LOCUS       sequence-245791-       10153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter construct for the study of cis-repressive 
            activity in long noncoding RNAs. Also confers puromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Kirk JM, Kim SO, Inoue K, Smola MJ, Lee DM, Schertzer MD, Wooten JS,
            Baker AR, Sprague D, Collins DW, Horning CR, Wang S, Chen Q, Weeks 
            KM, Mucha PJ, Calabrese JM
  TITLE     Functional classification of long non-coding RNAs by k-mer content.
  JOURNAL   Nat Genet. 2018 Oct;50(10):1474-1482. doi: 
            10.1038/s41588-018-0207-8. Epub 2018 Sep 17.
  PUBMED    30224646
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0207-8"; journalName: "Nat Genet"; date: 
            "2018-10"; volume: "50"; issue: "10"; pages: "1474-1482"
COMMENT     Sequence Label: pTETRIS-cargo
FEATURES             Location/Qualifiers
     source          1..10153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      551..560
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             557..2209
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMGVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALYIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKEGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQCY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2253..2374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2615..2696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2937..3251)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(2940..2964)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3011..3029
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3047..3065
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3082..3100
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3118..3136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3154..3172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3189..3207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3225..3243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3268..3286)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     repeat_region   3691..3753
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3883..3900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3883..3899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3892..3914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4041..4496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4128..4147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4338..4359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4522..4626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4627..4695,4696..5487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4845..4864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5658..6246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6147..6166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6534..6555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6570..6575,6576..6593,6594..6600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6608..6624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6613..6635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6632..6648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6632..6648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   7139..7173
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    7936..8057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8104..8703)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8187..8207)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8684..8703
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(8710..8763)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(8800..9408)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     primer_bind     complement(9470..9489)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             complement(9520..9546)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(9526..9552)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      9555..9564
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             9592..9860
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(9873..10084)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg acctgcagcc tgtttcgaga attatcaaga tctggcctcg
      541 ggttggtaaa gccaccatgg aagatgccaa aaacattaag aagggcccag cgccattcta
      601 cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc gctacgccct
      661 ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta cctacgccga
      721 gtacttcgag atgggcgttc ggctggcaga agctatgaag cgctatgggc tgaatacaaa
      781 ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc
      841 cctgtacatc ggtgtggctg tggccccagc taacgacatc tacaacgagc gcgagctgct
      901 gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaaggaag ggctgcaaaa
      961 gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca tggatagcaa
     1021 gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt tgccacccgg
     1081 cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca tcgccctgat
     1141 catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc accgcaccgc
     1201 ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca tccccgacac
     1261 cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca cgctgggcta
     1321 cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg
     1381 cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat ttagcttctt
     1441 cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga tcgccagcgg
     1501 cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc acctaccagg
     1561 catccgccag tgctacggcc tgacagaaac aaccagcgcc attctgatca cccccgaagg
     1621 ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga
     1681 cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc
     1741 catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca tcgacaagga
     1801 cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact tcttcatcgt
     1861 ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag ccgaactgga
     1921 gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc tgcccgacga
     1981 cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga
     2041 gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc tgcgcggtgg
     2101 tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg cccgcaagat
     2161 ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtaat aattctagag
     2221 tcggggcggc cggccgcttc gagcagacat gataagatac attggtgagt ttggacaaac
     2281 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2341 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2401 gtttcaggtt cagggggagg tgtgggaggt tttttccggg ccatagagcc caccgcatcc
     2461 ccagcatgcc tgctattgtc ttcccatcac atgttctttc ctgcgttatc ccctgattct
     2521 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2581 gagcgcagcg agtcagtgag cgaggaagct cgaggcagtg aaaaaaatgc tttatttgtg
     2641 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2701 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2761 gcaagtaaga cctctacaat gtggtatggc tgattatgat cctctagaga tatcgtcgac
     2821 aagcttatcg ataaggcaca gtcgaattct cgagctagcg atatcgcggc cgcggatccg
     2881 atttaaattc gaattcgcta gctctagacc aaacggtacc gagctcgaat tctccaggcg
     2941 atctgacggt tcactaaacg agctctgctt atataggcct cccaccgtac acgcctacct
     3001 cgacatacgt tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat
