LOCUS       sequence-245982-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of a guide RNA targeting an intergenic 
            region of human chromosome 2 (CRISPR cutting control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Chan EM, Shibue T, McFarland JM, Gaeta B, Ghandi M, Dumont N, 
            Gonzalez A, McPartlan JS, Li T, Zhang Y, Bin Liu J, Lazaro JB, Gu P,
            Piett CG, Apffel A, Ali SO, Deasy R, Keskula P, Ng RWS, Roberts EA, 
            Reznichenko E, Leung L, Alimova M, Schenone M, Islam M, Maruvka YE, 
            Liu Y, Roper J, Raghavan S, Giannakis M, Tseng YY, Nagel ZD, 
            D'Andrea A, Root DE, Boehm JS, Getz G, Chang S, Golub TR, Tsherniak 
            A, Vazquez F, Bass AJ
  TITLE     WRN helicase is a synthetic lethal target in microsatellite unstable
            cancers.
  JOURNAL   Nature. 2019 Apr 10. pii: 10.1038/s41586-019-1102-x. doi: 
            10.1038/s41586-019-1102-x.
  PUBMED    30971823
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Apr 10. pii: 10.1038/s41586-019-1102-x. doi: 
            10.1038/s41586-019-1102-x."
COMMENT     Sequence Label: pXPR_BRD003-sgCh2-2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    665..898
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1083..1127
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1305..1553
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1305..1325
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1476..1495
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1575..1650
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    1706..1823
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1962..3140
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2193..3131
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3088..3108
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3153..3752
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3153..3172)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3649..3669
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3768..4356
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3821..3841)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4239..4250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4428..4661
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4739..4860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4776..4795)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4830..4849
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4900..5035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5055..5074)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5056..5074)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5084..5101)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5084..5100)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5093..5115)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5242..5697
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5329..5348)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5539..5560
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5723..5827
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5828..5896,5897..6688)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6046..6065)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6859..7447
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7348..7367
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7601..7618
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7735..7756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7771..7776,7777..7794,7795..7801)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7809..7825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7814..7836
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7833..7849
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7833..7849
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7868..7888
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7870..7888
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7916..8142
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8143..8323
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
       61 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      121 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
      181 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
      241 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
      301 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
      361 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
      421 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
      481 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
      541 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
      601 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
      661 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
      721 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
      781 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
      841 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
      901 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
      961 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1021 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1081 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1141 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1201 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1261 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cagagagggc ctatttccca
     1321 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
     1381 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     1441 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     1501 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtgtg
     1561 cgtatgaagc agtggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1621 caacttgaaa aagtggcacc gagtcggtgc ttttttaagc ttggcgtaac tagatcttga
     1681 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     1741 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     1801 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     1861 gccggctcga gggggcccgg gtgcaaagat ggataaagtt ttaaacagag aggaatcttt
     1921 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     1981 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat
     2041 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     2101 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     2161 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     2221 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     2281 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     2341 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     2401 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     2461 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     2521 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     2581 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     2641 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     2701 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     2761 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     2821 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     2881 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     2941 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3001 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     3061 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     3121 ttccatttca ggtgtcgtga cgtacggcca ccatgaccga gtacaagccc acggtgcgcc
     3181 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     3241 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     3301 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     3361 gcgccgccgt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     3421 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     3481 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag
     3541 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     3601 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     3661 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     3721 cctggtgcat gacccgcaag cccggtgcct gaacgcgtta agtcgacaat caacctctgg
     3781 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3841 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3901 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3961 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4021 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     4081 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4141 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     4201 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     4261 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     4321 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     4381 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4441 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4501 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4561 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4621 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     4681 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     4741 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4801 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4861 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     4921 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4981 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     5041 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     5101 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5161 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5221 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5281 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5341 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5401 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5461 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5521 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5581 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5641 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     5701 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5761 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5821 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5881 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5941 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6001 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6061 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6121 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6181 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6241 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6301 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6361 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6421 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6481 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6541 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6601 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6661 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6721 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6781 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6841 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6901 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6961 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7021 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7081 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7141 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7201 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7261 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7321 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7381 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7441 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7501 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7561 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7621 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     7681 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7741 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7801 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     7861 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     7921 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     7981 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     8041 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     8101 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     8161 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8221 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8281 agatccctca gaccctttta gtcagtgtgg aaaatctcta gca
//
