LOCUS       sequence-245966-       11586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9-10xFlag-TdT in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, 
            Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC
  TITLE     Lineage tracing and analog recording in mammalian cells by 
            single-site DNA writing.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 
            10.1038/s41589-021-00769-8. Epub 2021 Mar 22.
  PUBMED    33753928
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00769-8"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "739-747"
COMMENT     Sequence Label: pTBL472
FEATURES             Location/Qualifiers
     source          1..11586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      911..920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             935..5038
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             935..5038
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             935..5038
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5051..5071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5078..5209
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     CDS             5261..5392
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     primer_bind     complement(7021..7038)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7027..7251
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7297..7725
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7384..7403)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7594..7615
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7734..7754)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7739..8068
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7919..8054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7981..8000
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9259..9380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9296..9315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9350..9369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9429..9445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9429..9445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9442..9464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9453..9469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9477..9483,9484..9501,9502..9507))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9522..9543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9660..9677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9831..10419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9911..9930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10590..11381,11382..11450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11213..11232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11451..11555)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cctgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctaccggtc gccaccatgg gccctagtgg cccaatggac aagaagtact ccattgggct
      961 cgatatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag
     1021 caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg
     1081 cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg
     1141 gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga
     1201 gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga
     1261 ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca
     1321 tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc
     1381 tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct
     1441 catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt
     1501 tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa
     1561 agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct
     1621 ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac
     1681 ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga
     1741 cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct
     1801 ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa
     1861 cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca
     1921 ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga
     1981 aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca
     2041 ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct
     2101 gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag
     2161 catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt
     2221 ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc
     2281 ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc
     2341 agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca
     2401 gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc
     2461 taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata
     2521 cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt
     2581 ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt
     2641 caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc
     2701 atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa
     2761 tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag
     2821 ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa
     2881 acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg
     2941 gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc
     3001 caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca
     3061 gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg
     3121 tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa
     3181 agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac
     3241 tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa
     3301 agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga
     3361 gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga
     3421 catcaatcgg ctctccgact acgacgtgga tcatatcgtg ccccagtctt ttctcaaaga
     3481 tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa
     3541 cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc
     3601 caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc
     3661 tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa
     3721 gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact
     3781 gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga
     3841 ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct
     3901 gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt
     3961 ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat
     4021 aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga
     4081 gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac
     4141 aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat
     4201 gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag
     4261 tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa
     4321 gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt
     4381 ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat
     4441 ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga
     4501 ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg
     4561 ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc
     4621 ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc
     4681 cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat
     4741 catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa
     4801 ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat
     4861 tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac
     4921 caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca
     4981 tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag
     5041 cagggctgac cccaagaaga agaggaaggt gggtaccgac tacaaggacg acgatgacaa
     5101 ggattacaaa gatgacgacg ataagcttat ggactacaag gacgacgatg acaaggatta
     5161 caaagatgac gacgataagc ttatggacta caaggacgac gatgacaagc atatgggcgc
     5221 gccacttcta aataagcgaa tttcttatga tttaggtacc gactacaagg acgacgatga
     5281 caaggattac aaagatgacg acgataagct tatggactac aaggacgacg atgacaagga
     5341 ttacaaagat gacgacgata agcttatgga ctacaaggac gacgatgaca agcatatggg
     5401 cgcgccactt ctaaataagc gaatttctta tgatttaggc cggccaatgg atccaccacg
     5461 agcgtcccac ttgagccctc ggaagaagag accccggcag acgggtgcct tgatggcctc
     5521 ctctcctcaa gacatcaaat ttcaagattt ggtcgtcttc attttggaga agaaaatggg
     5581 aaccacccgc agagcgttcc tcatggagct ggcccgcagg aaagggttca gggttgaaaa
     5641 tgagctcagt gattctgtca cccacattgt agcagagaac aactcgggtt cggatgttct
     5701 ggagtggctt caagcacaga aagtacaagt cagctcacaa ccagagctcc tcgatgtctc
     5761 ctggctgatc gaatgcatag gagcagggaa accggtggaa atgacaggaa aacaccagct
     5821 tgttgtgaga agagactatt cagatagcac caacccaggc cccccgaaga ctccaccaat
     5881 tgctgtacaa aagatctccc agtatgcgtg tcagagaaga accactttaa acaactgtaa
     5941 ccagatattc acggatgcct ttgatatact ggctgaaaac tgtgagttta gagaaaatga
     6001 agactcctgt gtgacattta tgagagcagc ttctgtattg aaatctctgc cattcacaat
     6061 catcagtatg aaggacacag aaggaattcc ctgcctgggg tccaaggtga agggtatcat
     6121 agaggagatt attgaagatg gagaaagttc tgaagttaaa gctgtgttaa atgatgaacg
     6181 atatcaatcc ttcaaactct ttacttctgt atttggagtg gggctgaaga cttctgagaa
     6241 gtggttcagg atgggtttca gaactctgag taaagtaagg tcggacaaaa gcctgaaatt
     6301 tacacgaatg cagaaagcag gatttctgta ttatgaagac cttgtcagct gtgtgaccag
     6361 ggcagaagca gaggccgtca gtgtgctggt taaagaggct gtctgggcat ttcttccgga
     6421 tgctttcgtc accatgacag gagggttccg gaggggtaag aagatggggc atgatgtaga
     6481 ttttttaatt accagcccag gatcaacaga ggatgaagag caacttttac agaaagtgat
     6541 gaacttatgg gaaaagaagg gattactttt atattatgac cttgtggagt caacatttga
     6601 aaagctcagg ttgcctagca ggaaggttga tgctttggat cattttcaaa agtgctttct
     6661 gattttcaaa ttgcctcgtc aaagagtgga cagtgaccag tccagctggc aggaaggaaa
     6721 gacctggaag gccatccgtg tggatttagt tctgtgcccc tacgagcgtc gtgcctttgc
     6781 cctgttggga tggactggct cccggcagtt tgagagagac ctccggcgct atgccacaca
     6841 tgagcggaag atgattctgg ataaccatgc tttatatgac aagaccaaga ggatattcct
     6901 caaagcagaa agtgaagaag aaatttttgc gcatctggga ttggattata ttgaaccgtg
     6961 ggaaagaaat gcctaggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag
     7021 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     7081 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     7141 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     7201 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     7261 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     7321 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7381 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7441 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7501 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7561 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7741 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7801 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7861 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7921 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7981 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     8041 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     8101 atctgatcaa gagacaggat gaggatcgtt tcgcatgcag ctctcggagg gcgaagaatc
     8161 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     8221 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     8281 tccggaagtg cttgacattg gggaatttag cgagagcctg acctattgca tctcccgccg
     8341 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcaacc
     8401 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     8461 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     8521 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     8581 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     8641 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     8701 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     8761 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     8821 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     8881 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     8941 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     9001 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     9061 cactcgtccg agggcaaagg aataggcggg actctggggt tcgaaatgac cgaccaagcg
     9121 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     9181 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     9241 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9361 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     9421 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9481 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9541 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9601 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9661 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9721 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9781 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9841 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9901 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9961 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10021 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10081 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10141 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10201 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10261 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10321 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10381 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10441 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10501 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10561 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10621 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10681 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10741 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10801 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10861 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10921 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10981 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11041 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11101 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11161 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11221 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11281 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11341 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11401 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11461 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11521 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11581 tacgtc
//