     3061 agggataaac tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct
     3121 ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg gagtaaactc
     3181 gacatcgttc tctatcactg atagggagta aactcgacat acgttctcta tcactgatag
     3241 ggagtaaact cgagtgaaga cgaaagggcc tcgtgatacg cctatttccc cgtatccccc
     3301 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     3361 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
     3421 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
     3481 acatccctgg gggctttggg ggggggctgt ccctcataaa agttttgtta ctttatagaa
     3541 gaaattttga gtttttgttt tttttaataa ataaataaac ataaataaat tgtttgttga
     3601 atttattatt agtatgtaag tgtaaatata ataaaactta atatctattc aaattaataa
     3661 ataaacctcg atatacagac cgataaaaca catgcgtcaa ttttacacat gattatcttt
     3721 aacgtacgtc acaatatgat tatctttcta gggttaatct agctgcgtgt tctgcagcgt
     3781 gtcgagcatc ttcatctgct ccatcacgct gtaaaacaca tttgcaccgc gagtctgccc
     3841 gtcctccacg ggttcaaaaa cgtgaatgaa cgaggcgcgc tcactggccg tcgttttaca
     3901 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3961 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4021 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4081 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4141 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4201 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4261 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4321 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4381 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4441 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     4501 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4561 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4621 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4681 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4741 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4801 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4861 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4921 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4981 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5041 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5101 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5161 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5221 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5281 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5341 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5401 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5461 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5521 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5581 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6061 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6361 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6421 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6481 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     6541 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     6601 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     6661 aagcgcgccc gccgggtaac tcacggggta tccatgtcca tttctgcggc atccagccag
     6721 gatacccgtc ctcgctgacg taatatccca gcgccgcacc gctgtcatta atctgcacac
     6781 cggcacggca gttccggctg tcgccggtat tgttcgggtt gctgatgcgc ttcgggctga
     6841 ccatccggaa ctgtgtccgg aaaagccgcg acgaactggt atcccaggtg gcctgaacga
     6901 acagttcacc gttaaaggcg tgcatggcca caccttcccg aatcatcatg gtaaacgtgc
     6961 gttttcgctc aacgtcaatg cagcagcagt catcctcggc aaactctttc catgccgctt
     7021 caacctcgcg ggaaaaggca cgggcttctt cctccccgat gcccagatag cgccagcttg
     7081 ggcgatgact gagccggaaa aaagacccga cgatatgatc ctgatgcagc tagattaacc
     7141 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
     7201 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
     7261 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
     7321 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
     7381 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
     7441 agatatcagg gacagccccc ccccaaagcc cccagggatg taattacgtc cctcccccgc
     7501 tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc
     7561 cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc
     7621 tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag
     7681 ccataccaat gggccctaaa aaaggaatcc agtcaattcc ggggctaaac ctggctgcca
     7741 ctgtttcttt agggacttcg ttcctgtgag gacaggaaac cctgcaacct ggttgaccag
     7801 agcaagggat ataagaaccc agtttgggaa cactgctgtt taaatattaa acagggatgt
     7861 cttgtccccc ggaagggaga ccagatgagc ttctgataga ctaattgaca tcatttgagt
     7921 caattccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     7981 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     8041 gctgcaataa acaagtttag ttagttaacc acaaattttg taatccagag gttgattgtc
     8101 gactcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
     8161 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
     8221 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
     8281 gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc
     8341 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
     8401 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
     8461 gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc
     8521 gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg
     8581 gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt
     8641 gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt
     8701 catgaattca gggccgggat tctcctccac gtcaccgcat gttagaagac ttcctctgcc
     8761 ctctcgaaca gagagagacc ggccagaggg tttggccgcg cgcttgaatt tcgcgtaccg
     8821 ctctcgcgca atttcgagtg tcgagttcct gacacgctca aagcgttctt tgtccttctg
     8881 ccataggcta cgaacagcaa gcccacgcac cgaattgaat atcaaccaaa ggatatcttc
     8941 tgcatcatca cgcgaaagac cacggctcac cagaacacca agccacatat cctcgacgac
     9001 aaaccgattc ctctctaccg tgcgctgaat accctcgcgt aacgctggat cccggtcggc
     9061 agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg aggaaaaatt cggcggcgtc
     9121 gtccagcatt tgctggatga cgtcatcctc tggcttcaat ttcgctaatc gagcccgact
     9181 gcgttcggtg atctgttcgt aaagccattc aaaagtggca agcagaagct caagctttgt
     9241 cgggaaatga tggctctgcg ctcctctcga gacacctgca gcaccgggca catctgcgat
     9301 ccggaatccc gcgtaacctt tttcccgtaa aacccccagg gccgctgcaa tcaacttgcc
     9361 ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt ggactcatga tcacgggtct
     9421 gggtctcttt ggcatggtag atcttccaaa cttggacctg ggagtggaca cctgtggaga
     9481 gaaaggcaaa gtggatgtca tcggtctctg ctgcctcacc aaactagcgt aatctggaac
     9541 atcgtatggg taagccatgg cggctctaga gtaggcgccg gtcacagctt ggatctgtaa
     9601 cggcgcagaa cagaaaacga aacaaagacg tagagttgag caagcagggt caggcaaagc
     9661 gtggagagcc ggctgagtct aggtaggctc caagggagcg ccggacaaag gcccggtctc
     9721 gacctgagct ttaaacttac ctagacggcg gacgcagttc aggaggcacc acaggcggga
     9781 ggcggcagaa cgcgactcaa ccggcgtgga tggcggcctc aggtagggcg gcgggcgcgt
     9841 gaaggagaga tgcgagcccc tcgaagcttc agctgtgttc tggcggcaaa cccgttgcga
     9901 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
     9961 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
    10021 cccgttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct
    10081 gcccactgac gggcaccgga gcgatcgcag atccgtcgac tctagcttgg ctgcaggtcg
    10141 tcgaaattct acc
//
