LOCUS       sequence-24585-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pPIC3.5K converted Gateway Destionation vector for multiple copy 
            integration of gene of interest into Pichia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZYpci
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(224..243)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gcctgnggcg ccggtnagcc gacctgcagg gggggggggg aaagccacgt tgtgtctcaa
       61 aatcnctgnt gttacattgc acaagataaa aatatatcat cntgaacaat aaaactgtct
      121 gcttacataa acagtaatac aaggggngtt atgagccata ttcaacggga aacgtcttgc
      181 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
      241 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
      301 nanttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacnga tgagatggtc
      361 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
      421 cctgatgatg catggntact caccactgcg atccccggga aaacagcatt ccaggtatta
      481 naanaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
      541 ttgcattcga ttcctgnttg naattgtcct tttaacagcg atcgcgtatt tcgtctcgct
      601 caggcgcaat cncgaatgaa taacggtttg gttgatgcca gtgattttga tgaccagcgt
      661 aatggctggc ctgttgaaca actctggaaa gaaatgcata agcttttgcc attctcaccg
      721 gantcagtcg tcactcatgg tgattctcac ttgataaccc tatttttgac gagggaaata
      781 ataggtgtnt gntgttgaca tcggaaccca ancgnncngg tctgctctng nangc
//
