LOCUS       sequence-246028-       11829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human 
            NGN2 ene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11829)
  AUTHORS   Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y
  TITLE     Multiple Chemical Inducible Tal Effectors for Genome Editing and 
            Transcription Activation.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 
            10.1021/acschembio.7b00606. Epub 2018 Jan 8.
  PUBMED    29308880
REFERENCE   2  (bases 1 to 11829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.7b00606"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "609-617"
COMMENT     Sequence Label: EF1a-3NLS-NGN2TALE-GCN4
FEATURES             Location/Qualifiers
     source          1..11829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        133..344
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1208..1217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1229..1249
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1253..1273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1277..1297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4004..4060
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4076..4132
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4148..4204
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4220..4276
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4292..4348
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4364..4420
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4436..4492
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4508..4564
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4580..4636
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4652..4708
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(4932..4952)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4937..5266
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5117..5252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5276..5650
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    5736..6324
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5789..5809)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6207..6218)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6411..6644
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6716..6837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6856..6991
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7023..7042)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7024..7042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7052..7069)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7052..7068)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7061..7083)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7213..7668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7300..7319)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7510..7531
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7697..7801
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7802..7870,7871..8662)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8020..8039)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8833..9421
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9322..9341
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9575..9592
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9709..9730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9745..9750,9751..9768,9769..9775)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9783..9799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9788..9810
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9807..9823
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9807..9823
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9842..9862
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9844..9862
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             10118..10298
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10342..10467
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10960..11193
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11378..11422
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11689..11806
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagcccggg ctcgatcgac atatgataag ctttgcaaag atggataaag ttttaaacag
       61 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
      121 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      181 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      241 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      301 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      361 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      421 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
      481 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
      541 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
      601 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
      661 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
      721 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
      781 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
      841 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
      901 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
      961 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     1021 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     1081 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     1141 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     1201 ttctagagcc accatgggtg gcgcgcctcc aaaaaagaag agaaaggtag atccaaaaaa
     1261 gaagagaaag gtagatccaa aaaagaagag aaaggtagga ttaattaatg gaagtggaag
     1321 tggaagtgga ggtaccccgg atgttccaga ttacgcttcg cggacccggc tcccttcccc
     1381 acccgcaccc agcccagcgt tttcggccga ctcgttctca gacctgctta ggcagttcga
     1441 cccctcactg tttaacacat cgttgttcga ctcccttcct ccgtttgggg cgcaccatac
     1501 ggaggcggcc accggggagt gggatgaggt gcagtcggga ttgagagctg cggatgcacc
     1561 acccccaacc atgcgggtgg ccgtcaccgc tgcccgaccg ccgagggcga agcccgcacc
     1621 aaggcggagg gcagcgcaac cgtccgacgc aagccccgca gcgcaagtag atttgagaac
     1681 tttgggatat tcacagcagc agcaggaaaa gatcaagccc aaagtgaggt cgacagtcgc
     1741 gcagcatcac gaagcgctgg tgggtcatgg gtttacacat gcccacatcg tagccttgtc
     1801 gcagcaccct gcagcccttg gcacggtcgc cgtcaagtac caggacatga ttgcggcgtt
     1861 gccggaagcc acacatgagg cgatcgtcgg tgtggggaaa cagtggagcg gagcccgagc
     1921 gcttgaggcc ctgttgacgg tcgcgggaga gctgagaggg cctccccttc agctggacac
     1981 gggccagttg ctgaagatcg cgaagcgggg aggagtcacg gcggtcgagg cggtgcacgc
     2041 gtggcgcaat gcgctcacgg gagcacccct caacctgacc ccagagcagg tcgtggcaat
     2101 tgcgagccat aacgggggaa agcaggcact cgaaaccgtc cagaggttgc tgcctgtgct
     2161 gtgccaagcg cacggactta cgccagagca ggtcgtggca attgcgagca acatcggggg
     2221 aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg ctgtgccaag cgcacggact
     2281 aaccccagag caggtcgtgg caattgcgag caacatcggg ggaaagcagg cactcgaaac
     2341 cgtccagagg ttgctgcctg tgctgtgcca agcgcacggg ttgaccccag agcaggtcgt
     2401 ggcaattgcg agcaacggag ggggaaagca ggcactcgaa accgtccaga ggttgctgcc
     2461 tgtgctgtgc caagcgcacg gcttaacccc agagcaggtc gtggcaattg cgagccataa
     2521 cgggggaaag caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca
     2581 cggcctgacc ccagagcagg tcgtggcaat tgcgagcaac atcgggggaa agcaggcact
     2641 cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg cacggactga caccagagca
     2701 ggtcgtggca attgcgagca acggaggggg aaagcaggca ctcgaaaccg tccagaggtt
     2761 gctgcctgtg ctgtgccaag cgcacggact cactccagag caggtcgtgg caattgcgag
     2821 ccataacggg ggaaagcagg cactcgaaac cgtccagagg ttgctgcctg tgctgtgcca
     2881 agcgcacggc ctcaccccag agcaggtcgt ggcaattgcg agcaacatcg ggggaaagca
     2941 ggcactcgaa accgtccaga ggttgctgcc tgtgctgtgc caagcgcacg gattaacccc
     3001 agagcaggtc gtggcaattg cgagcaacgg agggggaaag caggcactcg aaaccgtcca
     3061 gaggttgctg cctgtgctgt gccaagcgca cggacttacc ccagagcagg tcgtggcaat
     3121 tgcgagcaac atcgggggaa agcaggcact cgaaaccgtc cagaggttgc tgcctgtgct
     3181 gtgccaagcg cacgggctca ccccagagca ggtcgtggca attgcgagca acatcggggg
     3241 aaagcaggca ctcgaaaccg tccagaggtt gctgcctgtg ctgtgccaag cgcacggtct
     3301 taccccagag caggtcgtgg caattgcgag caacggaggg ggaaagcagg cactcgaaac
     3361 cgtccagagg ttgctgcctg tgctgtgcca agcgcacggc ctcaccccag agcaggtcgt
     3421 ggcaattgcg agcaacatcg ggggaaagca ggcactcgaa accgtccaga ggttgctgcc
     3481 tgtgctgtgc caagcgcatg ggctcacccc agagcaggtc gtggcaattg cgagccatga
     3541 cgggggaaag caggcactcg aaaccgtcca gaggttgctg cctgtgctgt gccaagcgca
