LOCUS       sequence-246057-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of Lifeact-TagBFP in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pMT Lifeact-TagBFP
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..72
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
ORIGIN
        1 atcggggtac ctactagtca aatgggtgtc gcagatttga tcaagaaatt cgaaagcatc
       61 tcaaaggaag aaggttctgg ttctatgagc gagctgatta aggagaacat gcacatgaag
      121 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
      181 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
      241 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
      301 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
      361 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
      421 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
      481 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
      541 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
      601 aacatcaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
      661 tactatgtgg actacagact ggaaaga
//
