LOCUS       sequence-246072-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Shark tandem SH2 domains SNAP tagged for use as FRET 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Williamson AP, Vale RD
  TITLE     Spatial control of Draper receptor signaling initiates apoptotic 
            cell engulfment.
  JOURNAL   J Cell Biol. 2018 Nov 5;217(11):3977-3992. doi: 
            10.1083/jcb.201711175. Epub 2018 Aug 23.
  PUBMED    30139739
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201711175"; journalName: "J Cell Biol"; date: 
            "2018-11-5- 5"; volume: "217"; issue: "11"; pages: "3977-3992"
COMMENT     Sequence Label: pGEX Shark-tSH2
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..585
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for bacterial expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 atcggatctg gagttctgtt ccaggggccc ctgggatcca tggacaaaga ttgcgaaatg
       61 aaacgtacca ccctggatag cccgctgggc aaactggaac tgagcggctg cgaacagggc
      121 ctgcatgaaa ttaaactgct gggtaaaggc accagcgcgg ccgatgcggt tgaagttccg
      181 gccccggccg ccgtgctggg tggtccggaa ccgctgatgc aggcgaccgc gtggctgaac
      241 gcgtattttc atcagccgga agcgattgaa gaatttccgg ttccggcgct gcatcatccg
      301 gtgtttcagc aggagagctt tacccgtcag gtgctgtgga aactgctgaa agtggttaaa
      361 tttggcgaag tgattagcta tcagcagctg gcggccctgg cgggtaatcc ggcggccacc
      421 gccgccgtta aaaccgcgct gagcggtaac ccggtgccga ttctgattcc gtgccatcgt
      481 gtggttagct ctagcggtgc ggttggcggt tatgaaggtg gtctggcggt gaaagagtgg
      541 ctgctggccc atgaaggtca tcgtctgggt aaaccgggtc tgggaggtag cggtagcggt
      601 agcggtggag gtggaagcag cactagaatg agtcgcgata gtgatcccat gaaatggtac
      661 cacggcaacc tgtcccgcga ggcagccgac gaactgctaa agcagggtta cgaggacggg
      721 acatttctgg tgcgcgagag cagcacagcg gccggggact ttgtgctgag cctgctctgc
      781 cagggtgagg tgtgtcacta ccaggtgcgg cgacatggcg gcgaggacgc cttcttctcc
      841 atcgacgaca aggtacagac gaagatcctg cacggc
//
