LOCUS       sequence-246063-       10224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry plasmid for guide cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity 
            AJ, Zhang F, Blainey PC
  TITLE     Optical Pooled Screens in Human Cells.
  JOURNAL   Cell. 2019 Oct 17;179(3):787-799.e17. doi: 
            10.1016/j.cell.2019.09.016.
  PUBMED    31626775
REFERENCE   2  (bases 1 to 10224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.09"; journalName: "Cell"; date: "2019-10-17- 
            17"; volume: "179"; issue: "3"; pages: "787-799.e17"
COMMENT     Sequence Label: CROPseq-puro-v2
FEATURES             Location/Qualifiers
     source          1..10224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1489..1606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1745..2923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1976..2914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2871..2891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2936..3535
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2936..2955)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3432..3452
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3551..4139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3604..3624)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4022..4033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4295..4543
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4295..4315
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4466..4485
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             6563..6743
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6821..6942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6858..6877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6912..6931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6982..7117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7137..7156)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7138..7156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7166..7183)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7166..7182)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7175..7197)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7324..7779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7411..7430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7621..7642
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7805..7909
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7910..7978,7979..8770)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8128..8147)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8941..9529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9430..9449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9683..9700
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9817..9838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9853..9858,9859..9876,9877..9883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9891..9907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9896..9918
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9915..9931
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9915..9931
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9950..9970
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9952..9970
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9998..10224
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     1501 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     1561 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     1621 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa
     1681 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     1741 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1801 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     1861 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1921 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1981 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     2041 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     2101 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     2161 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     2221 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     2281 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     2341 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     2401 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     2461 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2521 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2581 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2641 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2701 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2761 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2821 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2881 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgtacgg ccaccatgac
     2941 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     3001 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     3061 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     3121 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     3181 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     3241 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     3301 gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     3361 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     3421 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     3481 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg
     3541 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3601 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3661 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3721 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3781 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3841 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3901 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     3961 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4021 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4081 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     4141 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4201 aaggggggac tggaagggct aattcactcc caacgaagac aagatttcac cattatcgtt
     4261 tcagacccac ctcccaaccc cgaggggacc cagagagggc ctatttccca tgattccttc
     4321 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
     4381 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     4441 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     4501 cgatttcttg gctttatata tcttgtggaa aggacgaaac accggagacg gttgtaaatg
     4561 agcacacaaa atacacatgc taaaatatta tattctatga cctttataaa atcaaccaaa
     4621 atcttctttt taataacttt agtatcaata attagaattt ttatgttcct ttttgcaaac
     4681 ttttaataaa aatgagcaaa ataaaaaaac gctagtttta gtaactcgcg ttgttttctt
     4741 cacctttaat aatagctact ccaccacttg ttcctaagcg gtcagctcct gcttcaatca
     4801 ttttttgagc atcttcaaat gttctaactc caccagctgc tttaactaaa gcattgtctt
     4861 taacaactga cttcattagt ttaacatctt caaatgttgc acctgatttt gaaaatcctg
     4921 ttgatgtttt aacaaattct aatccagctt caacagctat ttcacaagct ttcatgattt
     4981 cttcttttgt taataaacaa ttttccataa tacatttaac aacatgtgat ccagctgctt
     5041 tttttacagc tttcatgtct tctaaaacta attcataatt tttgtctttt aatgcaccaa
     5101 tatttaatac catatcaatt tctgttgcac catctttaat tgcttcagaa acttcgaatg
     5161 cttttgtagc tgttgtgcat gcacctagag gaaaacctac aacatttgtt attcctacat
     5221 ttgtgccttt taataattct ttacaatagc ttgttcaata tgaattaaca caaactgttg
     5281 caaaatcaaa ttcaattgct tcatcacata attgtttaat ttcagctttc gtagcatctt
     5341 gttttaataa tgtgtgatct atatatttgt ttagtttcat tttttctcct atatattcat
     5401 ttttaatttt aattctttaa taatttcgtc tactttaact ttagcgtttt gaacagattc
     5461 accaacacct ataaaataaa tttttagttt aggttcagtt ccacttgggc gaacagcaaa
     5521 tcatgactta tcttctaaat aaaattttag taagtcttgt cctggcatat tatacattcc
     5581 atcgatgtag tcttcaacat taacaacttt aagtccagca atttgagtta agggtgttgc
     5641 tctcaatgat ttcattaatg gttcaatttt taatttcttt tcttctggtt taaaattcaa
     5701 gtttaaagtg aaagtgtaat atgcacccat ttctttaaat aaatcttcta aatagtctac
     5761 taatgtttta ttttgttttt tataaaatca agcagcctct gctattaata tagaagcttg
     5821 tattccatct ttatctctag ctgagtcatc aattacatat ccataacttt cttcataagc
     5881 aaaaacaaaa tttaatccgt tatcttcttc tttagcaatt tctctaccca ttcatttaaa
     5941 tccagttaaa gtttttacaa tattaactcc atatttttca tgagcgattc tatcacccaa
     6001 atcacttgtt acaaaacttg aatatagagc cggatttttt ggaatgctat ttaagcgttt
     6061 tagatttgat aattttcaat caattaaaat tggtcctgtt tgatttccat ctaatcttac
     6121 aaaatgacca tcatgtttta ttgccattcc aaatctgtca gcatctgggt cattcataat
     6181 aataatatct gcatcatgtt taataccata ttcaagcggt atttttcatg caggatcaaa
     6241 ttctggattt ggatttacaa catttttaaa tgtttcatct tcaaatgcat gctcttcaac
     6301 ctcaataacg ttatatcctg attcacgtaa tatttttggg gtaaatttag ttcctgttcc
     6361 attaactgcg ctaaaaataa tttttaaatc ttttttagct tcttgctctt ttttgtacgt
     6421 ctctgtttca gagctatgct ggaaacagca tagcaagttg aaataaggct agtccgttat
     6481 caacttgaaa aagtggcacc gagtcggtgc ttttttaagc ttggcgtaac tagatcttga
     6541 gacactgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6601 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6661 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6721 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     6781 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     6841 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6901 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     6961 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7021 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7081 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     7141 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7201 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7261 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7321 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7381 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7441 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7501 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7561 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7621 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7681 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7741 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7801 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7861 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7921 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7981 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8041 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8101 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8161 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8221 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8281 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8341 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8401 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8461 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8521 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8581 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8641 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8701 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8761 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8821 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8881 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8941 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9001 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9061 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9121 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9181 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9241 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9301 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9361 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9421 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9481 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9541 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9601 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9661 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9721 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9781 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9841 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9901 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     9961 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
    10021 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
    10081 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
    10141 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
    10201 aagtgcctag ctcgatacat aaac
//
