LOCUS       sequence-24601-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Pluta K, Luce MJ, Bao L, Agha-Mohammadi S, Reiser J
  TITLE     Tight control of transgene expression by lentivirus vectors 
            containing second-generation tetracycline-responsive promoters.
  JOURNAL   J Gene Med. 2005 Jun . 7(6):803-17.
  PUBMED    15655804
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2005 Jun . 7(6):803-17."
COMMENT     Sequence Label: pLitTREPitt
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(164..188)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    210..228
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    247..265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    284..302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    321..339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    395..413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    432..450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    469..487
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(597..614)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(597..613)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(606..628)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aannacanna ttcgccagct acgtaatacg actcactagt gggtaccaga gctccctagg
       61 ttctagaacc ggtgacgtct cccatggtga agcttggatc caggctggat cggtcccggt
      121 gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta
      181 aacgagctct gcttatatag ggggtacctt ctctatcact gatagggagc tcgagacgtg
      241 tcgacttctc tatcactgat agggagacgt tctagacgta ccttctctat cactgatagg
      301 gagctcgaga cgtgtcgact tctctatcac tgatagggag acgttctaga cgtaccttct
      361 ctatcactga tagggagctc gagacgtgtc gacttctcta tcactgatag ggagacgttc
      421 tagacgtacc ttctctatca ctgataggga gctcgagacg tgtcgacttc tctatcactg
      481 atagggagac gtcaggtggc acttgatatc ctgcaggaat tcctcgagac cgtacgtgcg
      541 cgcgaatgca tccagatctt ccctctagtc aaggccttaa gtgagtcgta ttacggactg
      601 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      661 gcagcacatc cccctcccnc cagntggcgt aatagcgaag aggcccgctg cgatcgccct
      721 tcccaacagt tgcgcagcct gaatggcgan ggcgcttcgc ttggtaataa gcccgcttcg
      781 gcggcttttt ttgtaac
//
