LOCUS       sequence-245933-       12121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR S380Pfs*16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-S380Pfs*16
FEATURES             Location/Qualifiers
     source          1..12121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7081..7105)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7113..7131
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7156..7175)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7157..7175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7181..7201)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7255..7296
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7317..7884
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7334..7886
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7478..7495)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7705..7724
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7898..8497
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7898..7917)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8394..8414
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8511..9099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8564..8584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8982..8993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9174..9407
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9526..9544)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9612..9716
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9717..9785,9786..10577)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10748..11336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11237..11256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11490..11507
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11747..11768
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11783..11788,11789..11806,11807..11813)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11821..11837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11826..11848
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11845..11861
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11845..11861
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11911..11930
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cgaccctccg ggacggccgg ggcagcgctc
     3481 ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc
     3541 caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt tctcagcctc
     3601 cagaggatgt tcaataactg tgaggtggtc cttgggaatt tggaaattac ctatgtgcag
     3661 aggaattatg atctttcctt cttaaagacc atccaggagg tggctggtta tgtcctcatt
     3721 gccctcaaca cagtggagcg aattcctttg gaaaacctgc agatcatcag aggaaatatg
     3781 tactacgaaa attcctatgc cttagcagtc ttatctaact atgatgcaaa taaaaccgga
     3841 ctgaaggagc tgcccatgag aaatttacag gaaatcctgc atggcgccgt gcggttcagc
     3901 aacaaccctg ccctgtgcaa cgtggagagc atccagtggc gggacatagt cagcagtgac
     3961 tttctcagca acatgtcgat ggacttccag aaccacctgg gcagctgcca aaagtgtgat
     4021 ccaagctgtc ccaatgggag ctgctggggt gcaggagagg agaactgcca gaaactgacc
     4081 aaaatcatct gtgcccagca gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc
     4141 tgccacaacc agtgtgctgc aggctgcaca ggcccccggg agagcgactg cctggtctgc
     4201 cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc ccccactcat gctctacaac
     4261 cccaccacgt accagatgga tgtgaacccc gagggcaaat acagctttgg tgccacctgc
     4321 gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt
     4381 ggggccgaca gctatgagat ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg
     4441 ccttgccgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata
     4501 aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc
     4561 ctgccggtgg catttagggg tgacccttca cacatactcc tcctctggat ccacaggaac
     4621 tggatattct gaaaaccgta aaggaaatca cagggttttt gctgattcag gcttggcctg
     4681 aaaacaggac ggacctccat gcctttgaga acctagaaat catacgcggc aggaccaagc
     4741 aacatggtca gttttctctt gcagtcgtca gcctgaacat aacatccttg ggattacgct
     4801 ccctcaagga gataagtgat ggagatgtga taatttcagg aaacaaaaat ttgtgctatg
     4861 caaatacaat aaactggaaa aaactgtttg ggacctccgg tcagaaaacc aaaattataa
     4921 gcaacagagg tgaaaacagc tgcaaggcca caggccaggt ctgccatgcc ttgtgctccc
     4981 ccgagggctg ctggggcccg gagcccaggg actgcgtctc ttgccggaat gtcagccgag
     5041 gcagggaatg cgtggacaag tgcaaccttc tggagggtga gccaagggag tttgtggaga
     5101 actctgagtg catacagtgc cacccagagt gcctgcctca ggccatgaac atcacctgca
     5161 caggacgggg accagacaac tgtatccagt gtgcccacta cattgacggc ccccactgcg
     5221 tcaagacctg cccggcagga gtcatgggag aaaacaacac cctggtctgg aagtacgcag
     5281 acgccggcca tgtgtgccac ctgtgccatc caaactgcac ctacggatgc actgggccag
     5341 gtcttgaagg ctgtccaacg aatgggccta agatcccgtc catcgccact gggatggtgg
     5401 gggccctcct cttgctgctg gtggtggccc tggggatcgg cctcttcatg cgaaggcgcc
     5461 acatcgttcg gaagcgcacg ctgcggaggc tgctgcagga gagggagctt gtggagcctc
     5521 ttacacccag tggagaagct cccaaccaag ctctcttgag gatcttgaag gaaactgaat
     5581 tcaaaaagat caaagtgctg ggctccggtg cgttcggcac ggtgtataag ggactctgga
     5641 tcccagaagg tgagaaagtt aaaattcccg tcgctatcaa ggaattaaga gaagcaacat
     5701 ctccgaaagc caacaaggaa atcctcgatg aagcctacgt gatggccagc gtggacaacc
     5761 cccacgtgtg ccgcctgctg ggcatctgcc tcacctccac cgtgcagctc atcacgcagc
     5821 tcatgccctt cggctgcctc ctggactatg tccgggaaca caaagacaat attggctccc
     5881 agtacctgct caactggtgt gtgcagatcg caaagggcat gaactacttg gaggaccgtc
     5941 gcttggtgca ccgcgacctg gcagccagga acgtactggt gaaaacaccg cagcatgtca
     6001 agatcacaga ttttgggctg gccaaactgc tgggtgcgga agagaaagaa taccatgcag
     6061 aaggaggcaa agtgcctatc aagtggatgg cattggaatc aattttacac agaatctata
     6121 cccaccagag tgatgtctgg agctacgggg tgaccgtttg ggagttgatg acctttggat
     6181 ccaagccata tgacggaatc cctgccagcg agatctcctc catcctggag aaaggagaac
     6241 gcctccctca gccacccata tgtaccatcg atgtctacat gatcatggtc aagtgctgga
     6301 tgatagacgc agatagtcgc ccaaagttcc gtgagttgat catcgaattc tccaaaatgg
     6361 cccgagaccc ccagcgctac cttgtcattc agggggatga aagaatgcat ttgccaagtc