     3601 cggtctaacc ccagagcagg tcgtggcaat tgcgagccat aacgggggaa agcaggcact
     3661 cgaaaccgtc cagaggttgc tgcctgtgct gtgccaagcg cacggactca cgcctgagca
     3721 ggtagtggct attgcatcca ataacggggg cagacccgca ctggagtcaa tcgtggccca
     3781 gctttcgagg ccggaccccg cgctggccgc actcactaat gatcatcttg tagcgctggc
     3841 ctgcctcggc ggacgacccg ccttggatgc ggtgaagaag gggctcccgc acgcgcctgc
     3901 attgattaag cggaccaaca gaaggattcc cgagaggaca tcacatcgag tggcagctag
     3961 cggcggatcc gccatcatac acgcggccgc aacaagcggt ccggaagaac ttttgagcaa
     4021 gaattatcat cttgagaacg aagtggctcg tcttaagaaa ggttctggca gtggagaaga
     4081 actgctttca aagaattacc acctggaaaa tgaggtagct agactgaaaa aggggagcgg
     4141 aagtggggag gagttgctga gcaaaaatta tcatttggag aacgaagtag cacgactaaa
     4201 gaaagggtcc ggatcgggtg aggagttact ctcgaaaaat tatcatctcg aaaacgaagt
     4261 ggctcggcta aaaaagggca gtggttctgg agaagagcta ttatctaaaa actaccacct
     4321 cgaaaatgag gtggcacgct taaaaaaggg aagtggcagt ggtgaagagc tactatccaa
     4381 gaattatcat cttgagaacg aggtagcgcg tttgaagaag ggttccggct caggagagga
     4441 actgctctcg aagaactatc atcttgaaaa tgaggtcgct cgattaaaaa agggatcggg
     4501 cagtggtgag gaactacttt caaagaatta ccacctcgaa aacgaagtag ctcgattaaa
     4561 gaaaggttca gggtcgggtg aagaattact gagtaaaaat tatcatctgg aaaatgaggt
     4621 agcgagacta aaaaagggga gtggttctgg cgaggaattg ctatcgaaaa attatcatct
     4681 tgagaacgaa gttgctaggc tcaaaaaggg ctcaggctca ggctgagttt aaacatcgcc
     4741 tgcaggggaa gtggaagtga tatcggaagt ggaagcacta gtggaagttt cgaagtcgac
     4801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     4921 gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5041 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     5101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     5161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     5221 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccatggc
     5281 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     5341 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     5401 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     5461 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     5521 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     5581 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     5641 gcaggactga ccgatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     5701 tgaaagaccc cacctgtagg tttggcaagc tcgacaatca acctctggat tacaaaattt
     5761 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5821 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5881 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5941 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6001 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6061 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6121 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6181 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6241 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6301 tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gaacctttaa gaccaatgac
     6361 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6421 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6481 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6541 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6601 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6661 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6721 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6781 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6841 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6901 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     6961 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcgtcgagac gtacccaatt
     7021 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     7081 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7141 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7201 gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7261 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7321 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7381 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7441 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7501 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7561 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7621 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc caggtggcac
     7681 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7741 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7801 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7861 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7921 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7981 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8041 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8101 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8161 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8221 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8281 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8341 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8401 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8461 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8521 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8581 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8641 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8701 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8761 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8821 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8881 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8941 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     9001 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9061 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9121 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9181 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9241 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9301 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9361 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9421 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9481 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9541 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9601 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9661 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9721 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9781 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9841 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     9901 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     9961 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
    10021 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
    10081 gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt tagaccagat
    10141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    10201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    10261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacctg
    10321 aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    10381 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    10441 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    10501 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    10561 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    10621 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    10681 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    10741 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    10801 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    10861 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    10921 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    10981 cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg tacaggccag
    11041 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    11101 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    11161 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    11221 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    11281 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    11341 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    11401 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    11461 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    11521 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    11581 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    11641 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
    11701 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    11761 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac
    11821 tagcctcga
//
