LOCUS       sequence-245969-       11312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of mouse Sik3 cDNA with neomycin 
            resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Tarumoto Y, Lin S, Wang J, Milazzo JP, Xu Y, Lu B, Yang Z, Wei Y, 
            Polyanskaya S, Wunderlich M, Gray NS, Stegmaier K, Vakoc CR
  TITLE     Salt-Inducible Kinase inhibition suppresses acute myeloid leukemia 
            progression in vivo.
  JOURNAL   Blood. 2019 Nov 1. pii: 422697. doi: 10.1182/blood.2019001576.
  PUBMED    31697837
REFERENCE   2  (bases 1 to 11312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2019
            Nov 1. pii: 422697. doi: 10.1182/blood.2019001576."
COMMENT     Sequence Label: LentiV_Neo_Sik3
FEATURES             Location/Qualifiers
     source          1..11312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2119
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6284..6340
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6359..7153
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6413..6432)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7023..7042
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7169..7757
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7222..7242)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7640..7651)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7829..8062
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8140..8261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8177..8196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8231..8250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8301..8436
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8456..8475)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8457..8475)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8485..8502)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8485..8501)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8494..8516)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8643..9098
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8730..8749)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8940..8961
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9124..9228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9229..9297,9298..10089)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9447..9466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10260..10848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10749..10768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11002..11019
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11136..11157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11172..11177,11178..11195,11196..11202)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11210..11226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11215..11237
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11234..11250
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11234..11250
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11269..11289
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11271..11289
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc cttttcccga
     2041 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     2101 gtttgccgcc agaacacagg tgtcgtgacg cgggatccgc cacccggagg tttcactgca
     2161 caacaagatg gcggcggcgg cggcgagcgg agctggcggg gttgccgtgg ccggagcagg
     2221 gggagccggg ccggccggcc gcctgctgcc tccgcccgcc gcggggcccc cggcagcccc
     2281 cgccgcagtg cccccggccg ccgtcccggc gcgtcccaca gccccggcct cccgcgggtc
     2341 gatggccgct cgcatcggct actacgagat cgaccgcacc atcggcaagg gcaacttcgc
     2401 tgtggtcaag cgggccacgc acctcgtcac caaggccaag gttgctatta aaatcataga
     2461 taagagccag ctggatgaag aaaacttgaa gaagattttc cgggaggttc agataatgaa
     2521 gatgctttgc catccacaca tcatcagact ctaccaggtc atggagacag agcgcatgat
     2581 ttacctggtg acagaatacg ctagcggagg ggagatattt gaccacttgg tagcccatgg
     2641 aagaatggca gagaaggaag ctcgacggaa gttcaaacag atcgtcacag cggtgtattt
     2701 ttgtcactgt cggaatatcg ttcatcgtga tttaaaagcc gaaaacttac ttctggacgc
     2761 caatctgaat atcaaaatag cagactttgg cttcagcaac ctcttcactc cagggcagct
     2821 gctgaagacg tggtgtggca gccctcccta tgccgcccca gagctcttcg aagggaagga
     2881 atatgatggg cccaaagtgg acatatggag ccttggagtt gtcctctatg tgctggtgtg
     2941 tggcgccctg ccgtttgatg ggagcacact gcagaatctg cgggcccgcg tgctgagtgg
     3001 caagttccgc atcccgttct ttatgtccac agagtgtgag cacttgatcc gccacatgct
     3061 ggtgttagat ccaaataagc gcctctcaat ggaacagatc tgcaggcaca agtggatgaa
     3121 gctcggagat gcagacccca actttgacag gttaatagcg gagtgccagc aactgaagga
     3181 ggaaaggcag tcggaccccc tcaacgatga tgtcctcttg gctatggaag acatggggct
     3241 ggacaaggag cgcacacttc agtcattaag gtcagatgcc tatgaccact atagtgcaat
     3301 ctacagcctg ctgtgcgatc gacacaagaa acataaaact ctgcgtcccg gagcacttcc
     3361 cagcatgccc caagccatga ctttccaggc accagtcaat ctccaggcgg agcagacagg
     3421 cactgctatg aaccttagcg tccctcaagt tcagctgatc aacccagaga accaaattat
     3481 agagcctgac ggggctgtga acttggacag tgatgagggt gaagagcctt ctccagaagc
     3541 cttggttcgc tatttgtcaa tgaggaggca cacggtggga gtggctgacc cacgcacgga
     3601 agttatggaa gatctgcaga agctgctgcc tggctttcct ggagtaaacc cacagggtcc
     3661 attcctacaa gtggccccta acatgaactt cacgcacaac ctgttgccca tgcagagtct
     3721 gcagccaact gggcagcttg agtacaagga acagtctctg ttacagccgc ctacactaca
     3781 gctactgaac ggaatggggc cccttggccg gagagcctca gatggaggcg ccaacatcca
     3841 actgcatgcc cagcagctgc tcaagcgccc acggggaccg tccccacttg tcaccatgac
     3901 accagcagtg ccagcagtta cccctgtgga tgaggagagc tcggatgggg agccagatca
     3961 ggaagctgtg cagaggtact tggcaaatag gtccaaaagg cacacactgg ccatgaccag
     4021 cccgacagct gagatcccac cggacctgca acggcagcta ggacaacagt ctttccgttc
     4081 ccgggtctgg cctcctcacc tggtacctga ccagcatcgc tccacctaca aggactccaa
     4141 caccctgcat ctccctacgg agcgtttctc ccccgtgcgc cggttctcag acggggctgc
     4201 aagcatccag gccttcaaag ctcacctgga aaaaatgggc aacagcagca gcatcaagca
     4261 gttgcagcag gagtgtgagc agctgcagaa gatgtacggg gggcaggtgg atgagaggac
     4321 cctggagaag acccagcagc agcatatgtt gtaccagcag gagcagcacc atcagatcct
     4381 ccagcagcaa atccaagatt ctatttgtcc tcctcagccc tccccacctc ttcaggttgc
     4441 ctgtgaaaac caaccagccc tcctcaccca ccagctccag aggttaagga ttcaaccttc
     4501 aagcccaccc cccaaccatc ccagcaacca tctcttcaga cagccaagta acagtcctcc
     4561 cccggtcagc agtgccatga tcacgtctca cggtgctacg tctccctccc agtttcaagg
     4621 cttaccctcc catggtgcaa tcttccagca gcaacccgag aactgttccc cgcctcccag
