LOCUS       sequence-245934-        8628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pYPQ255
FEATURES             Location/Qualifiers
     source          1..8628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..87)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     136..158
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     150..167
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     151..167
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    183..282
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             298..984
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             1039..1086
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             1093..1113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1138..2574,2764..5427)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          2575..2763
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             5428..5475
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5497..5745
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5758..5778
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5800..6052
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    6097..6220
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6237..6256)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6238..6256)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6261..6277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6261..6277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6708..7499
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7592..8180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8081..8100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8334..8351
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8510..8537)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
       61 ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc
      121 ttgcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc
      181 cccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc
      241 aatgcttttt tataatgcca actttgtaca aaaaagcagg ctccgaattc gcccttcatg
      301 agttctgaaa caggtccagt ggctgtcgat ccgactctga ggaggcgcat cgaaccacac
      361 gaatttgagg ttttttttga tccaagagag cttcgcaagg aaacttgtct cctgtatgag
      421 ataaattggg gaggaagaca tagtatctgg agacacacaa gccagaacac caataagcat
      481 gtcgaagtta acttcatcga aaaatttacg accgaacgct acttttgtcc taatacacgc
      541 tgcagtatca cttggttcct ttcgtggtca ccttgcggtg aatgttccag agcaattacg
      601 gagtttctct cgagatatcc acacgttaca cttttcattt acatagctcg gctttatcat
      661 cacgcagatc cgagaaatcg ccaggggctg cgcgatctga tttcgtccgg tgtcaccatt
      721 caaattatga ctgagcagga atctggatac tgctggcgca acttcgtgaa ctacagtccg
      781 tcgaatgaag cgcactggcc gagatatccc catctctggg tgcgcttgta tgttttggag
      841 ctttattgta tcatcttggg attgccacct tgtcttaata tattgcgccg gaaacaaccg
      901 cagctcactt ttttcaccat tgctctgcag tcttgtcact accaacgcct ccccccgcac
      961 atcctgtggg ccacaggcct taaatcaggt tctgagaccc ctggcacaag tgagtcagca
     1021 acacccgagt ccaccatgga ttacaaggat gatgatgata aggattacaa ggatgatgat
     1081 gataagatgg ctccaaagaa gaagagaaag gttggaatcc acggagttcc agctgctgat
     1141 aagaagtact ctatcggact tgccatcgga accaactctg ttggatgggc tgttatcacc
     1201 gatgagtaca aggttccatc taagaagttc aaggttcttg gaaacaccga tagacactct
     1261 atcaagaaga accttatcgg tgctcttctt ttcgattctg gagagaccgc tgaggctacc
     1321 agattgaaga gaaccgctag aagaagatac accagaagaa agaacagaat ctgctacctt
     1381 caggaaatct tctctaacga gatggctaag gttgatgatt ctttcttcca cagacttgag
     1441 gagtctttcc ttgttgagga ggataagaag cacgagagac acccaatctt cggaaacatc
     1501 gttgatgagg ttgcttacca cgagaagtac ccaaccatct accaccttag aaagaagttg
     1561 gttgattcta ccgataaggc tgatcttaga cttatctacc ttgctcttgc tcacatgatc
     1621 aagttcagag gacacttcct tatcgaggga gaccttaacc cagataactc tgatgttgat
     1681 aagttgttca tccagcttgt tcagacctac aaccagcttt tcgaggagaa cccaatcaac
     1741 gcttctggag ttgatgctaa ggctatcctt tctgctagac tttctaagtc tcgtagactt
     1801 gagaacctta tcgctcagct tccaggagag aagaagaacg gacttttcgg aaaccttatc
     1861 gctctttctc ttggacttac cccaaacttc aagtctaact tcgatcttgc tgaggatgct
     1921 aagttgcagc tttctaagga tacctacgat gatgatcttg ataaccttct tgctcagatc
     1981 ggagatcagt acgctgatct tttccttgct gctaagaacc tttctgatgc tatccttctt
     2041 tctgacatcc ttagagttaa caccgagatc accaaggctc cactttctgc ttctatgatc
     2101 aagagatacg atgagcacca ccaggatctt acccttttga aggctcttgt tagacagcag
     2161 cttccagaga agtacaagga aatcttcttc gatcagtcta agaacggata cgctggatac
     2221 atcgatggag gagcttctca ggaggagttc tacaagttca tcaagccaat ccttgagaag
     2281 atggatggaa ccgaggagct tcttgttaag ttgaacagag aggatcttct tagaaagcag
     2341 agaaccttcg ataacggatc tatcccacac cagatccacc ttggagagct tcacgctatc
     2401 cttcgtagac aggaggattt ctacccattc ttgaaggata acagagagaa gatcgagaag
     2461 atccttacct tcagaatccc atactacgtt ggaccacttg ctagaggaaa ctctcgtttc
     2521 gcttggatga ccagaaagtc tgaggagacc atcacccctt ggaacttcga ggaggtaagt
     2581 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat
     2641 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt
     2701 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg
     2761 caggttgttg ataagggagc ttctgctcag tctttcatcg agagaatgac caacttcgat
     2821 aagaaccttc caaacgagaa ggttcttcca aagcactctc ttctttacga gtacttcacc
     2881 gtttacaacg agcttaccaa ggttaagtac gttaccgagg gaatgagaaa gccagctttc
     2941 ctttctggag agcagaagaa ggctatcgtt gatcttcttt tcaagaccaa cagaaaggtt
     3001 accgttaagc agttgaagga ggattacttc aagaagatcg agtgcttcga ttctgttgaa
     3061 atctctggag ttgaggatag attcaacgct tctcttggaa cctaccacga tcttttgaag
     3121 atcatcaagg ataaggattt ccttgataac gaggagaacg aggacatcct tgaggacatc
     3181 gttcttaccc ttaccctttt cgaggataga gagatgatcg aggagagact caagacctac
     3241 gctcaccttt tcgatgataa ggttatgaag cagttgaaga gaagaagata caccggatgg
     3301 ggtagacttt ctcgtaagtt gatcaacgga atcagagata agcagtctgg aaagaccatc
