattagtcat tggttatata gcataaatca atattggcta ttggccattg
     7261 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
     7321 ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     7381 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     7441 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     7501 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     7561 gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg
     7621 cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc
     7681 tacgtattag tcatcgctat taccatg
//
