LOCUS       sequence-245963-        8454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Produces equimolar expression of mNeongreen and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pDRF1-GW mNeongreen-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..8454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    93..114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(129..134,135..152,153..159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     191..207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(237..692)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(374..395)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     586..605
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    1500..1524
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1529..1538
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1535..2242
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      1790..1799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2249..2302
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2318..2327
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2324..3034
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2469..2487)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2737..2756
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3044..3068)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3130..3149)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3131..3149)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3297..3484
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3352..3376)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3496..3513)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3496..3512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3505..3527)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3948..5294
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(5405..6208)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6209..6424)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6449..6467)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6535..6639
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6640..6708,6709..7500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6858..6877)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7671..8259
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8160..8179
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8413..8430
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
       61 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      121 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agcttaccgc atcaggaaat
      241 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
      301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
      361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
      421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
      481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
      541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
      601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
      661 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc caagcttcct gaaacggaga
      721 aacataaaca ggcattgctg ggatcaccca tacatcactc tgttttgcct gaccttttcc
      781 ggtaatttga aaacaaaccc ggtctcgaag cggagatccg gcgataatta ccgcagaaat
      841 aaacccatac acgagacgta gaaccagccg cacatggccg gagaaactcc tgcgagaatt
      901 tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg gcacttccag gcctcgatcg
      961 agaccgttta tccattgctt ttttgttgtc tttttccctc gttcacagaa agtctgaaga
     1021 agctatagta gaactatgag ctttttttgt ttctgttttc cttttttttt tttttacctc
     1081 tgtggaaatt gttactctca cactctttag ttcgtttgtt tgttttgttt attccaatta
     1141 tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta tgctcccctc cattagtttc
     1201 gattatataa aaaggccaaa tattgtatta ttttcaaatg tcctatcatt atcgtctaac
     1261 atctaatttc tcttaaattt tttctctttc tttcctataa caccaatagt gaaaatcttt
     1321 ttttcttcta tatctacaaa aacttttttt ttctatcaac ctcgttgata aattttttct
     1381 ttaacaatcg ttaataatta attaattgga aaataaccat tttttctctc ttttatacac
     1441 acattcaaaa gaaagaaaaa aaatataccc cagcctcgac tagtgaattc gcggccatca
     1501 caagtttgta caaaaaagca ggctgctagc caccatggtg agcaagggcg aggaggataa
     1561 catggcctct ctcccagcga cacatgagtt acacatcttt ggctccatca acggtgtgga
     1621 ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg agttaaacct
     1681 gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc atatcgggta
     1741 tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg ccgccatggt
     1801 agatggctcc ggctaccaag tccatcgcac aatgcagttt gaagatggtg cctcccttac
     1861 tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc aggtgaaggg
     1921 gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg cggactggtg
     1981 caggtcgaag aagacttacc ccaacgacaa aaccatcatc agtaccttta agtggagtta
     2041 caccactgga aatggcaagc gctaccggag cactgcgcgg accacctaca cctttgccaa
     2101 gccaatggcg gctaactatc tgaagaacca gccgatgtac gtgttccgta agacggagct
     2161 caagcactcc aagaccgagc tcaacttcaa ggagtggcaa aaggccttta ccgatgtgat
     2221 gggcatggac gagctgtaca agtccggaga gggcagagga agtcttctaa catgcggtga
     2281 cgtggaggag aatcccggcc ctggggatcc accggtcgcc accatggtga gcaagggcga
     2341 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     2401 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2461 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     2521 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     2581 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     2641 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     2701 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     2761 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     2821 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     2881 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     2941 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3001 ctccaccggc ggcatggacg agctgtacaa gtaaagcggc cgcacccagc tttcttgtac
     3061 aaagtggtgt agttaattca tgatggccgc tgcaggtcga cctcgagggg gggcccggta
     3121 cccaattcgc cctatagtga gtcgtattac gcgcggatcc agctttggac ttcttcgcca
     3181 gaggtttggt caagtctcca atcaaggttg tcggcttgtc taccttgcca aaaatttacg
     3241 aaaagatgga aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga
     3301 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     3361 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     3421 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     3481 ccggcatgcc aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3541 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3601 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     3661 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataatcg gatcgtactt
     3721 gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa acagatagta
     3781 tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat gtatgtattt
     3841 cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc gcatccccgg
     3901 ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg
     3961 tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     4021 tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa
     4081 tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     4141 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca
     4201 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa
     4261 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata
     4321 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc
     4381 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg
     4441 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat
     4501 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg
     4561 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt
     4621 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga
     4681 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga
     4741 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa
     4801 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg
     4861 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta
     4921 tactttctag ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
     4981 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
     5041 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
     5101 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
     5161 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
     5221 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
     5281 tttgatattg gatcgatccg atgataagct gtcaaacatg agaattgggt aataactgat
     5341 ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag
     5401 ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac
     5461 gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa
     5521 tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc
     5581 ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc
     5641 cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt
     5701 caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta
     5761 acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa
     5821 caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt
     5881 atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt
     5941 cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca
     6001 tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg
     6061 cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg
     6121 tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag
     6181 cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcat gtttttgttc
     6241 tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta
     6301 tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg
     6361 aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat
     6421 tgaaaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6481 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6601 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6661 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6721 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6781 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6841 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6901 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6961 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7021 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7081 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7141 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7201 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7261 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7321 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7381 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7441 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7501 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7561 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7621 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7681 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7741 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7801 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7861 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7921 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7981 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8041 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8101 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8161 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8221 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8281 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8341 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8401 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgc
//
