LOCUS       sequence-246071-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of His10-Draper delta ITAM mutant for purification from 
            E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pET His10-Draper delta ITAM
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..42
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             52..69
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             73..105
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcat catcaccatc acagcagcgg cctggtgccg
       61 cgcggcagcc atatggctag catgactggt ggacagcaaa tgggtcgcgg atcccgccga
      121 cgtgtgtcca atctaaaaac cgaaatcgcc catgtacact acacccatga tacgaatccc
      181 ccgagctggc cgcccaacca caacttcgac aatcccgtgt acggcatgca ggcggaaacg
      241 cgtctcctgc ccaacaatat gcgttccaag atgaacaact ttgaccagag gagcacgatg
      301 agcacggact acggcgatga ctgcaatgcc agtggcaggg tgggtagcta ttcgatcaac
      361 tacaaccacg acctgctaac gaagaacttg aacgcggacc ggaccaatcc gattgtgtac
      421 aacgaatccc tcaaagagga gcatgtcttc gacgagatca agcacaagga gggctacaag
      481 gatcccgatg aattcgatca cctggactac tcacgaccca gcacctcgca gaagccgcac
      541 taccaccgca tgaacgacgc catgctcaac atcaaccagg acgaggagaa gcccagcaac
      601 gtgaagaaca tgactgtgct gctgaacaaa ccgctgccgc ccacggaacc ggagccacaa
      661 cacgagtgct tcgacaacac gaacaccaac ctggacaacg tgtcgacggc gtcgcctagt
      721 tccagtccga aatttctaaa gtaagaattc gagctccgtc gacaagcttg c
//
