LOCUS       sequence-246077-        9432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite (attR3/attR4) Gateway vector for expression in oomycetes. 
            Confers resistance to geneticin (G418). Contains additional cassette
            for constitutive expression of tdTomato..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Evangelisti E, Shenhav L, Yunusov T, Le Naour-Vernet M, Rink P, 
            Schornack S
  TITLE     Hydrodynamic Shape Changes Underpin Nuclear Rerouting in Branched 
            Hyphae of an Oomycete Pathogen.
  JOURNAL   MBio. 2019 Oct 1;10(5). pii: mBio.01516-19. doi: 
            10.1128/mBio.01516-19.
  PUBMED    31575765
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-10-1"; volume: "10(5). pii: mBio.01516-19. doi"; pages: 
            " 10.1128/mBio.01516-19"
COMMENT     Sequence Label: pTORKm43GWR
FEATURES             Location/Qualifiers
     source          1..9432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(322..341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(323..341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(348..365)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(348..364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(357..379)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(505..960)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(642..663)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     854..873
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        987..1091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1092..1160,1161..1952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1310..1329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2123..2711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2612..2631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2865..2882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2999..3020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3035..3040,3041..3058,3059..3065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3073..3089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3078..3100
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3097..3113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3097..3113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3134..3152
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3745..3761
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3746..3762
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5917..6040
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(6081..6386)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVFTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(6183..6202)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(6706..7386)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     7301..7320
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(7440..7446,7447..7464,7465..7470))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(7495..7618)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8232..9026
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8286..8305)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8896..8915
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tacgccgcct tagtaacaaa catgccaaaa gaaggaaagc tttcaccagc tttcaagttt
       61 aatcaatctt tgagagtcac aattgcaaga ataaatgaat ttttacaaca cagttcgatg
      121 cactgtgttt cgtcttgatg aaaattggac ggcaaatcga gtatcttaaa caaatctcgg
      181 cataggctgt ccattttgtg gtcgcctctt tgttggtagt ttgcagtagt ttcattagta
      241 gaagatctgc atcctcaatc tgctaaacgt cttgagcagc tatgagacga tccggggaat
      301 tggggcccga gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
      361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      481 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
      541 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
      601 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
      661 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
      721 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
      781 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
      841 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
      901 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
      961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2941 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     3001 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     3061 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     3121 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtacct ctgatggaca
     3181 aagggtcgcc tctacgtacc cgcgacaatc tcggcttttc caatgtcgac agctggcata
     3241 tcgcacgcga ggttaggcgt cttcagccgg cgtaaccaca aacctcgagt caagtaccct
     3301 ttttcagacc ttaatttccg tgtccattcc ctcgcgttta cacgaaggtt tctgagaaat
     3361 accacatgtc acagagagag agaagattgg agaaccttct caacctgacc cgtgcagagc
     3421 gcttttgcgt cctaccatcc gttaattata gcaccaccac cacatgtctc gccggaactc
     3481 tcttatccgc tctttttgat cgtctttttt gcgccccatt aaagacatct cttgcacagg
     3541 gatcgtttct gattggccat ttcacttcct cttttacatc cgaaaaccac ctgtttgtgg
     3601 ggttgtgaag ccacaaccgg acgctcaaaa agcctcgccc gactcgccca cgatcggaag
     3661 gctcattctc cttttcactc tcacgtgccc aagtcccaac cgactctttt tcgaccttca
     3721 ctctcaccga caacgggccc cccctcgagg tcgacggtat cgataccatg gtgagcaagg
     3781 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
     3841 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3901 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     3961 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
     4021 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
     4081 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
     4141 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
     4201 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     4261 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     4321 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
