LOCUS       sequence-245803-        4044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TaNAM-A1 CDS in the Gateway-compatible pCR8 vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Harrington SA, Overend LE, Cobo N, Borrill P, Uauy C
  TITLE     Conserved residues in the wheat (Triticum aestivum) NAM-A1 NAC 
            domain are required for protein binding and when mutated lead to 
            delayed peduncle and flag leaf senescence.
  JOURNAL   BMC Plant Biol. 2019 Sep 18;19(1):407. doi: 
            10.1186/s12870-019-2022-5.
  PUBMED    31533618
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2019-09-18"; volume: "19(1):407. doi"; pages: " 
            10.1186/s12870-019-2022-5"
COMMENT     Sequence Label: pCR8_TaNAM-A1
FEATURES             Location/Qualifiers
     source          1..4044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1355..1454)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1471..1490)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1472..1490)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1495..1511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1495..1511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1942..2733
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2826..3414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3315..3334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3568..3585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3744..3771)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3863..3949)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3998..4020
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4012..4029
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4013..4029
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttatgagg
      121 tccatgggca gctccgactc atcctccggc tcggcgcaaa aagcagcgcg gcatcagcat
      181 gagccgccgc ctccgcggca gcggggctcg gcgccggagc tcccaccggg cttccggttc
      241 cacccgacgg acgaggagct ggtcgtgcac tacctcaaga agaaggccgc caaggtgccg
      301 ctccccgtca ccatcatcgc cgaggtggat ctctacaagt tcgacccatg ggagctcccc
      361 gagaaggcga ccttcgggga gcaggagtgg tacttcttca gcccgcgcga ccgcaagtac
      421 cccaacggcg cgcggccgaa ccgggcggcg acgtcgggct actggaaggc caccggcacg
      481 gacaaaccta tcctggcctc ggggacgggg tgcggcctgg tccgggagaa gctcggcgtc
      541 aagaaggcgc tcgtcttcta ccgcgggaag ccgcccaagg gcctcaaaac caactggatc
      601 atgcacgagt accgcctcac cgacgcgtct ggctccacca ccaccagccg gccgccgccg
      661 cctgtgaccg gcgggagccg ggctgcagcc tctctgaggt tggacgactg ggtgctgtgc
      721 cgcatctaca agaagatcaa caaggccgcg gccggagatc agcagaggag cacggagtgc
      781 gaggactccg tggaggacgc ggtcaccgcg tacccgctct atgccacggc gggcatggcc
      841 ggtgcaggtg cgcatggcag caactacgct tcaccttcac tgctccatca tcaggacagc
      901 catttcctgg agggcctgtt cacagcagac gacgccggcc tctcggcggg cgccacctcg
      961 ctgagccacc tggccgcggc ggcgagggcg agcccggctc cgaccaaaca gtttctcgcc
     1021 ccgtcgtctt caaccccgtt caactggctc gatgcgtcac ccgccggcat cctgccacag
     1081 gcaaggaatt tccctgggtt taacaggagc agaaacgtcg gcaatatgtc gctgtcatcg
     1141 acggccgaca tggctggcgc ggccggcaat gcggtgaacg ccatgtccgc atttatgaat
     1201 cctctccccg tgcaagacgg gacgtaccat caacaccatg tcatcctcgg cgccccactg
     1261 gcgccagagg ctaccacagg cggcgccacc tctggtttcc agcatcccgt ccaagtatcc
     1321 ggcgtgaact ggaatccctg aaagggcgaa ttcgacccag ctttcttgta caaagttggc
     1381 attataaaaa ataattgctc atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1441 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1501 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1561 aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg
     1621 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     1681 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     1741 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     1801 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1861 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1921 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1981 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2041 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     2101 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2161 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2221 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2281 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2341 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2401 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2461 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2521 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2581 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2641 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     2701 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc
     2761 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2821 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2881 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2941 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3001 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3061 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3181 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3601 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3661 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     3721 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     3781 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     3841 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     3901 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     3961 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4021 acggccagtc ttaagctcgg gccc
//
