LOCUS       sequence-245903-        2170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for L3S2P55 (256x-strength 
            synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2170)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T16m
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..45
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(49..92)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    95..116
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    208..228
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(234..251)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(405..993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(485..504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1164..1955,1956..2024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1787..1806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2025..2129)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa
       61 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta
      121 gtgggtctca aggtacggtc ggtaccaaag acgaacaata agacgctgaa aagcgtcttt
      181 tttcgttttg gtcccggctt agagacctac tagtagcggc cgctgcaggc ttcctcgctc
      241 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      301 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
      421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      601 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      841 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1141 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1201 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     1261 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg
     1321 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1381 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1441 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1501 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1561 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1621 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1681 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1741 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     1801 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1861 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1921 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1981 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2041 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2101 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     2161 taagaaacca
//
