LOCUS       sequence-245931-       12119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR E709_T710delinsD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12119)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-E709_T710delinsD
FEATURES             Location/Qualifiers
     source          1..12119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3448..3519,3520..5382,5383..5451,5452..7077)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKDEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPK
                     ANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQY
                     LLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAE
                     GGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGE
                     RLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLP
                     SPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVAC
                     IDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNP
                     VYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISL
                     DNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(7079..7103)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7111..7129
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7154..7173)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7155..7173)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7179..7199)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7253..7294
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7315..7882
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7332..7884
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7476..7493)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7703..7722
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7896..8495
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7896..7915)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8392..8412
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8509..9097
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8562..8582)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8980..8991)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9172..9405
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9524..9542)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9610..9714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9715..9783,9784..10575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10746..11334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11235..11254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11488..11505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11745..11766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11781..11786,11787..11804,11805..11811)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11819..11835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11824..11846
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11843..11859
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11843..11859
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11909..11928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cgaccctccg ggacggccgg ggcagcgctc
     3481 ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc
     3541 caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt tctcagcctc
     3601 cagaggatgt tcaataactg tgaggtggtc cttgggaatt tggaaattac ctatgtgcag
     3661 aggaattatg atctttcctt cttaaagacc atccaggagg tggctggtta tgtcctcatt
     3721 gccctcaaca cagtggagcg aattcctttg gaaaacctgc agatcatcag aggaaatatg
     3781 tactacgaaa attcctatgc cttagcagtc ttatctaact atgatgcaaa taaaaccgga
     3841 ctgaaggagc tgcccatgag aaatttacag gaaatcctgc atggcgccgt gcggttcagc
     3901 aacaaccctg ccctgtgcaa cgtggagagc atccagtggc gggacatagt cagcagtgac
     3961 tttctcagca acatgtcgat ggacttccag aaccacctgg gcagctgcca aaagtgtgat
     4021 ccaagctgtc ccaatgggag ctgctggggt gcaggagagg agaactgcca gaaactgacc
     4081 aaaatcatct gtgcccagca gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc
     4141 tgccacaacc agtgtgctgc aggctgcaca ggcccccggg agagcgactg cctggtctgc
     4201 cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc ccccactcat gctctacaac
     4261 cccaccacgt accagatgga tgtgaacccc gagggcaaat acagctttgg tgccacctgc
     4321 gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt
     4381 ggggccgaca gctatgagat ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg
     4441 ccttgccgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata
     4501 aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc
     4561 ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa
     4621 ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct
     4681 gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag
     4741 caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc
     4801 tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat
     4861 gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata
     4921 agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc
     4981 cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga
     5041 ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag
     5101 aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc
     5161 acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc
     5221 gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca
     5281 gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca
     5341 ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg
     5401 ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gcgaaggcgc
     5461 cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg agagggagct tgtggagcct
     5521 cttacaccca gtggagaagc tcccaaccaa gctctcttga ggatcttgaa ggatgaattc
     5581 aaaaagatca aagtgctggg ctccggtgcg ttcggcacgg tgtataaggg actctggatc
     5641 ccagaaggtg agaaagttaa aattcccgtc gctatcaagg aattaagaga agcaacatct
     5701 ccgaaagcca acaaggaaat cctcgatgaa gcctacgtga tggccagcgt ggacaacccc
     5761 cacgtgtgcc gcctgctggg catctgcctc acctccaccg tgcagctcat cacgcagctc
     5821 atgcccttcg gctgcctcct ggactatgtc cgggaacaca aagacaatat tggctcccag
     5881 tacctgctca actggtgtgt gcagatcgca aagggcatga actacttgga ggaccgtcgc
     5941 ttggtgcacc gcgacctggc agccaggaac gtactggtga aaacaccgca gcatgtcaag
     6001 atcacagatt ttgggctggc caaactgctg ggtgcggaag agaaagaata ccatgcagaa
     6061 ggaggcaaag tgcctatcaa gtggatggca ttggaatcaa ttttacacag aatctatacc
     6121 caccagagtg atgtctggag ctacggggtg accgtttggg agttgatgac ctttggatcc
     6181 aagccatatg acggaatccc tgccagcgag atctcctcca tcctggagaa aggagaacgc
     6241 ctccctcagc cacccatatg taccatcgat gtctacatga tcatggtcaa gtgctggatg
     6301 atagacgcag atagtcgccc aaagttccgt gagttgatca tcgaattctc caaaatggcc
     6361 cgagaccccc agcgctacct tgtcattcag ggggatgaaa gaatgcattt gccaagtcct
     6421 acagactcca acttctaccg tgccctgatg gatgaagaag acatggacga cgtggtggat
     6481 gccgacgagt acctcatccc acagcagggc ttcttcagca gcccctccac gtcacggact
     6541 cccctcctga gctctctgag tgcaaccagc aacaattcca ccgtggcttg cattgataga
     6601 aatgggctgc aaagctgtcc catcaaggaa gacagcttct tgcagcgata cagctcagac
     6661 cccacaggcg ccttgactga ggacagcata gacgacacct tcctcccagt gcctgaatac
     6721 ataaaccagt ccgttcccaa aaggcccgct ggctctgtgc agaatcctgt ctatcacaat
     6781 cagcctctga accccgcgcc cagcagagac ccacactacc aggaccccca cagcactgca
     6841 gtgggcaacc ccgagtatct caacactgtc cagcccacct gtgtcaacag cacattcgac
     6901 agccctgccc actgggccca gaaaggcagc caccaaatta gcctggacaa ccctgactac
     6961 cagcaggact tctttcccaa ggaagccaag ccaaatggca tctttaaggg ctccacagct
     7021 gaaaatgcag aatacctaag ggtcgcgcca caaagcagtg aatttattgg agcatagaac
     7081 ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaaggt taaagatgtt
     7141 aaagaatgag tgaccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt
     7201 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     7261 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc
     7321 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     7381 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     7441 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     7501 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     7561 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     7621 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     7681 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     7741 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     7801 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     7861 cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc
     7921 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     7981 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     8041 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     8101 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     8161 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     8221 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     8281 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     8341 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     8401 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     8461 gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg
     8521 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8581 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8641 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8701 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8761 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8821 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8881 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8941 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     9001 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     9061 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     9121 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     9181 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     9241 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9301 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     9361 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     9421 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     9481 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     9541 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     9601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     9841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     9901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     9961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    11041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    11101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    11161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    11221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    11281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    11341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    11401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
    11581 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
    11641 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
    11701 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    11761 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    11821 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    11881 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    11941 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    12001 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    12061 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
