LOCUS       sequence-246065-       11772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9-15xFlag-TdT in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, 
            Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC
  TITLE     Lineage tracing and analog recording in mammalian cells by 
            single-site DNA writing.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 
            10.1038/s41589-021-00769-8. Epub 2021 Mar 22.
  PUBMED    33753928
REFERENCE   2  (bases 1 to 11772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00769-8"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "739-747"
COMMENT     Sequence Label: pTBL469
FEATURES             Location/Qualifiers
     source          1..11772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      911..920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             935..5038
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             935..5038
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             935..5038
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5051..5071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5078..5209
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     CDS             5261..5392
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     CDS             5447..5578
                     /codon_start=1
                     /product="five tandem FLAG(R) epitope tags"
                     /label=5xFLAG
                     /translation="DYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDK"
     primer_bind     complement(7207..7224)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7213..7437
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7483..7911
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7570..7589)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7780..7801
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7920..7940)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7925..8254
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8105..8240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8167..8186
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9445..9566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9482..9501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9536..9555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9615..9631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9615..9631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9628..9650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9639..9655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9663..9669,9670..9687,9688..9693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9708..9729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9846..9863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10017..10605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10097..10116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10776..11567,11568..11636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11399..11418
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11637..11741)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cctgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctaccggtc gccaccatgg gccctagtgg cccaatggac aagaagtact ccattgggct
      961 cgatatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag
     1021 caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg
     1081 cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg
     1141 gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga
     1201 gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga
     1261 ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca
     1321 tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc
     1381 tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct
     1441 catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt
     1501 tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa
     1561 agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct
     1621 ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac
     1681 ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga
     1741 cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct
     1801 ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa
     1861 cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca
     1921 ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga
     1981 aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca
     2041 ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct
     2101 gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag
     2161 catcccccac cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt
     2221 ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc
     2281 ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc
     2341 agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca
     2401 gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc
     2461 taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata
     2521 cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt
     2581 ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt
     2641 caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc
     2701 atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa
     2761 tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag
     2821 ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa
     2881 acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg
     2941 gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc
     3001 caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca
     3061 gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg
     3121 tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa
     3181 agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac
     3241 tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa
     3301 agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga
     3361 gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga
     3421 catcaatcgg ctctccgact acgacgtgga tcatatcgtg ccccagtctt ttctcaaaga
     3481 tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa
     3541 cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc
     3601 caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc
     3661 tgagttggat aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa
     3721 gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact
     3781 gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga
     3841 ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct
     3901 gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt
     3961 ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat
     4021 aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga
     4081 gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac
     4141 aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat
     4201 gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag
     4261 tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa
     4321 gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt
