LOCUS       sequence-246060-        1885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of a synthetic FRB-Draper receptor in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pMT FRB-Draper INT-mCherry
FEATURES             Location/Qualifiers
     source          1..1885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..51
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             133..411
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             1183..1884
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELY"
     primer_bind     complement(1325..1343)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1593..1612
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 catcaccatc atcacgaaga tcaagttgac ccaagactta ttgacggaaa aggaggagga
       61 ggccggccca tgatgtctcg ctccgtggcc ttagctgtgc tcgcgctact ctctctttct
      121 ggcctggagg caatcctctg gcatgagatg tggcatgaag gcctggaaga ggcatctcgt
      181 ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct
      241 atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga
      301 gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac
      361 ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa gggtagcggt
      421 agcggtagcg gtggaggtgg aagcagcgcg agtgtagctc tgaccttagt cctgatgacc
      481 ctgtttgcgt gcataatctt cgcagtgttc atttactacc gccgacgtgt gtccaatcta
      541 aaaaccgaaa tcgcccatgt acactacacc catgatacga atcccccgag ctggccgccc
      601 aaccacaact tcgacaatcc cgtgtacggc atgcaggcgg aaacgcgtct cctgcccaac
      661 aatatgcgtt ccaagatgaa caactttgac cagaggagca cgatgagcac ggactacggc
      721 gatgactgca atgccagtgg cagggtgggt agctattcga tcaactacaa ccacgacctg
      781 ctaacgaaga acttgaacgc ggaccggacc aatccgattg tgtacaacga atccctcaaa
      841 gaggagcatg tctacgacga gatcaagcac aaggagggct acaaggatcc cgatgaatac
      901 gatcacctgg actactcacg acccagcacc tcgcagaagc cgcactacca ccgcatgaac
      961 gacgccatgc tcaacatcaa ccaggacgag gagaagccca gcaacgtgaa gaacatgact
     1021 gtgctgctga acaaaccgct gccgcccacg gaaccggagc cacaacacga gtgcttcgac
     1081 aacacgaaca ccaacctgga caacgtgtcg acggcgtcgc ctagttccag tccgaaattt
     1141 ctaaagcggc gcgccggcgg ccgctcgagt ctagagggcc ccgtgagcaa gggcgaggag
     1201 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     1261 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1321 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     1381 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     1441 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1501 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     1561 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     1621 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     1681 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     1741 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     1801 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     1861 accggcggca tggacgagct gtaca
//
