LOCUS       sequence-246025-        4913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast tagging vector to create a gene fusion with yeast optimized 
            mNeongreen with CaUra3 selection. *An updated version of this 
            plasmid is available: #168059 pFA6a-link-ymNeongreen-URA3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-ymNeongreen-CaURA3
FEATURES             Location/Qualifiers
     source          1..4913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     18..37
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        118..136
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     118..135
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      460..469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      956..1143
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1011..1035)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        1218..1561
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      2380..2577
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2684..2703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2685..2703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2790..2807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2961..3549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3041..3060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3720..4511,4512..4580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4343..4362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4581..4685)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4753..4771
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4809..4831)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat
       61 attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
      121 taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag gtcgacggat
      181 cggtgacggt gctggtttaa ttaaatggtc tctaagggtg aagaagataa catggcttct
      241 ttgccagcca cccacgagtt gcacatcttc ggttccatta acggtgtcga cttcgatatg
      301 gtcggtcaag gtaccggtaa cccaaacgac ggttacgaag aattgaactt gaagtctacc
      361 aagggcgact tgcagttctc cccttggatt ttggttccac acatcggtta cggtttccac
      421 caatacttgc catacccaga cggtatgtcc ccattccaag ccgccatggt tgacggttct
      481 ggttaccaag tccacagaac catgcaattc gaagacggtg cttccttgac cgtgaactac
      541 agatacacct acgaaggttc tcacatcaag ggcgaagccc aagttaaggg taccggtttc
      601 cctgctgacg gtccagtcat gactaactcc ttgaccgctg ctgactggtg tcgttccaag
      661 aagacctacc caaacgataa gaccatcatt tccactttca agtggtccta caccaccggt
      721 aacggcaaga gataccgctc taccgctcgt accacctaca ccttcgccaa gccaatggct
      781 gccaactact tgaagaacca gcctatgtac gtcttccgta agaccgagct gaagcattcc
      841 aagactgaat tgaacttcaa ggaatggcaa aaggctttca ccgacgtcat gggtatggac
      901 gaattgtaca agtaagcggc cgcctcgagg tcgacggcgc gccacttcta aataagcgaa
      961 tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa
     1021 attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta actctttcct
     1081 gtaggtcagg ttgctttctc aggtatagta tgaggtcgct cttattgacc acacctctac
     1141 cggcagatcc gctagggata acagggtaat atagatctgt ttagcttgcc tcgtccccgc
     1201 cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc
     1261 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
     1321 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
     1381 cgctgcggac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
     1441 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
     1501 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
     1561 catgacagtc aacactaaga cctatagtga gagagcagaa actcatgcct caccagtagc
     1621 acaacgatta tttcgattaa tggaactgaa gaaaaccaat ttatgtgcat caattgatgt
     1681 tgataccact aaggaattcc ttgaattaat tgataaattg ggtccttatg tatgcttaat
     1741 caagactcat attgatataa tcaatgattt ttcctatgaa tccactattg aaccattatt
     1801 agaactttca cgtaaacatc aatttatgat ttttgaagat agaaaatttg ctgatattgg
     1861 taataccgtg aagaaacaat atattggtgg agtttataaa attagtagtt gggcagatat
     1921 tactaatgct catggtgtca ctgggaatgg agtagttgaa ggattaaaac agggagctaa
     1981 agaaaccacc accaaccaag agccaagagg gttattgatg ttagctgaat tatcatcagt
     2041 gggatcatta gcatatggag aatattctca aaaaactgtt gaaattgcta aatccgataa
     2101 ggaatttgtt attggattta ttgcccaacg tgatatgggt ggacaagaag aaggatttga
     2161 ttggcttatt atgacacctg gagttggatt agatgataaa ggtgatggat taggacaaca
     2221 atatagaact gttgatgaag ttgttagcac tggaactgat attatcattg ttggtagagg
     2281 attgtttggt aaaggaagag atccagatat tgaaggtaaa aggtatagag atgctggttg
     2341 gaatgcttat ttgaaaaaga ctggccaatt ataatcagta ctgacaataa aaagattctt
     2401 gttttcaaga acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca
     2461 aatgttagcg tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta
     2521 agtgcgcaga aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg
     2581 tcgattcgat actaacgccg ccatccagtg tcgaaaacga gctcgaattc atcgatgata
     2641 tcagatccac tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta
     2701 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
     2761 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3361 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3601 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3661 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3721 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3781 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3841 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3901 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3961 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     4021 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4081 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4141 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4201 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4261 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4321 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4381 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4441 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4501 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4561 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4621 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4681 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4741 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     4801 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     4861 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcg
//
