LOCUS       sequence-24606-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Trivedi RN, Wang XH, Jelezcova E, Goellner EM, Tang J, Sobol RW
  TITLE     Human methyl purine DNA glycosylase and DNA polymerase {beta} 
            expression collectively predict sensitivity to temozolomide.
  JOURNAL   Molecular Pharmacology 2008;74(2):505-16.
  PUBMED    18477668
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Molecular 
            Pharmacology"; date: "2008"; volume: "74(2)"; pages: "505-16"
COMMENT     Sequence Label: pFIV-H1-Puro-hPolß
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(71..87)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(71..87)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(84..106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    95..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(119..125,126..143,144..149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    164..185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(302..319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(553..572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cncnnnnntc ngagngtgag gaggcttttt tggaggccta gacttttgca gagaccaaat
       61 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      721 gctgtgtgca ccnnncccct tttaagccng nccgnntgcc tttatcnggt anctatcgtc
      781 ttgagtc
//
