LOCUS       sequence-245987-        4000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pMT Draper-delta ITAM-GFP
FEATURES             Location/Qualifiers
     source          1..4000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3088..3801
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             3088..3798
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(3115..3143)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3738..3758
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3823..3840)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3842..3976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3866..3885)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3920..3939
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctggctcagg cggatcttaa agatctggat ggacccaata tctgcaaaag aagagaacta
       61 tataacgtag acgttgtcta cacagaactg caatcgttcc aggaacgggg ctccacctgg
      121 tgcgtcacat tcccgccgag gtgctccacc tatcgcatta aacaccgagt ggtcaacaaa
      181 accaagacga ttgcgaagaa ccgtattgtg agggactgtt gtgatggcta catcgcgagc
      241 gcaggagaat gcgtgccgca ttgttcggaa ccctgccagc acggtagatg catctccccg
      301 gagaagtgca agtgtgatca tggttacgga ggacccgcct gcgatataaa ttgcccaccc
      361 ggctggtatg gccggaactg ctcgatgcag tgcgactgcc tgaacaacgc ggtatgtgag
      421 cccttttcag gcgactgcga gtgcgccaag ggctacactg gagcacgctg cgcggacatc
      481 tgtccggagg gctttttcgg ggccaactgc tcggagaagt gccgatgcga gaacggcggc
      541 aagtgtcacc acgtgtccgg cgaatgccag tgtgctcccg gtttcacggg tcccctatgt
      601 gatatgcgct gtccggatgg aaagcatggc gcccagtgcc agcaggactg tccctgccag
      661 aacgacggaa agtgtcagcc agagaccggg gcctgtatgt gcaatccagg ctggacgggc
      721 gacgtgtgtg caaacaagtg tccggtgggc agctacggcc ctggatgcca agagagctgc
      781 gagtgctaca agggagcgcc ctgccaccac atcaccggcc agtgcgagtg tccgcccgga
      841 tatcgcggag aacgctgctt cgacgagtgt cagctgaaca cgtacggatt caactgcagc
      901 atgacctgcg actgcgcgaa cgatgccatg tgcgatcggg cgaacggaac gtgcatctgc
      961 aaccccggct ggacgggtgc caagtgcgcc gagcggatct gcgaggcgaa caagtacggg
     1021 ctggactgta accgcacctg cgagtgcgac atggagcaca cggatctgtg tcacccggag
     1081 acgggaaatt gccagtgcag catcggatgg agcagtgctc agtgcaccag accctgcacc
     1141 ttcctgcgct acggcccgaa ttgcgagctg acctgcaact gcaagaacgg agccaagtgc
     1201 tcgccggtca atggtacgtg cctgtgtgcg ccgggctggc gcggacccac ctgcgaggag
     1261 agctgcgagc cgggcacctt tggtcaggac tgcgccctgc gctgcgactg ccagaacgga
     1321 gccaagtgcg agccggagac gggccagtgc ttgtgcacgg ccggatggaa gaacatcaag
     1381 tgcgaccggc cgtgtgatct gaaccacttc ggacaggact gcgccaaggt gtgcgactgc
     1441 cacaataacg ccgcctgcaa tccgcagaac ggaagctgca cctgtgccgc cggctggacg
     1501 ggcgagcggt gcgaaaggaa gtgcgatacc ggcaaattcg gacacgactg cgcccagaag
     1561 tgccagtgcg acttcaacaa cagcctggcc tgcgatgcca ccaatggaag atgcgtgtgc
     1621 aagcaggatt ggggaggcgt tcattgcgag accaactgtc ggagcggtta ctatggtgaa
     1681 aattgtgata aagtttgcag atgcctgaat aactcctcct gcgaccccga ttccggtaac
     1741 tgcatctgct cagccggttg gactggcgcc gactgtgcgg aaccctgccc acctggattc
     1801 tacggcatgg aatgcaagga acggtgcccg gagatcctcc atggcaacaa gagctgcgat
     1861 cacatcacag gtgaaattct ctgccgcacg ggttacatag gtctcacctg cgagcaccca
     1921 tgtcctgctg gactctacgg acccggctgc aagctcaagt gcaattgcga gcacggcggc
     1981 gagtgcaacc acgttacagg ccagtgccag tgcctgccag ggtggacagg ttccaactgc
     2041 aacgagtcct gtcccacaga cacctacgga caaggatgcg cccagcggtg ccgctgcgtg
     2101 caccacaagg tttgccgcaa ggccgacggt atgtgcatct gcgaaaccgg ctggtcgggc
     2161 acccggtgcg atgaggtctg tccggaggga ttctacggcg agcactgcat gaatacctgt
     2221 gcctgtccgt cggccaattt ccagtgccat gcggcccacg gttgtgtgtg caggagtggc
     2281 tacaccggcg ataactgcga cgagctaatc gcctctcaac gtattgcaga ccaaagcgaa
     2341 aattcgagcc gggcgagtgt agctctgacc ttagtcctga tgaccctgtt tgcgtgcata
     2401 atcttcgcag tgttcattta ctaccgccga cgtgtgtcca atctaaaaac cgaaatcgcc
     2461 catgtacact acacccatga tacgaatccc ccgagctggc cgcccaacca caacttcgac
     2521 aatcccgtgt acggcatgca ggcggaaacg cgtctcctgc ccaacaatat gcgttccaag
     2581 atgaacaact ttgaccagag gagcacgatg agcacggact acggcgatga ctgcaatgcc
     2641 agtggcaggg tgggtagcta ttcgatcaac tacaaccacg acctgctaac gaagaacttg
     2701 aacgcggacc ggaccaatcc gattgtgtac aacgaatccc tcaaagagga gcatgtcttc
     2761 gacgagatca agcacaagga gggctacaag gatcccgatg aattcgatca cctggactac
     2821 tcacgaccca gcacctcgca gaagccgcac taccaccgca tgaacgacgc catgctcaac
     2881 atcaaccagg acgaggagaa gcccagcaac gtgaagaaca tgactgtgct gctgaacaaa
     2941 ccgctgccgc ccacggaacc ggagccacaa cacgagtgct tcgacaacac gaacaccaac
     3001 ctggacaacg tgtcgacggc gtcgcctagt tccagtccga aatttctaaa gcggcgcgcc
     3061 ggcggccgct cgagtctaga gggccccagc aaaggagaag aacttttcac tggagttgtc
     3121 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt ccgtggagag
     3181 ggtgaaggtg atgctacaaa cggaaaactc acccttaaat ttatttgcac tactggaaaa
     3241 ctacctgttc cgtggccaac acttgtcact actctgacct atggtgttca atgcttttcc
     3301 cgttatccgg atcacatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
     3361 gtacaggaac gcactatatc tttcaaagat gacgggacct acaagacgcg tgctgaagtc
     3421 aagtttgaag gtgataccct tgttaatcgt atcgagttaa agggtattga ttttaaagaa
     3481 gatggaaaca ttcttggaca caaactcgag tacaacttta actcacacaa tgtatacatc
     3541 acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacgttgaa
     3601 gatggttccg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     3661 gtccttttac cagacaacca ttacctgtcg acacaatctg tcctttcgaa agatcccaac
     3721 gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc
     3781 atggatgagc tctacaaata ggtttaaacg cccgctgatc agcctcgact gtgccttcta
     3841 agatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     3901 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     3961 ctgcaataaa caagttaact ctagaggatc cggtgcgtgg
//