     4381 ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat
     4441 ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga
     4501 ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg
     4561 ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc
     4621 ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc
     4681 cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat
     4741 catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa
     4801 ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat
     4861 tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac
     4921 caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca
     4981 tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacag
     5041 cagggctgac cccaagaaga agaggaaggt gggtaccgac tacaaggacg acgatgacaa
     5101 ggattacaaa gatgacgacg ataagcttat ggactacaag gacgacgatg acaaggatta
     5161 caaagatgac gacgataagc ttatggacta caaggacgac gatgacaagc atatgggcgc
     5221 gccacttcta aataagcgaa tttcttatga tttaggtacc gactacaagg acgacgatga
     5281 caaggattac aaagatgacg acgataagct tatggactac aaggacgacg atgacaagga
     5341 ttacaaagat gacgacgata agcttatgga ctacaaggac gacgatgaca agcatatggg
     5401 cgcgccactt ctaaataagc gaatttctta tgatttaggc cggccagact acaaggacga
     5461 cgatgacaag gattacaaag atgacgacga taagcttatg gactacaagg acgacgatga
     5521 caaggattac aaagatgacg acgataagct tatggactac aaggacgacg atgacaagca
     5581 tatgggcgcg ccacttctaa ataagcgaat ttcttatgat ttaggccggc caatggatcc
     5641 accacgagcg tcccacttga gccctcggaa gaagagaccc cggcagacgg gtgccttgat
     5701 ggcctcctct cctcaagaca tcaaatttca agatttggtc gtcttcattt tggagaagaa
     5761 aatgggaacc acccgcagag cgttcctcat ggagctggcc cgcaggaaag ggttcagggt
     5821 tgaaaatgag ctcagtgatt ctgtcaccca cattgtagca gagaacaact cgggttcgga
     5881 tgttctggag tggcttcaag cacagaaagt acaagtcagc tcacaaccag agctcctcga
     5941 tgtctcctgg ctgatcgaat gcataggagc agggaaaccg gtggaaatga caggaaaaca
     6001 ccagcttgtt gtgagaagag actattcaga tagcaccaac ccaggccccc cgaagactcc
     6061 accaattgct gtacaaaaga tctcccagta tgcgtgtcag agaagaacca ctttaaacaa
     6121 ctgtaaccag atattcacgg atgcctttga tatactggct gaaaactgtg agtttagaga
     6181 aaatgaagac tcctgtgtga catttatgag agcagcttct gtattgaaat ctctgccatt
     6241 cacaatcatc agtatgaagg acacagaagg aattccctgc ctggggtcca aggtgaaggg
     6301 tatcatagag gagattattg aagatggaga aagttctgaa gttaaagctg tgttaaatga
     6361 tgaacgatat caatccttca aactctttac ttctgtattt ggagtggggc tgaagacttc
     6421 tgagaagtgg ttcaggatgg gtttcagaac tctgagtaaa gtaaggtcgg acaaaagcct
     6481 gaaatttaca cgaatgcaga aagcaggatt tctgtattat gaagaccttg tcagctgtgt
     6541 gaccagggca gaagcagagg ccgtcagtgt gctggttaaa gaggctgtct gggcatttct
     6601 tccggatgct ttcgtcacca tgacaggagg gttccggagg ggtaagaaga tggggcatga
     6661 tgtagatttt ttaattacca gcccaggatc aacagaggat gaagagcaac ttttacagaa
     6721 agtgatgaac ttatgggaaa agaagggatt acttttatat tatgaccttg tggagtcaac
     6781 atttgaaaag ctcaggttgc ctagcaggaa ggttgatgct ttggatcatt ttcaaaagtg
     6841 ctttctgatt ttcaaattgc ctcgtcaaag agtggacagt gaccagtcca gctggcagga
     6901 aggaaagacc tggaaggcca tccgtgtgga tttagttctg tgcccctacg agcgtcgtgc
     6961 ctttgccctg ttgggatgga ctggctcccg gcagtttgag agagacctcc ggcgctatgc
     7021 cacacatgag cggaagatga ttctggataa ccatgcttta tatgacaaga ccaagaggat
     7081 attcctcaaa gcagaaagtg aagaagaaat ttttgcgcat ctgggattgg attatattga
     7141 accgtgggaa agaaatgcct aggcggccgc tcgagtctag agggcccgtt taaacccgct
     7201 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     7261 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     7321 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     7381 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     7441 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     7501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7681 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7801 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7861 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     7921 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7981 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8041 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8101 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8161 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     8221 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     8281 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgcagctct cggagggcga
     8341 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     8401 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
     8461 cccgattccg gaagtgcttg acattgggga atttagcgag agcctgacct attgcatctc
     8521 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     8581 gcaaccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
     8641 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     8701 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     8761 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     8821 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     8881 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     8941 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     9001 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
     9061 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     9121 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     9181 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     9241 ccccagcact cgtccgaggg caaaggaata ggcgggactc tggggttcga aatgaccgac
     9301 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9361 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9421 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     9481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9541 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     9601 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9721 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10021 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10201 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10441 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10741 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10801 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10861 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10921 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10981 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11041 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11101 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11161 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11221 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11281 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11341 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11401 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11461 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11521 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11581 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11641 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11701 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11761 ccaccttacg tc
//
