LOCUS       sequence-24583-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJG4-5 converted Gateway Destination vector for yeast two-hybrid 
            prey vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZY45
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        113..554
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    113..230
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     525..548
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             578..598
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caatgntgat tcgaattaat tcgagctcgg taccccatta tcttagccta aaaaaacctt
       61 ctctttggaa ctttcagtaa tacgcttaac tgctcattgc tatattgaag tacggattag
      121 aagccgccga gcgggtgaca gccctccgaa ggaagactct cctccgtgcg tcctcgtctt
      181 caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag
      241 attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca
      301 caaaccttca aatgaacgaa tcaaattaac aaccatagga tgataatgcg attagttttt
      361 tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata
      421 tataaatgca aaaactgcat aaccacttta actaatactt tcaacatttt cggtttgtat
      481 tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata cctctatact
      541 ttaacgtcaa ggaggaatta agcttatggg tgctcctcca aaaaagaaga gaaaggtagc
      601 tggtatcaat aaagatatcg aggagtgcaa tgccatcatt gagcagttta tcgactacct
      661 gccccgngna acaggagatg ccgatggaat gggcggatca ggcgattaac gtggtgccgg
      721 gcatgacgcc gaaaaccatt cttcacgccg ggccgccgat ccagcctgac tggctgaatc
      781 gatggtttca tgaattgaag cggaggtaac gaaccgcctc tgngagg
//
