LOCUS       sequence-246020-       13657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drug inducible TALEN targeting the human AAVS1 locus for genome 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13657)
  AUTHORS   Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y
  TITLE     Multiple Chemical Inducible Tal Effectors for Genome Editing and 
            Transcription Activation.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 
            10.1021/acschembio.7b00606. Epub 2018 Jan 8.
  PUBMED    29308880
REFERENCE   2  (bases 1 to 13657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.7b00606"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "609-617"
COMMENT     Sequence Label: 2ERT2-AAVS1 TALEN-L
FEATURES             Location/Qualifiers
     source          1..13657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..341
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1205..1214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1234..1243
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1288..2223
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2284..3219
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5536..6123
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     primer_bind     complement(6334..6354)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6339..6668
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6519..6654
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6687..7481
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6741..6760)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7351..7370
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7564..8152
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7617..7637)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8035..8046)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8239..8472
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8544..8665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8684..8819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8851..8870)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8852..8870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8880..8897)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8880..8896)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8889..8911)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9041..9496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9128..9147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9338..9359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9525..9629
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9630..9698,9699..10490)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9848..9867)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10661..11249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11150..11169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11403..11420
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11537..11558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11573..11578,11579..11596,11597..11603)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11611..11627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11616..11638
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11635..11651
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11635..11651
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11670..11690
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11672..11690
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             11946..12126
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12170..12295
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12788..13021
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13206..13250
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13517..13634
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagcccggg ctcgaccata tgataagctt tgcaaagatg gataaagttt taaacagaga
       61 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      121 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      181 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      241 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      301 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      361 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      421 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      481 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      541 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      601 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      661 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      721 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      781 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      841 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
      901 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
      961 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1021 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1081 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1141 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctcctttc
     1201 tagagccacc atgggttaat tctcctttct agagccacca tgggtggcgc gcctcgcctg
     1261 ctggaagatg gcgatctcga gccatctgct ggagacatga gagctgccaa cctttggcca
     1321 agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac
     1381 cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct
     1441 accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag
     1501 ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat
     1561 gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg
     1621 cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac
     1681 cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct
     1741 cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg
     1801 cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac
     1861 catatccacc gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca
     1921 ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac
     1981 atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg
     2041 gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca tgcgcccact
     2101 agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct
     2161 acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc
     2221 acagctttaa ttattggaag tggcgcgcct cgcctgctgg aagatggcga tctcgagcca
     2281 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
     2341 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
     2401 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
     2461 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
     2521 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
     2581 gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag
     2641 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
     2701 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
     2761 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
     2821 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
     2881 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
     2941 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     3001 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     3061 gaggcggcgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     3121 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
     3181 tattacatca cgggggaggc agagggtttc cctgccacag ctttaattaa tggaagtgga
     3241 gtagatttga gaactttggg atattcacag cagcagcagg aaaagatcaa gcccaaagtg
     3301 aggtcgacag tcgcgcagca tcacgaagcg ctggtgggtc atgggtttac acatgcccac
     3361 atcgtagcct tgtcgcagca ccctgcagcc cttggcacgg tcgccgtcaa gtaccaggac
     3421 atgattgcgg cgttgccgga agccacacat gaggcgatcg tcggtgtggg gaaacagtgg
     3481 agcggagccc gagcgcttga ggccctgttg acggtcgcgg gagagctgag agggcctccc
     3541 cttcagctgg acacgggcca gttgctgaag atcgcgaagc ggggaggagt cacggcggtc
     3601 gaggcggtgc acgcgtggcg caatgcgctc acgggagcac ccctcaacct gaccccagag
     3661 caggtcgtgg caattgcgag caacaagggg ggaaagcagg cactcgaaac cgtccagagg
     3721 ttgctgcctg tgctgtgcca agcgcacgga cttacgccag agcaggtcgt ggcaattgcg
     3781 agcaacggag ggggaaagca ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc
     3841 caagcgcacg gactaacccc agagcaggtc gtggcaattg cgagccatga cgggggaaag
     3901 caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca cgggttgacc
     3961 ccagagcagg tcgtggcaat tgcgagccat gacgggggaa agcaggcact cgaaaccgtc
     4021 cagaggttgc tgcctgtgct gtgccaagcg cacggcttaa ccccagagca ggtcgtggca
     4081 attgcgagcc atgacggggg aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg
     4141 ctgtgccaag cgcacggcct gaccccagag caggtcgtgg caattgcgag ccatgacggg
     4201 ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca agcgcacgga
     4261 ctgacaccag agcaggtcgt ggcaattgcg agcaacggag ggggaaagca ggcactcgaa
     4321 accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg gactcactcc agagcaggtc
     4381 gtggcaattg cgagccatga cgggggaaag caggcactcg aaaccgtcca gaggttgctg
     4441 cctgtgctgt gccaagcgca cggcctcacc ccagagcagg tcgtggcaat tgcgagccat
     4501 gacgggggaa agcaggcact cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg
     4561 cacggattaa ccccagagca ggtcgtggca attgcgagca acatcggggg aaagcaggca
     4621 ctcgaaaccg tccagaggtt gctgcctgtg ctgtgccaag cgcacggact taccccagag
