LOCUS       sequence-246068-         675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of His10-Draper intracellular domain for purification 
            from E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pET His10-Draper INT
FEATURES             Location/Qualifiers
     source          1..675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..42
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             52..69
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcat catcaccatc acagcagcgg cctggtgccg
       61 cgcggcagcc atatgcgccg acgtgtgtcc aatctaaaaa ccgaaatcgc ccatgtacac
      121 tacacccatg atacgaatcc cccgagctgg ccgcccaacc acaacttcga caatcccgtg
      181 tacggcatgc aggcggaaac gcgtctcctg cccaacaata tgcgttccaa gatgaacaac
      241 tttgaccaga ggagcacgat gagcacggac tacggcgatg actgcaatgc cagtggcagg
      301 gtgggtagct attcgatcaa ctacaaccac gacctgctaa cgaagaactt gaacgcggac
      361 cggaccaatc cgattgtgta caacgaatcc ctcaaagagg agcatgtcta cgacgagatc
      421 aagcacaagg agggctacaa ggatcccgat gaatacgatc acctggacta ctcacgaccc
      481 agcacctcgc agaagccgca ctaccaccgc atgaacgacg ccatgctcaa catcaaccag
      541 gacgaggaga agcccagcaa cgtgaagaac atgactgtgc tgctgaacaa accgctgccg
      601 cccacggaac cggagccaca acacgagtgc ttcgacaaca cgaacaccaa cctggacaac
      661 gtgtcgacgg cgtcg
//
