LOCUS       sequence-246030-       11763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human 
            SOX2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y
  TITLE     Multiple Chemical Inducible Tal Effectors for Genome Editing and 
            Transcription Activation.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 
            10.1021/acschembio.7b00606. Epub 2018 Jan 8.
  PUBMED    29308880
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.7b00606"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "609-617"
COMMENT     Sequence Label: EF1a-3NLS-Sox2TALE-GCN4
FEATURES             Location/Qualifiers
     source          1..11763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..341
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1205..1214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1226..1246
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1250..1270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1274..1294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1298..1318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3938..3994
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4010..4066
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4082..4138
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4154..4210
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4226..4282
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4298..4354
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4370..4426
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4442..4498
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4514..4570
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4586..4642
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(4866..4886)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4871..5200
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5051..5186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5210..5584
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    5670..6258
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5723..5743)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6141..6152)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6345..6578
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6650..6771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6790..6925
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6957..6976)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6958..6976)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6986..7003)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6986..7002)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6995..7017)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7147..7602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7234..7253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7444..7465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7631..7735
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7736..7804,7805..8596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7954..7973)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8767..9355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9256..9275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9509..9526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9643..9664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9679..9684,9685..9702,9703..9709)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9717..9733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9722..9744
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9741..9757
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9741..9757
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9776..9796
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9778..9796
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             10052..10232
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10276..10401
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10894..11127
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11312..11356
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11623..11740
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctagcccggg ctcgaccata tgataagctt tgcaaagatg gataaagttt taaacagaga
       61 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      121 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      181 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      241 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      301 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      361 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      421 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      481 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      541 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      601 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      661 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      721 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      781 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      841 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
      901 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
      961 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1021 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1081 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1141 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctcctttc
     1201 tagagccacc atgggtggcg cgcctccaaa aaagaagaga aaggtagatc caaaaaagaa
     1261 gagaaaggta gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtagg
     1321 attaattaat ggaagtggaa gtggaagtgg aggtaccatg tcgcggaccc ggctcccttc
     1381 cccacccgca cccagcccag cgttttcggc cgactcgttc tcagacctgc ttaggcagtt
     1441 cgacccctca ctgtttaaca catcgttgtt cgactccctt cctccgtttg gggcgcacca
     1501 tacggaggcg gccaccgggg agtgggatga ggtgcagtcg ggattgagag ctgcggatgc
     1561 accaccccca accatgcggg tggccgtcac cgctgcccga ccgccgaggg cgaagcccgc
     1621 accaaggcgg agggcagcgc aaccgtccga cgcaagcccc gcagcgcaag tagatttgag
     1681 aactttggga tattcacagc agcagcagga aaagatcaag cccaaagtga ggtcgacagt
     1741 cgcgcagcat cacgaagcgc tggtgggtca tgggtttaca catgcccaca tcgtagcctt
     1801 gtcgcagcac cctgcagccc ttggcacggt cgccgtcaag taccaggaca tgattgcggc
     1861 gttgccggaa gccacacatg aggcgatcgt cggtgtgggg aaacagtgga gcggagcccg
     1921 agcgcttgag gccctgttga cggtcgcggg agagctgaga gggcctcccc ttcagctgga
     1981 cacgggccag ttgctgaaga tcgcgaagcg gggaggagtc acggcggtcg aggcggtgca
     2041 cgcgtggcgc aatgcgctca cgggagcacc cctcaacctg accccagagc aggtcgtggc
     2101 cattgcctcg aatggagggg gcaaacaggc gttggaaacc gtacaacgat tgctgccggt
     2161 gctgtgccaa gcgcacggac tcaccccaga gcaggtcgtg gccattgcct cgaatggagg
     2221 gggcaaacag gcgttggaaa ccgtacaacg attgctgccg gtgctgtgcc aagcgcacgg
     2281 cctcacccca gagcaggtcg tggcaatcgc ctccaacatt ggcgggaaac aggcactcga
     2341 gactgtccag cgcctgcttc ccgtgctgtg ccaagcgcac ggattaaccc cagagcaggt
     2401 cgtggccatt gcctcgaatg gagggggcaa acaggcgttg gaaaccgtac aacgattgct
     2461 gccggtgctg tgccaagcgc acggcttaac cccagagcag gtcgtggcca ttgcctcgaa
     2521 tggagggggc aaacaggcgt tggaaaccgt acaacgattg ctgccggtgc tgtgccaagc
     2581 gcacggactt accccagagc aggtcgtggc gatcgcaagc cacgacggag gaaagcaagc
     2641 cttggaaaca gtacagaggc tgttgcctgt gctgtgccaa gcgcacggcc ttaccccaga
     2701 gcaggtcgtg gcgatcgcaa gccacgacgg aggaaagcaa gccttggaaa cagtacagag
     2761 gctgttgcct gtgctgtgcc aagcgcacgg actaacccca gagcaggtcg tggcgatcgc
     2821 aagccacgac ggaggaaagc aagccttgga aacagtacag aggctgttgc ctgtgctgtg
     2881 ccaagcgcac gggctcaccc cagagcaggt cgtggccatt gcctcgaatg gagggggcaa
     2941 acaggcgttg gaaaccgtac aacgattgct gccggtgctg tgccaagcgc acgggctaac
     3001 cccagagcag gtcgtggcaa tcgcgagcaa taacggcgga aaacaggctt tggaaacggt
     3061 gcagaggctc cttccagtgc tgtgccaagc gcacggccta accccagagc aggtcgtggc
     3121 aatcgcctcc aacattggcg ggaaacaggc actcgagact gtccagcgcc tgcttcccgt
     3181 gctgtgccaa gcgcacgggt taaccccaga gcaggtcgtg gcgatcgcaa gccacgacgg
     3241 aggaaagcaa gccttggaaa cagtacagag gctgttgcct gtgctgtgcc aagcgcacgg
     3301 actcacgcct gagcaggtag tggctattgc atccaatatc gggggcagac ccgcactgga
     3361 gtcaatcgtg gcccagcttt cgaggccgga ccccgcgctg gccgcactca ctaatgatca
     3421 tcttgtagcg ctggcctgcc tcggcggacg acccgccttg gatgcggtga agaaggggct
     3481 cccgcacgcg cctgcattga ttaagcggac caacagaagg attcccgaga ggacatcaca
