LOCUS       sequence-245847-        1217 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pET28B-PU.1-ETS-Loop
FEATURES             Location/Qualifiers
     source          1..1217
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             367..384
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             391..408
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             433..450
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(503..521)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      517..564
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      601..1029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(688..707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     898..919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gap             1192..1203
                     /estimated_length=12
ORIGIN
        1 annntcccnc tagaataatt ttgtttaact ttaagaagga gatataccat gggttctaag
       61 aaaaagatcc gtctgtacca atttctgtta gacctgttgc gttcaggaga catgaaggat
      121 tcaatctggt gggtagataa ggataaaggg acatttcagt tcagcagtaa gcacaaggag
      181 gctctggcgc accgttgggg gaaacgcaaa aacaaaccaa aaatgacgta ccaaaagatg
      241 gcgcgtgcgc tgcgtaacta cggaaagact ggcgaagtta agaaggtgaa gaaaaaattg
      301 acgtatcagt tttctggaga agtgttaggg cgtggcgggt tggctgaacg ccgtctgcct
      361 ccacatttag tcccgcgcgg gtctcgttcg catcaccatc atcaccacta aaagcttgcg
      421 gccgcactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      481 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      541 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      961 nanggntttt gncgatttcg gcctattggn naaaaaatga gctgatttaa caaaaatttn
     1021 acgcgaattt tannaatatt aacgcttann nttagtgnac ttttcgggga ntgtgcgnnn
     1081 ccctatttnt tantttttcn aatacntnan natgnatcgc tcannnnntt nnnnaanntc
     1141 ntcnngcatn aannaacnnc nntnnttcnn ntcnnnntnt nnnnnnnnnt tnnnnnnnnn
     1201 nnntnnnnnt nnngnna
//
