LOCUS       sequence-245907-        8460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of yeast codon-optimized eGFP and 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW yoeGFP-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(835..837,838..1548)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             1555..1608
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1624..1633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1630..2340
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1775..1793)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2043..2062
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2350..2374)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2436..2455)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2437..2455)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2603..2790
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2658..2682)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2802..2819)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2802..2818)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2811..2833)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3254..4600
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4711..5514)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5515..5730)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5755..5773)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5841..5945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5946..6014,6015..6806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6164..6183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6977..7565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7466..7485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7719..7736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7853..7874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7889..7894,7895..7912,7913..7919)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7927..7943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7932..7954
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7951..7967
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7951..7967
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(7997..8452)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8134..8155)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8346..8365
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc caccatgtca
      841 aaaggcgagg aactattcac aggtgtcgta ccaatactgg tagaattgga tggcgacgta
      901 aacgggcata agttttctgt gagtggtgag ggtgaagggg acgcaactta tggcaaactt
      961 acacttaagt tcatctgcac cacagggaag ctgcctgtcc catggccaac tctagttaca
     1021 actctcactt acggagtaca atgtttttct cgatacccag accacatgaa acaacatgac
     1081 tttttcaaat ctgccatgcc tgagggatat gtgcaagaga gaactatttt ctttaaagat
     1141 gatggtaatt acaaaactcg tgctgaagtg aagtttgaag gagatacact tgttaataga
     1201 attgagttaa agggtattga ctttaaggaa gatggtaata tcttaggtca caaattggaa
     1261 tacaactaca attcacataa cgtttacatc atggcagata aacagaaaaa cggcataaaa
     1321 gttaatttca aaattagaca taatatcgaa gatggttccg ttcaactcgc tgatcattat
     1381 caacaaaata caccaatcgg agatggacct gttctattgc cagacaacca ttacttatct
     1441 acacagagcg ccttgtcaaa agatcctaat gaaaagagag atcacatggt tttgttagaa
     1501 tttgtcaccg ctgcgggtat aacgttgggc atggatgaat tatacaagtc cggagagggc
     1561 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctgg ggatccaccg
     1621 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1681 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1741 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1801 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1861 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1921 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1981 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2041 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2101 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2161 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2221 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     2281 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     2341 agcggccgca cccagctttc ttgtacaaag tggtgtagtt aattcatgat ggccgctgca
     2401 ggtcgacctc gagggggggc ccggtaccca attcgcccta tagtgagtcg tattacgcgc
     2461 ggatccagct ttggacttct tcgccagagg tttggtcaag tctccaatca aggttgtcgg
     2521 cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata
     2581 cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata
     2641 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     2701 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagcatga
     2761 ggtcgctctt attgaccaca cctctaccgg catgccaatt cactggccgt cgttttacaa
     2821 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     2881 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2941 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3001 tcacaccgca taatcggatc gtacttgtta cccatcattg aattttgaac atccgaacct
     3061 ggaagttttc cctgaaacag atagtatatt tgaacctgta taataatata tagtctagcg
     3121 ctttacggaa gacaatgtat gtatttcggt tcctggagaa actattgcat ctattgcata
     3181 ggtaatcttg cacgtcgcat ccccggttca ttttctgcgt ttccatcttg cacttcaata
     3241 gcatatcttt gttaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
     3301 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
     3361 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
     3421 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     3481 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
     3541 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
     3601 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
     3661 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
     3721 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
     3781 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
     3841 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     3901 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     3961 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     4021 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     4081 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     4141 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     4201 ttttcaaaag cgctctgaag ttcctatact ttctagctag agaataggaa cttcggaata
     4261 ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca
     4321 tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg
     4381 agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt
     4441 aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta
     4501 tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag
     4561 tctcatcctt caatgctatc atttcctttg atattggatc gatccgatga taagctgtca
     4621 aacatgagaa ttgggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag
     4681 tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata
     4741 tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc
     4801 aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt
     4861 tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat
     4921 caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac
     4981 aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag
     5041 ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc
     5101 ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt
     5161 aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt
     5221 accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc
     5281 ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt
     5341 tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg
     5401 aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc
     5461 agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta
     5521 tcttcgtttc ctgcatgttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca
     5581 tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg
     5641 ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc gaaatcaaaa
     5701 aaaagaataa aaaaaaaatg atgaattgaa aagctaattc ttgaagacga aagggcctcg
     5761 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5821 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5881 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5941 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6001 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6061 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6121 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6181 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6241 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6301 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6361 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6421 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6481 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6541 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6601 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6661 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6721 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6781 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6841 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6901 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6961 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7021 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7081 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7141 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7201 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7261 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7321 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7381 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7441 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7501 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7561 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7621 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7681 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7741 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7801 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7861 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7921 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7981 agcttaccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     8041 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     8101 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     8161 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     8221 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     8281 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     8341 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     8401 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
//