     3361 cttgatttct tgaagtctga tggattcgct aacagaaact tcatgcagct tatccacgat
     3421 gattctctta ccttcaagga ggacatccag aaggctcagg tttctggaca gggagattct
     3481 cttcacgagc acatcgctaa ccttgctgga tctccagcta tcaagaaggg aatccttcag
     3541 accgttaagg ttgttgatga gcttgttaag gttatgggta gacacaagcc agagaacatc
     3601 gttatcgaga tggctagaga gaaccagacc acccagaagg gacagaagaa ctctcgtgag
     3661 agaatgaaga gaatcgagga gggaatcaag gagcttggat ctcaaatctt gaaggagcac
     3721 ccagttgaga acacccagct tcagaacgag aagttgtacc tttactacct tcagaacgga
     3781 agagatatgt acgttgatca ggagcttgac atcaacagac tttctgatta cgatgttgat
     3841 cacatcgttc cacagtcttt cttgaaggat gattctatcg ataacaaggt tcttacccgt
     3901 tctgataaga acagaggaaa gtctgataac gttccatctg aggaggttgt taagaagatg
     3961 aagaactact ggagacagct tcttaacgct aagttgatca cccagagaaa gttcgataac
     4021 cttaccaagg ctgagagagg aggactttct gagcttgata aggctggatt catcaagaga
     4081 cagcttgttg agaccagaca gatcaccaag cacgttgctc agatccttga ttctcgtatg
     4141 aacaccaagt acgatgagaa cgataagttg atcagagagg ttaaggttat caccttgaag
     4201 tctaagttgg tttctgattt cagaaaggat ttccagttct acaaggttag agagatcaac
     4261 aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc tcttatcaag
     4321 aagtacccaa agttggagtc tgagttcgtt tacggagatt acaaggttta cgatgttaga
     4381 aagatgatcg ctaagtctga gcaggagatc ggaaaggcta ccgctaagta cttcttctac
     4441 tctaacatca tgaacttctt caagaccgag atcacccttg ctaacggaga gatcagaaag
     4501 agaccactta tcgagaccaa cggagagacc ggagagatcg tttgggataa gggaagagat
     4561 ttcgctaccg ttagaaaggt tctttctatg ccacaggtta acatcgttaa gaaaaccgag
     4621 gttcagaccg gaggattctc taaggagtct atccttccaa agagaaactc tgataagttg
     4681 atcgctagaa agaaggattg ggacccaaag aagtacggag gattcgattc tccaaccgtt
     4741 gcttactctg ttcttgttgt tgctaaggtt gagaagggaa agtctaagaa gttgaagtct
     4801 gttaaggagc ttcttggaat caccatcatg gagcgttctt ctttcgagaa gaacccaatc
     4861 gatttccttg aggctaaggg atacaaggag gttaagaagg atcttatcat caagttgcca
     4921 aagtactctc ttttcgagct tgagaacgga agaaagagaa tgcttgcttc tgctggagag
     4981 cttcagaagg gaaacgagct tgctcttcca tctaagtacg ttaacttcct ttaccttgct
     5041 tctcactacg agaagttgaa gggatctcca gaggataacg agcagaagca gcttttcgtt
     5101 gagcagcaca agcactacct tgatgagatc atcgagcaaa tctctgagtt ctctaagaga
     5161 gttatccttg ctgatgctaa ccttgataag gttctttctg cttacaacaa gcacagagat
     5221 aagccaatca gagagcaggc tgagaacatc atccaccttt tcacccttac caaccttggt
     5281 gctccagctg ctttcaagta cttcgatacc accatcgata gaaaaagata cacctctacc
     5341 aaggaggttc ttgatgctac ccttatccac cagtctatca ccggacttta cgagaccaga
     5401 atcgatcttt ctcagcttgg aggagataag agaccagctg ctaccaagaa ggctggacag
     5461 gctaagaaga agaagggaga cggatcaggc gggtcaacaa acctcagtga catcatagag
     5521 aaggaaactg gtaagcaact ggttattcaa gagagcattc tgatgctccc tgaggaagtc
     5581 gaggaagtta taggaaacaa gcctgagagc gatatactcg tgcatacggc gtatgacgag
     5641 agcacggatg aaaatgtgat gctcttgacc agcgatgcgc cagaatataa accctgggca
     5701 ctggttattc aggactctaa cggggaaaat aaaatcaaga tgctctcagg tggctcccca
     5761 aagaagaaac gcaaggttta gtggcggccg ccgacgtccg atcgttcaaa catttggcaa
     5821 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     5881 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     5941 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     6001 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     6061 atcccccctc gacagcttcc ggaaagggcg aattcgcaac tttgtataca aaagttgaac
     6121 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     6181 ctacataata ctgtaaaaca caacatatcc agtcactatg ccatccagct gatatcccct
     6241 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     6301 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tggaccagcc
     6361 aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga
     6421 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     6481 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     6541 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     6601 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     6661 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg
     6721 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     6781 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     6841 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     6901 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     6961 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     7021 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     7081 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     7141 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     7201 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     7261 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     7321 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     7381 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     7441 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     7501 cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     7561 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7681 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7741 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7801 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7861 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7921 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7981 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8041 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8101 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8161 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8221 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8281 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8341 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8401 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8461 acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa aaaggccatc
     8521 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     8581 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcgg
//