     4681 caggtcgtgg caattgcgag ccatgacggg ggaaagcagg cactcgaaac cgtccagagg
     4741 ttgctgcctg tgctgtgcca agcgcacggg ctcaccccag agcaggtcgt ggcaattgcg
     4801 agccatgacg ggggaaagca ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc
     4861 caagcgcacg gtcttacccc agagcaggtc gtggcaattg cgagccatga cgggggaaag
     4921 caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca cggcctcacc
     4981 ccagagcagg tcgtggcaat tgcgagccat gacgggggaa agcaggcact cgaaaccgtc
     5041 cagaggttgc tgcctgtgct gtgccaagcg catgggctca ccccagagca ggtcgtggca
     5101 attgcgagca acatcggggg aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg
     5161 ctgtgccaag cgcacggtct aaccccagag caggtcgtgg caattgcgag ccatgacggg
     5221 ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca agcgcacgga
     5281 ctcacgcctg agcaggtagt ggctattgca tccaacatcg ggggcagacc cgcactggag
     5341 tcaatcgtgg cccagctttc gaggccggac cccgcgctgg ccgcactcac taatgatcat
     5401 cttgtagcgc tggcctgcct cggcggacga cccgccttgg atgcggtgaa gaaggggctc
     5461 ccgcacgcgc ctgcattgat taagcggacc aacagaagga ttcccgagag gacatcacat
     5521 cgagtggcag gttcccaact cgtgaagagt gaacttgagg agaaaaagtc ggagctgcgg
     5581 cacaaattga aatacgtacc gcatgaatac atcgaactta tcgaaattgc taggaactcg
     5641 actcaagaca gaatccttga gatgaaggta atggagttct ttatgaaggt ttatggatac
     5701 cgagggaagc atctcggtgg atcacgaaaa cccgacggag caatctatac ggtggggagc
     5761 ccgattgatt acggagtgat cgtcgacacg aaagcctaca gcggtgggta caatcttccc
     5821 atcgggcagg cagatgagat gcaacgttat gtcgaagaaa atcagaccag gaacaaacac
     5881 atcaatccaa atgagtggtg gaaagtgtat ccttcatcag tgaccgagtt taagtttttg
     5941 tttgtctctg ggcatttcaa aggcaactat aaggcccagc tcacacggtt gaatcacatt
     6001 acgaactgca atggtgcggt tttgtccgta gaggaactgc tcattggtgg agaaatgatc
     6061 aaagcgggaa ctctgacact ggaagaagtc agacgcaagt ttaacaatgg cgagatcaat
     6121 ttccgctcat aacctgcagg ggaagtggaa gtgatatcgg aagtggaagc actagtccgg
     6181 aagtttcgaa gtcgacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6241 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6301 catcaatgta tcttatacta gtgtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa
     6361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6601 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6661 tttgcaaaaa gcttggatcc gccaccatga ttgaacaaga tggattgcac gcaggttctc
     6721 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6781 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6841 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6901 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6961 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     7021 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     7081 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     7141 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     7201 ccaggctcaa ggcgcgcatg cccgacggcg atgatctcgt cgtgacccat ggcgatgcct
     7261 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7321 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7381 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7441 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aatcgataaa ataaaagatt
     7501 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagctc
     7561 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7621 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7681 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7741 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7801 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7861 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7921 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7981 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8041 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8101 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     8161 cgagctcagt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8221 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     8281 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     8341 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     8401 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     8461 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     8521 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     8581 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8641 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     8701 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8761 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8821 aggcttttgc gtcgagacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     8881 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8941 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     9001 ccttcccaac agttgcgcag cctgaatggc gaatggcgcg acgcgccctg tagcggcgca
     9061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     9421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     9481 ttaacgttta caatttccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9601 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9661 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9721 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     9781 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     9841 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     9901 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     9961 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
    10021 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
    10081 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
    10141 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
    10201 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
    10261 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    10321 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    10381 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    10441 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    10501 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    10561 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    10621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    10681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    10741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    10801 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    10861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    10921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    10981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    11041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    11101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    11161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    11221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    11281 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    11341 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    11401 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
    11461 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    11521 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    11581 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    11641 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    11701 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
    11761 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
    11821 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
    11881 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa
    11941 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    12001 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    12061 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    12121 ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg
    12181 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
    12241 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
    12301 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
    12361 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
    12421 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
    12481 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
    12541 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
    12601 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
    12661 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
    12721 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
    12781 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
    12841 cctcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
    12901 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    12961 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    13021 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    13081 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
    13141 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    13201 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
    13261 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    13321 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    13381 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
    13441 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
    13501 gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
    13561 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
    13621 aaattcaaaa ttttatcgat cacgagacta gcctcga
//