     6421 ctacagactc caacttctac cgtgccctga tggatgaaga agacatggac gacgtggtgg
     6481 atgccgacga gtacctcatc ccacagcagg gcttcttcag cagcccctcc acgtcacgga
     6541 ctcccctcct gagctctctg agtgcaacca gcaacaattc caccgtggct tgcattgata
     6601 gaaatgggct gcaaagctgt cccatcaagg aagacagctt cttgcagcga tacagctcag
     6661 accccacagg cgccttgact gaggacagca tagacgacac cttcctccca gtgcctgaat
     6721 acataaacca gtccgttccc aaaaggcccg ctggctctgt gcagaatcct gtctatcaca
     6781 atcagcctct gaaccccgcg cccagcagag acccacacta ccaggacccc cacagcactg
     6841 cagtgggcaa ccccgagtat ctcaacactg tccagcccac ctgtgtcaac agcacattcg
     6901 acagccctgc ccactgggcc cagaaaggca gccaccaaat tagcctggac aaccctgact
     6961 accagcagga cttctttccc aaggaagcca agccaaatgg catctttaag ggctccacag
     7021 ctgaaaatgc agaataccta agggtcgcgc cacaaagcag tgaatttatt ggagcataga
     7081 acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag gttaagtgaa
     7141 gtgttgttta ttgaacccta tagtgagtcg tattaccacc caacttttct atacaaagtg
     7201 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     7261 cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta atgagatccc
     7321 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     7381 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     7441 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     7501 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     7561 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     7621 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     7681 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     7741 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     7801 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     7861 ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca agcccacggt
     7921 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     7981 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     8041 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     8101 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     8161 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     8221 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     8281 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     8341 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     8401 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     8461 gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct aatcaacctc
     8521 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     8581 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     8641 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     8701 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     8761 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     8821 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     8881 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     8941 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     9001 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     9061 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat
     9121 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     9181 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     9241 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9301 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9361 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9421 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     9481 cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
     9541 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     9601 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9661 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9721 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9781 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9841 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9901 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     9961 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10021 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10081 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10141 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10201 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10261 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10321 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10381 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10441 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10501 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10561 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10621 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10681 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10741 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10801 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10861 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    10921 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10981 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11041 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11101 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11161 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11221 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11281 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    11341 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    11401 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11461 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11521 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
    11581 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    11641 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
    11701 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11761 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11821 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
    11881 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    11941 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    12001 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    12061 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    12121 t
//
