LOCUS       sequence-24608-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: CMV-Brainbow-1.0 H
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(54..87)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(104..137)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(293..311)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(343..363)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     561..580
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     757..780
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gnngtnnnnn agnngagctg gtttagtgaa ccgtcagatc cgctagcgag ctcataactt
       61 cgtataggat actttatacg aagttataac gttagatctc gagataactt cgtatagcat
      121 acattatacg aagttatacc ggctagtgat ttcgccacca tggtggcctc ctccgaggac
      181 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag
      241 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      301 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag
      361 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc
      421 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      481 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gttccgcggc
      541 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      601 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agnnnnannn nnnnnnaacn
      661 nnnnnnnngg cgggccacta ccctggtgga gttcaagacc atctacatgg ccaagaagcc
      721 ccgtgcagct gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg
      781 aggactacac catcgtggna cagtacgagc gctccgaggg c
//