     4381 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     4441 tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg
     4501 acaacaacat ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca
     4561 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     4621 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     4681 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt
     4741 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     4801 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg
     4861 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg
     4921 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc
     4981 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca
     5041 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca
     5101 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc
     5161 acctgttcct ggggcatgga cgagctgtac aagtaaccgc ggggtgccta cttcttgtaa
     5221 gccgaaggtc gagctgtgtg cactgagtgc gaagttgcag attttgtgtc tatgcattta
     5281 atacaatttc cttttttcca ttttcgttgt tttttttgtg aaagaacgat tgttttcaaa
     5341 agaaggccgt ttgtagaaca attttttttt atactagcat ttggtattcg agtgtgacga
     5401 atcggtgatg gcgctttaca aatgaaatat aacgctgaac aagcgcagtg acagctttat
     5461 tttataacag agaataaaga ccaaagtaga gtagactgat ggcggcttga aacaaaacta
     5521 cgtgacttct aatgcaaaga aaaccatacc ccacgctact tagaattgtt tctctttggt
     5581 tgatgaccgc tatagtcgta tggagtgatc cctttttgtc aatatttcga ttcttgctaa
     5641 aaccgtgact attttgaggc aactgtgtgt acttaacatg gtagaaattt ctatgtttaa
     5701 gccagctgac aatcattctt tatttatcgt aaaccaacca ggtaccgggc ccattatacc
     5761 tcgtattcga ttaaatgata caactcattg ccacaccgca agacggcgtc accaccatag
     5821 acagcctttc aagcaccccc ttgtctatga gctgccacgt cagctgccgc tcctaatatc
     5881 cacccgcgtc cctattcccg tcatatgcca agctatcaac tttgtataga aaagttgaac
     5941 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     6001 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg
     6061 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
     6121 tcattttcgc ggtgactgag atcagccact tcttccccga taacggagac cggcacactg
     6181 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
     6241 tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
     6301 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
     6361 ttataggtga aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg caaaagagcc
     6421 gttcatttca ataaaccggg cgacctcagc catcccttct tgattttccg ctttccagcg
     6481 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac cggagatact
     6541 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtgatacgc
     6601 tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta tatattctta
     6661 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc
     6721 ctctagatta cgccccgcct gccactcatc gcagtactgt tgtaattcat taagcattct
     6781 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     6841 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     6901 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     6961 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     7021 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     7081 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     7141 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
     7201 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     7261 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     7321 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     7381 ctccatttta gcttccttag ctcctgaaaa tctcgacgga tcctaactca aaatccacac
     7441 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt
     7501 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     7561 aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata catagttgat
     7621 aattcacata tgctcaatgc aattgactgt gtcctcgatt atttctatta tttaatatta
     7681 tttccattaa aaaagaaatc cagttcgtgg caaaataaga caaaggttta aataaaaatt
     7741 tatcgttatt aaactcgtaa cttaccttct tcatggctca acccaatctg tatatcgact
     7801 tatttaattt ggttaacaaa tcggttttcg tcgcaaatag gggatgacaa ctaaaatacc
     7861 aatttgtgtc cttgcgacac gtcaacaggt ggctgccaat atctcaggtt ttgagcacac
     7921 aacgctgtga ttggctatct gtagatcgct tcgagaactt tcggaccgac atatcagaac
     7981 gcagtcatgt ccaatccagc taacgattgg tcgaaaccgc acttcatcgc ttcatttcaa
     8041 ccaattccca tctactaaaa cgagcgtcgg aatagtctcg aacgtaaccc cacgtagtgg
     8101 gtggcagcgc ccctcgtatc tttcaatcgc cactcgagtc tcaagtttgc tcactttgaa
     8161 attttccatc tggactacac atcaaatcta actcaacttt tatcaattga aacacaaggg
     8221 ccccgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     8281 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     8341 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     8401 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     8461 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     8521 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     8581 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     8641 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     8701 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     8761 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     8821 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     8881 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     8941 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     9001 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     9061 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     9121 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     9181 ggagttcttc gcccaccccc cggatccccg gggtcgacta gaatacttag caattggtta
     9241 gtttgaaaat tgtcaaagca gagttttttc gacaataaaa tttattttct aaatatttga
     9301 tttagcgttg acttaatttg ttgattcggt tgtcatcttt tgtgaagatg cccgagagtc
     9361 tagtaaatct caagcgaaac atagtgagaa ggtcactatg gctggtttgc atgagtctgg
     9421 gcctattgtg ct
//
