LOCUS       sequence-24578-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pcDNA3 converted Gateway Destination vector for mammalian 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZY3
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    149..273
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cgggggcntt agcgagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa
       61 ttaatacgac tcactatagg gagacccaag cttggtaccg agctcggatc cactagtaac
      121 ggccgccagt gtgctggaat tgattaatac aagtttgtac aaaaaagctg aacgagaaac
      181 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      241 atactgtaaa acacaacata tccagtcact atgaatcaac tacttagatg gtattagtga
      301 cctgtagtcg accgacagcc ttccaaatgt tcttcgggtg atgctgccaa cttagtcgac
      361 cgacagcctt ccaaatgttc ttctcaaacg gaatcgtcgt atccagccta ctcgctattg
      421 tcctcaatgc cgtattaaat cataaaaaga aataagaaaa agaggtgcga gcctcttttt
      481 tgtgtgacaa aataaaaaca tctacctatt catatacgct agtgtcatag tcctgaaaat
      541 catctgcatc aagaacaatt tcacaactct tatacttttc tcttacaagt cgttcggctt
      601 catctggatt ttcagcctct atacttacta aacgtgataa agtttctgta atttctactg
      661 tatcgacctg cagactggct gtgtataagg gagcctgant tttcttcncc ccagaacatc
      721 aggttgatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttcccga
      781 taacggagac ggcac
//
