LOCUS       sequence-246047-        3575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Stat92E-GFP in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pMT Stat92E-GFP
FEATURES             Location/Qualifiers
     source          1..3575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..36
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             2353..3066
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2353..3063
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(2380..2408)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3003..3023
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(3086..3103)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3105..3239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3129..3148)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3183..3202
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gaagatcaag ttgacccaag acttattgac ggaaaaggag gaggaggccg gcccatgagc
       61 ttgtggaagc gcatctccag ccatgtcgac tgcgagcagc gtatggcggc ttactacgag
      121 gagaagggta tgctcgagct gcgcctctgc ttggcaccct ggatcgaaga caggataatg
      181 tccgaacaaa taacgcccaa cactaccgat cagttagagc gcgtggccct aaagttcaac
      241 gaagatctgc agcaaaagct tctatccacg cgcaccgcca gcgaccaggc cctcaagttc
      301 cgggtggtgg agctttgcgc cctcatccaa cgcatctccg ccgttgagct gtacacgcat
      361 ctgcgcagcg gtctacaaaa agagttgcag ttggtcaccg agaagagcgt ggctgcaacc
      421 gcaggccaat caatgccgct aaatccctac aacatgaaca acacgcccat ggttaccggg
      481 tacatggtgg accccagtga tcttctggcg gtgtccaaca gctgcaatcc tccagttgtt
      541 cagggcatag gacctatcca caatgtgcaa aatacaggca ttgcctcccc agccctcggt
      601 atggtcacac ccaaggtgga gctgtacgag gtgcaacatc agatcatgca gtccctcaat
      661 gagtttggca actgtgccaa tgccttgaag ctgcttgccc aaaactacag ttacatgcta
      721 aactccacat cctcgccgaa cgcagaagct gcctacagaa gtcttatcga tgaaaaggcg
      781 gccatcgtac tcacaatgcg tcgcagcttt atgtactacg aatcgctgca cgagatggtc
      841 atacacgagc tcaagaactg gcgccaccaa caagcgcaag ccggaaacgg agctcccttt
      901 aatgagggct ccctggatga tatccagcgc tgctttgaga tgctcgagag ctttatcgca
      961 cacatgctgg ctgccgttaa ggagctgatg cgcgtacgtc tggtcaccga agagccggag
     1021 ctcacacatc tgcttgaaca ggtgcagaac gcgcagaaga acctggtatg ctctgccttt
     1081 atcgtcgaca aacagccccc gcaagtgatg aagaccaaca cacgcttcgc agcgtctgtg
     1141 cgttggctaa tcggttctca gctgggcatt cacaacaatc cacccacagt cgagtgcatc
     1201 ataatgtcag agatccagtc gcaacggttc gttacgcgca atacacagat ggataatagt
     1261 agcctatccg gtcaatcatc gggcgagatt caaaacgcct ctagcactat ggagtatcag
     1321 caaaacaacc atgttttctc cgccagcttc cgaaacatgc agctgaagaa gatcaagcgg
     1381 gcagaaaaga agggcactga gagcgtgatg gacgagaagt tcgccctgtt cttctacacc
     1441 accaccaccg tgaacgattt ccagatccgt gtgtggaccc tgtccttacc ggtggtggtt
     1501 attgtgcacg gcaaccagga gccccagtct tgggctacca ttacttggga caatgcattt
     1561 gcggagattg ttcgcgatcc cttcatgatt accgaccgcg ttacctgggc ccagctgtca
     1621 gtcgcgttga acatcaaatt cggatcgtgc acagggcgtt ctctgaccat tgataacctt
     1681 gatttcctat acgagaaact ccagcgtgag gagcggtctg agtacattac gtggaaccaa
     1741 ttctgtaagg agcccatgcc cgatcggtcc tttacattct gggagtggtt ctttgccatc
     1801 atgaaactca ccaaagatca catgttgggc atgtggaaag caggctgcat tatgggcttc
     1861 atcaacaaga ccaaggctca gactgatctg ctgcgttcag tctatggtat cggcactttc
     1921 ctgctccgtt tctccgacag cgagctcggt ggagtcacta tcgcctacgt aaacgaaaat
     1981 ggactggtca ccatgctagc gccatggact gcacgggatt tccaggtgct gaacctggcc
     2041 gatcgcattc gagatctgga cgtgctttgc tggctgcacc ctagcgaccg caatgcgagt
     2101 cccgtgaaga gggacgtcgc cttcggtgag ttctactcaa agcgtcaaga tcctgtgacc
     2161 ggttatgtga agagcacatt gcatgttcac gtttgtagaa acggagagaa tggatctact
     2221 agtggaacac cgcatcatgc tcaggaaagc atgcaactgg gaaatggtga ctttggaatg
     2281 gcggacttcg atacgattac aaactttgag aactttgggc gcgccggcgg ccgctcgagt
     2341 ctagagggcc ccagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     2401 ttagatggtg atgttaatgg gcacaaattt tctgtccgtg gagagggtga aggtgatgct
     2461 acaaacggaa aactcaccct taaatttatt tgcactactg gaaaactacc tgttccgtgg
     2521 ccaacacttg tcactactct gacctatggt gttcaatgct tttcccgtta tccggatcac
     2581 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact
     2641 atatctttca aagatgacgg gacctacaag acgcgtgctg aagtcaagtt tgaaggtgat
     2701 acccttgtta atcgtatcga gttaaagggt attgatttta aagaagatgg aaacattctt
     2761 ggacacaaac tcgagtacaa ctttaactca cacaatgtat acatcacggc agacaaacaa
     2821 aagaatggaa tcaaagctaa cttcaaaatt cgccacaacg ttgaagatgg ttccgttcaa
     2881 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     2941 aaccattacc tgtcgacaca atctgtcctt tcgaaagatc ccaacgaaaa gcgtgaccac
     3001 atggtccttc ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac
     3061 aaataggttt aaacccgctg atcagcctcg actgtgcctt ctaagatcca gacatgataa
     3121 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3181 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3241 actctagagg atccggtgcg tggtggtttc atgcttctgg gaacggcaaa tgggtttagg
     3301 attgggaacc cctcatcatc tgttggaata tactattcaa cctacaaaag taacgttaaa
     3361 caacactact ttatatttga tatgaatggc cacacctttt atgccataaa acatattgta
     3421 agagaatacc actcttttta ttccttcttt ccttcttgta cgttttttgc tgtaagtagg
     3481 tcgtggtgct ggtgttgcag ttgaaataac ttaaaatata aatcataaaa ctcaaacata
     3541 aacttgacta tttatttatt tattaagaaa ggaaa
//
