LOCUS       sequence-24582-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pShuttle-CMV converted Gateway Destination vector for AdEasy 
            adenoviral vector system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZYshuttle
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..209
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(741..760)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cggggggcnt tagcgagctg gttngtgaac cgtcagatcc gctagagatc tggtaccgtc
       61 gacgcggccg ctgaattcat taatacaagt ttgtacaaaa aagctgaacg agaaacgtaa
      121 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
      181 tgtaaaacac aacatatcca gtcactatga atcaactact tagatggtat tagtgacctg
      241 tagtcgaccg acagccttcc aaatgttctt cgggtgatgc tgccaactta gtcgaccgac
      301 agccttccaa atgttcttct caaacggaat cgtcgtatcc agcctactcg ctattgtcct
      361 caatgccgta ttaaatcata aaaagaaata agaaaaagag gtgcgagcct cttttttgtg
      421 tgacaaaata aaaacatcta cctattcata tacgctagtg tcatagtcct gaaaatcatc
      481 tgcatcaaga acaatttcac aactcttata cttttctctt acaagtcgtt cggcttcatc
      541 tggattttca gcctctatac ttactaaacg tgataaagtt tctgtaattt ctactgtatc
      601 gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt
      661 taatggcgtt tttgatgtca ttttnccggg gnggctgaga tcagccactt cttccccgtg
      721 nacggagacn ggccnctggc catatcggtg gtcatcatgc gccagcttca tcccgattgc
      781 acaccggtaa ggtccgggag acttatctga cgcagacgtg ccngccgggg acnctcc
//