     3541 tcgagtggca gatcacgcgc aagtggtccg cgtgctcgga ttcttccagt gtcactccca
     3601 ccccgcacaa gcgttcgatg acgccatgac tcaatttggt atgtcgagac acggactgct
     3661 gcagctcttt cgtagagtcg gtgtcacaga actcgaggcc cgctcgggca cactgcctcc
     3721 cgcctcccag cggtgggaca ggattctcca agcgagcggt atgaaacgcg cgaagccttc
     3781 acctacgtca actcagacac ctgaccaggc gagccttcat gcgttcgcag actcgctgga
     3841 gagggatttg gacgcgccct cgcccatgca tgaaggggac caaactcgcg cgtcagctgg
     3901 atccgccatc atacacgcgg ccgcaacaag cggtccggaa gaacttttga gcaagaatta
     3961 tcatcttgag aacgaagtgg ctcgtcttaa gaaaggttct ggcagtggag aagaactgct
     4021 ttcaaagaat taccacctgg aaaatgaggt agctagactg aaaaagggga gcggaagtgg
     4081 ggaggagttg ctgagcaaaa attatcattt ggagaacgaa gtagcacgac taaagaaagg
     4141 gtccggatcg ggtgaggagt tactctcgaa aaattatcat ctcgaaaacg aagtggctcg
     4201 gctaaaaaag ggcagtggtt ctggagaaga gctattatct aaaaactacc acctcgaaaa
     4261 tgaggtggca cgcttaaaaa agggaagtgg cagtggtgaa gagctactat ccaagaatta
     4321 tcatcttgag aacgaggtag cgcgtttgaa gaagggttcc ggctcaggag aggaactgct
     4381 ctcgaagaac tatcatcttg aaaatgaggt cgctcgatta aaaaagggat cgggcagtgg
     4441 tgaggaacta ctttcaaaga attaccacct cgaaaacgaa gtagctcgat taaagaaagg
     4501 ttcagggtcg ggtgaagaat tactgagtaa aaattatcat ctggaaaatg aggtagcgag
     4561 actaaaaaag gggagtggtt ctggcgagga attgctatcg aaaaattatc atcttgagaa
     4621 cgaagttgct aggctcaaaa agggctcagg ctcaggctga gtttaaacat cgcctgcagg
     4681 ggaagtggaa gtgatatcgg aagtggaagc actagtggaa gtttcgaagt cgacttgttt
     4741 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4801 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatgtcgac
     4861 cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4921 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4981 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5041 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5101 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     5161 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca tggccaagtt
     5221 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     5281 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     5341 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     5401 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     5461 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     5521 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     5581 ctgaccgatc gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     5641 accccacctg taggtttggc aagctcgaca atcaacctct ggattacaaa atttgtgaaa
     5701 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5761 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5821 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5881 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5941 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6001 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6061 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6121 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6181 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6241 tttgggccgc ctccccgcct ggaattcgag ctcggaacct ttaagaccaa tgacttacaa
     6301 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6361 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6421 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6481 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6541 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6601 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     6661 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6721 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6781 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     6901 tagtgaggag gcttttttgg aggcctaggc ttttgcgtcg agacgtaccc aattcgccct
     6961 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7021 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7081 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7141 ggcgcgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7201 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7261 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7321 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7381 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7441 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7501 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7561 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccaggtg gcacttttcg
     7621 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7681 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7741 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7801 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7861 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7921 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7981 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8041 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8101 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8161 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8221 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8281 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8341 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8401 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8461 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8521 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8581 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8641 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8701 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8761 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8821 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8881 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8941 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9001 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9061 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9421 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9481 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9541 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9601 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9661 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9721 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     9781 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     9841 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     9901 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     9961 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
    10021 gtatttaagt gcctagctcg atacaataaa cgggtctctc tggttagacc agatctgagc
    10081 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    10141 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    10201 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cctgaaagcg
    10261 aaagggaaac cagagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    10321 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    10381 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    10441 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    10501 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    10561 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    10621 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    10681 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    10741 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    10801 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    10861 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    10921 agcagcagga agcactatgg gcgcagcctc aatgacgctg acggtacagg ccagacaatt
    10981 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    11041 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    11101 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    11161 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    11221 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    11281 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    11341 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    11401 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    11461 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    11521 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    11581 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg
    11641 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    11701 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct
    11761 cga
//
