LOCUS       sequence-24602-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Ricks DM, Kutner R, Zhang XY, Welsh DA, Reiser J
  TITLE     Optimized lentiviral transduction of mouse bone marrow-derived 
            mesenchymal stem cells.
  JOURNAL   Stem Cells Dev. 2008 Jun . 17(3):441-50.
  PUBMED    18513160
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Dev. 2008 Jun . 17(3):441-50."
COMMENT     Sequence Label: pNL-EGFP/EF1alpha/WPREdU3
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    52..640
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(105..125)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(523..534)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccncgggacn nncggcagga gagctgtaca agtccggact cagatctcga gaatcaacct
       61 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
      121 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
      181 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
      241 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
      301 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
      361 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
      421 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
      481 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
      541 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
      601 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgggtacct ttaagaccaa
      661 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
      721 ggctaattca ctcccaaaga agacagatct gctttttgct gtactgggtc tctctggtta
      781 gacagactga gctggaactc tctggctact aggaaccang ctaagctcat aanctgctga
      841 tgctcaga
//
