LOCUS       sequence-246067-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of mCherry-CaaX in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pMT mCherry-CaaX
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..711
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(149..167)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     417..436
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcaatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
       61 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      121 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      181 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      241 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      301 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      361 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      421 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      481 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      541 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      601 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      661 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gctcgagaaa
      721 atgtccaagg atggtaagaa aaagaagaag aagtcaaaaa ccaagtgtgt t
//
