LOCUS       sequence-245802-        3465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NAC domain (1-215) of TtNAM-B1 in the Gateway-compatible pCR8 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
  TITLE     Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC 
            domain are required for protein binding and when mutated lead to 
            delayed peduncle and flag leaf senescence.
  JOURNAL   BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 
            10.1186/s12870-019-2022-5.
  PUBMED    31533618
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2019-09-18"; volume: "19(1):407. doi"; pages: " 
            10.1186/s12870-019-2022-5"
COMMENT     Sequence Label: pCR8_TtNAM-B1(1-215)
FEATURES             Location/Qualifiers
     source          1..3465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(54..140)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     189..211
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     203..220
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     204..220
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    236..335
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1011..1110)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1127..1146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1128..1146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1151..1167)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1151..1167)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1598..2389
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2482..3070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2971..2990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3224..3241
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3400..3427)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
       61 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
      121 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
      181 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
      241 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
      301 ttttataatg ccaactttgt acaaaaaagc aggctccgaa ttcgccctta tgggcagctc
      361 cgactcatct tccggctcgg cgcaaaaagc aacgcggtat caccatcagc atcagccgcc
      421 gcctccgcag cggggctcgg cgccggagct cccgccgggc ttccggttcc acccgacgga
      481 cgaggagctg gtggtgcact acctcaagaa gaaggccgac aaggcgccgc tccccgtcaa
      541 catcatcgcc gaggtggatc tctacaagtt cgacccatgg gagctccccg agaaggcgac
      601 catcggggag caggagtggt acttcttcag cccgcgcgac cgcaagtacc ccaacggcgc
      661 gcggccgaac cgggcggcga cgtcggggta ctggaaggcc accggcacgg acaagcctat
      721 cctggcctcg gggacggggt gcggcctggt ccgggagaag ctcggcgtca agaaggcgct
      781 cgtgttctac cgcgggaagc cgcccaaggg cctcaaaacc aactggatca tgcacgagta
      841 ccgcctcacc gacgcatctg gctccaccac cgccaccaac cgaccgccgc cggtgaccgg
      901 cgggagcagg gctgctgcct ctctcaggtt ggacgactgg gtgctgtgcc gcatctacaa
      961 gaagatcaac aaggccgcgg ccggcgatca gcagtgaaag ggcgaattcg acccagcttt
     1021 cttgtacaaa gttggcatta taaaaaataa ttgctcatca atttgttgca acgaacaggt
     1081 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     1141 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     1201 ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa
     1261 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     1321 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     1381 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1441 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     1501 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1561 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     1621 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1681 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     1741 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1801 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1861 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1921 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1981 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     2041 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     2101 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     2161 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     2221 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     2281 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     2341 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca
     2401 cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     2461 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     2521 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     2581 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     2641 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     2701 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2761 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2821 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2881 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2941 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3001 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3061 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3121 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3181 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3241 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     3301 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     3361 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     3421 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcct
//