     4681 tgtagcacta acctgcctgg gcctacagca ggctagccag tcacagccag tgactatcca
     4741 gctacaggag ccagttgaca tgctcagcaa catggccgga accgcggcag gctctgcagg
     4801 gcggagcatc cccatcagcc ccagcgccag tcagattcag atacagcacc gagccagcct
     4861 aatggctccc ttcagctatg ggcaccggcc cttgtccaag cagctgagcg ctgacagtgc
     4921 agaggcccac agcttgaaca tgaatcggtt ctcccctgcc aactacgacc aggcgcattt
     4981 acacccccat ctgttttcgg accagtcccg aggttccccc agcagctaca gcccttcaac
     5041 aggagtgggg tttcctccaa ctcaagccct gaaagttcct ccgcttgacc agttccccac
     5101 tttccctccc agtgcccagc agcagccacc ccactatacc acgtcagcac tacagcaggc
     5161 cctgctatct cctacaccgc cagactatcc ccgacaccag caggttcccc atatccttca
     5221 aggactgctc tctccccggc attcactcac cggccactcg gacattcggc tgcctccggc
     5281 agagtttgca cagctcatca aaaggcagca acagcatcga cagcagcagc agcagcagca
     5341 gcagcaacaa gaataccatg aattgttcag gcacatgaac caaggggatg ctgttagcct
     5401 agctcccagc ctcgggggac agaatatgac agagcaacag gctttatctt atcaaaatgc
     5461 tgactcgtac caccgccacc acaccagccc ccagcatatc ttacagatca gggcgcaaga
     5521 ttgtatctca cagggtccct cgcccacccc cacccatggc tatgcccatc agccaccact
     5581 aatgcattcg gagagtatgg aggaagactg cttgtgtgag gggctcaagg agggcttccc
     5641 agacaagagc tcaagcacac tgaccaaagg ttgccacaac agccctctgc tcttgtgtac
     5701 cagtgggcct ggggaccctg agcctttgct gggaactgtg agtcaggccc gggagctggg
     5761 gatccatccc tacggacacc agccaactgc caccacgttc agtagaaata aggtgcccag
     5821 ccgagagtct gtcctaggga actgcctgga aagaagttct cctggacaag caatggagct
     5881 gccggatcac aacggccttg ggtacccagt acggccctta gtcagtgagc acctcaggtc
     5941 ccggacgctc cagagacacc acacgatcca gaacagcgac gatgcctatg tacagctgga
     6001 taccttgcca ggaatgagcc tggtggcagg caaggcgctg agctctgccc ggatgtcaga
     6061 tgcagttctt agtcagtctt cactcatggg cagccaacag tttcaggatg aggaagatga
     6121 agaatgtggg gtgagcctgg gccacgagca tccaggcctg ggtgatggca gccagcatct
     6181 caactcctct cgctatccag ctacgtgtgt tacagacatc atgctcagcc acaagcaccc
     6241 agaggtctcc ttcagcatgg agcaagccgg cgtgggcagc ggcgccacca acttcagcct
     6301 gctgaagcag gccggcgacg tggaggagaa ccccggcccc atgaccgagt acaagcccat
     6361 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6421 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6481 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6541 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6601 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6661 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6721 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6781 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6841 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6901 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6961 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     7021 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     7081 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7141 cgagttcttc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     7201 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7261 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7321 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7381 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7441 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7501 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7561 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7621 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7681 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7741 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     7801 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7861 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7921 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7981 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8041 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     8101 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     8161 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8221 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     8281 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8341 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8401 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     8461 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8521 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8581 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8641 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8701 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8761 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8821 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8881 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8941 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     9001 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     9061 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     9121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9241 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9301 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9361 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9421 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9481 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9541 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9601 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9661 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9721 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     9781 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9841 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9901 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9961 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    10021 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    10081 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    10141 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    10321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    10381 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    10441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10861 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10921 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10981 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    11041 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    11101 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    11161 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    11221 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
    11281 cactaaaggg aacaaaagct ggagctgcaa gc
//
