LOCUS       sequence-245801-        8313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_MSP3.5-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3477..3494
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3546..3601
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3600..3619)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5837..5855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5923..6027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6028..6096,6097..6888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6246..6265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7059..7647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7548..7567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7801..7818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7935..7956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7971..7976,7977..7994,7995..8001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8009..8025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8014..8036
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8033..8049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8033..8049
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 atgacgccaa gctgatcgac aacagcccca acaacggcta catcaaccag aagaagtaca
     1261 acctgcggaa caacatgccc atcaagaata tcgcccctca ggacgcccag aacaacctgg
     1321 aagccaagaa cagcaacgag aacaacgcct acgagggcaa caccgacgtg ctggaaaacg
     1381 tgcacaacgc cgccaacatc ctgaacaagc agaagtacga ggaattcggc gacagcaaca
     1441 ccgccagcaa cttcgccaag agcttcaagc acacccacac caacgcccag ctgctgatcc
     1501 ccatggaccc tcagctgcag cagcccacca gcagcgaccc tcacaacagc atgcacaccg
     1561 tgctgggcaa cgacgccgag gaaggcaaag gacctggcac cgcccaggaa cccatcaatc
     1621 agcccgagag cagaaagacc ctggaagagg aagagaagga caagaaggtg ctgagcgagg
     1681 aacagagccg gaccaaggcc cctgagctgc ctcagagcag cgtggaaaca gagggcgagc
     1741 acagcaccac cggcatcgag cagcctggca aggatctgag cacaggcgtg gacagccctc
     1801 agggcatcgt gccaccacag gtggtcctgc ctggcagcga tcctagcgag agaaccgtgc
     1861 ccagcaaaga ggtggacgtc gaccctccac ctagcgagcc tagcaccctg gctagagtgc
     1921 cccaggaaaa gagcgccagc agcggcccag agaagaaaga ggacatccag agcaagagcg
     1981 accccaactc caacgtgtac cagaaagagc agggcctggg cctgaccacc gacatcagcg
     2041 tgcagaccga cagcgctcag gaactgaaag aacagcggga ccagagccac caggaacgga
     2101 agggcgagga acggcccgag acacaggatc tgggcaccct gagcaccaac gaggatccaa
     2161 gcggcgagcc cctggaacag cagagaggcg acggcaagat caaggacgac gccgccgagg
     2221 acgagaactc cgccaccgtg gaaaagtcca gagtgtccga gggcctgagc aacagcgacg
     2281 aggaagccca ggaccatctg cccagccctc cacaggaaga agaacagaga cagcagatgc
     2341 agcagctgag ccagcaggaa gagagatccg aggaagctca gaacaaagag gaaaacaacg
     2401 tcctccacac cgtgtccatc ctgcccggcg agagcgccaa tatcctggat aagaacgagg
     2461 aagtggtcga ggaaaaagag gaacacatcg aggaagatga agaagaggag gaagagggcc
     2521 ggcagaaaga agagatgaac gtctaccagg aggaagccga gacaaacaac gaagaagaga
     2581 aagaggaaga ttacgaagag gacgagatcg aggaagtcgt gggcggaggc aagaagaaca
     2641 acgacgccag catcggcacc gaagtgcgga agatcctgag cggcgagtac gacgacatga
     2701 acaagttcaa gaccatcacc gagatcagca tcaagagcct gatcaactcc ttcgacggca
     2761 acaacaaagt gacaaacatc ctcaacgacc tggtcaagga catggcccac ttctacctga
     2821 agtctggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     2881 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     2941 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     3001 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     3061 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     3121 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     3181 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     3241 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     3301 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     3361 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     3421 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     3481 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     3541 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     3601 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     3661 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     3721 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     3781 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     3841 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     3901 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     3961 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     4021 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     4081 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     4141 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     4201 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     4261 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     4321 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     4381 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     4441 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     4501 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     4561 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     4621 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     4681 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     4741 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     4801 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     4861 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     4921 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     4981 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     5041 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     5101 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     5161 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     5221 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     5281 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     5341 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     5401 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     5461 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     5521 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     5581 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     5641 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     5701 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     5761 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     5821 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5881 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5941 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6001 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6061 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6121 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6181 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6241 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6301 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6361 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6421 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6481 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6541 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     6601 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6661 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6721 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6781 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6841 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6901 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6961 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7021 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7081 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7141 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7201 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7261 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7381 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7441 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7501 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7561 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7621 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7681 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7741 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7801 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7861 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7921 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7981 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8041 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     8101 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     8161 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     8221 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     8281 acatttatat tggctcatgt ccaatatgac cgc
//
