LOCUS       sequence-245790-       10955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FGFR2 N549K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-FGFR2-N549K
FEATURES             Location/Qualifiers
     source          1..10955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3844..3855
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(5915..5939)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5947..5965
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5990..6009)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5991..6009)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6015..6035)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             6089..6130
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6151..6718
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6168..6720
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6312..6329)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6539..6558
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6732..7331
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6732..6751)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7228..7248
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7345..7933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7398..7418)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7816..7827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8008..8241
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8360..8378)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8446..8550
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8551..8619,8620..9411)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8769..8788)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9582..10170
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10071..10090
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10324..10341
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10581..10602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10617..10622,10623..10640,10641..10647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10655..10671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10660..10682
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10679..10695
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10679..10695
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10745..10764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gtcagctggg gtcgtttcat ctgcctggtc
     3481 gtggtcacca tggcaacctt gtccctggcc cggccctcct tcagtttagt tgaggatacc
     3541 acattagagc cagaagagcc accaaccaaa taccaaatct ctcaaccaga agtgtacgtg
     3601 gctgcgccag gggagtcgct agaggtgcgc tgcctgttga aagatgccgc cgtgatcagt
     3661 tggactaagg atggggtgca cttggggccc aacaatagga cagtgcttat tggggagtac
     3721 ttgcagataa agggcgccac gcctagagac tccggcctct atgcttgtac tgccagtagg
     3781 actgtagaca gtgaaacttg gtacttcatg gtgaatgtca cagatgccat ctcatccgga
     3841 gatgatgagg atgacaccga tggtgcggaa gattttgtca gtgagaacag taacaacaag
     3901 agagcaccat actggaccaa cacagaaaag atggaaaagc ggctccatgc tgtgcctgcg
     3961 gccaacactg tcaagtttcg ctgcccagcc ggggggaacc caatgccaac catgcggtgg
     4021 ctgaaaaacg ggaaggagtt taagcaggag catcgcattg gaggctacaa ggtacgaaac
     4081 cagcactgga gcctcattat ggaaagtgtg gtcccatctg acaagggaaa ttatacctgt
     4141 gtggtggaga atgaatacgg gtccatcaat cacacgtacc acctggatgt tgtggagcga
     4201 tcgcctcacc ggcccatcct ccaagccgga ctgccggcaa atgcctccac agtggtcgga
     4261 ggagacgtag agtttgtctg caaggtttac agtgatgccc agccccacat ccagtggatc
     4321 aagcacgtgg aaaagaacgg cagtaaatac gggcccgacg ggctgcccta cctcaaggtt
     4381 ctcaaggccg ccggtgttaa caccacggac aaagagattg aggttctcta tattcggaat
     4441 gtaacttttg aggacgctgg ggaatatacg tgcttggcgg gtaattctat tgggatatcc
     4501 tttcactctg catggttgac agttctgcca gcgcctggaa gagaaaagga gattacagct
     4561 tccccagact acctggagat agccatttac tgcatagggg tcttcttaat cgcctgtatg
     4621 gtggtaacag tcatcctgtg ccgaatgaag aacacgacca agaagccaga cttcagcagc
     4681 cagccggctg tgcacaagct gaccaaacgt atccccctgc ggagacaggt aacagtttcg
     4741 gctgagtcca gctcctccat gaactccaac accccgctgg tgaggataac aacacgcctc
     4801 tcttcaacgg cagacacccc catgctggca ggggtctccg agtatgaact tccagaggac
     4861 ccaaaatggg agtttccaag agataagctg acactgggca agcccctggg agaaggttgc
     4921 tttgggcaag tggtcatggc ggaagcagtg ggaattgaca aagacaagcc caaggaggcg
     4981 gtcaccgtgg ccgtgaagat gttgaaagat gatgccacag agaaagacct ttctgatctg
     5041 gtgtcagaga tggagatgat gaagatgatt gggaaacaca agaatatcat aaaacttctt
     5101 ggagcctgca cacaggatgg gcctctctat gtcatagttg agtatgcctc taaaggcaac
     5161 ctccgagaat acctccgagc ccggaggcca cccgggatgg agtactccta tgacattaac
     5221 cgtgttcctg aggagcagat gaccttcaag gacttggtgt catgcaccta ccagctggcc
     5281 agaggcatgg agtacttggc ttcccaaaaa tgtattcatc gagatttagc agccagaaat
     5341 gttttggtaa cagaaaacaa tgtgatgaaa atagcagact ttggactcgc cagagatatc
     5401 aacaatatag actattacaa aaagaccacc aatgggcggc ttccagtcaa gtggatggct
     5461 ccagaagccc tgtttgatag agtatacact catcagagtg atgtctggtc cttcggggtg
     5521 ttaatgtggg agatcttcac tttagggggc tcgccctacc cagggattcc cgtggaggaa
     5581 ctttttaagc tgctgaagga aggacacaga atggataagc cagccaactg caccaacgaa
     5641 ctgtacatga tgatgaggga ctgttggcat gcagtgccct cccagagacc aacgttcaag
     5701 cagttggtag aagacttgga tcgaattctc actctcacaa ccaatgagga atacttggac
     5761 ctcagccaac ctctcgaaca gtattcacct agttaccctg acacaagaag ttcttgttct
     5821 tcaggagatg attctgtttt ttctccagac cccatgcctt acgaaccatg ccttcctcag
     5881 tatccacaca taaacggcag tgttaaaaca tagaacccag ctttcttgta caaagtggtg
     5941 atatccaatt aaccctcact aaaggtttaa gtgagttata gaagtagtac cctatagtga
     6001 gtcgtattac cacccaactt ttctatacaa agtggttgat atccagcaca gtggcggccg
     6061 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     6121 cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg
     6181 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     6241 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     6301 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     6361 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     6421 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     6481 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     6541 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     6601 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     6661 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     6721 tggccacaca tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6781 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     6841 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     6901 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     6961 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     7021 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7081 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7141 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7201 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7261 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7321 ccggtgccta aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     7381 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7441 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7501 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7561 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7621 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7681 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7741 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7801 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7861 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7921 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
     7981 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8041 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     8101 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8161 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8221 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     8281 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
     8341 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     8401 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8461 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8521 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8581 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8641 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8701 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8761 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     8821 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8881 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8941 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9001 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9061 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9121 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9181 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9241 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9301 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9361 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9421 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9481 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9601 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9661 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9721 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9781 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9841 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9901 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9961 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10021 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10081 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10141 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10201 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10261 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10321 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    10381 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
    10441 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10501 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
    10561 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    10621 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    10681 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
    10741 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
    10801 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
    10861 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    10921 tgtatttgtt aaatatgtac tacaaactta gtagt
//
